Abstract:
:In Dictyostelium discoideum inactivation of developmentally regulated genes via homologous recombination has become an important tool in studying systematically the entire developmental program of this model organism. The Dictyostelium genome is very A/T-rich,which presents obstacles to the preparation of knockout constructs. The coding regions offer few suitable restriction sites and the low complexity intergenic regions do not guarantee specificity of recombination. We present here the preparation of plasmids pBsR479, pBsR503, and pBsR519, in which a blasticidin resistance-cassette is positioned in the center of various symmetrical polylinkers. This design simplifies the cloning process and gives more flexibility in positioning the selectable marker within the coding regions.
journal_name
Folia Biol (Praha)journal_title
Folia biologicaauthors
Půta F,Zeng Ckeywords:
subject
Has Abstractpub_date
1998-01-01 00:00:00pages
185-8issue
5eissn
0015-5500journal_volume
44pub_type
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