Protein catabolism in mosquitoes: ureotely and uricotely in larval and imaginal Aedes aegypti.

Abstract:

:Catabolism of excess dietary protein by Aedes aegypti was investigated during larval development, during and after metamorphosis. Activity profiles were established for xanthine dehydrogenase (XDH, uricotelic pathway) and arginase (ureotelic pathway). Both enzymes are active at all times during the life-cycle. During the aquatic larval and pupal instars, XDH and arginase activities increase with body size. Maximal activities of these two enzyme systems coincide with the time of metamorphic restructuring.Both enzymes are found in the fatbody tissue: XDH activity is found in 80% of the tissue, while arginase activity is distributed equally between abdominal fatbody and the thorax. This might indicate a role for arginase other than catabolic, such as energy metabolism.Arginase activity is high in the aquatic instars and low in sugar-fed females but increases after blood-feeding. XDH activity, also high in larvae and pupae, increases markedly after a blood meal.Larval excretion is characterized by the ureotelic pathway. The pupae as closed systems excrete neither uric acid nor urea; urate accumulates during larval and pupal periods, is conserved throughout metamorphosis, and is finally voided with the meconium by the teneral imago. This presents a form of transient storage-excretion.

journal_name

J Insect Physiol

authors

von Dungern P,Briegel H

doi

10.1016/s0022-1910(00)00096-2

keywords:

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

131-141

issue

2

eissn

0022-1910

issn

1879-1611

pii

S0022191000000962

journal_volume

47

pub_type

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