Abstract：
:BACKGROUND Photobiomodulation (PBM) has been explored as a promising therapeutic strategy to regulate bone cell growth; however, the effects of PBM on osteoblast cell lines remains poorly understood. In addition, as a light source of PBM, the light uniformity of light-emitting diode (LED) devices has not been given enough attention. MATERIAL AND METHODS Here, we sought to investigate the effects of PBM on MC3T3-E1 cells via 630 nm and 810 nm light from a newly designed LED with high uniformity of light. Cell proliferation, flow cytometric analysis, alkaline phosphatase (ALP) staining, ALP activity, Alizarin Red S staining, and quantitative real-time polymerase chain reaction (qRT-PCR) were carried out to assess treatment response. MC3T3-E1 cells were irradiated with LED devices (630±5 nm and 810±10 nm, continuous wave) for 200 seconds at a power density of 5 mW/cm² once daily. RESULTS Increases in cell proliferation and decreases in cell apoptosis were evident following irradiation. ALP staining intensity and activity were also significantly increased following irradiation. Level of mineralization was obviously enhanced in irradiated groups compared with non-irradiated controls. qRT-PCR also showed significant increases in mRNA expression of osteocalcin (OCN) and osteoprotegerin (OPG) in the irradiated groups. CONCLUSIONS Our results showed that LED PBM could promote the proliferation, ALP staining intensity and activity, level of mineralization, gene expression of OCN and OPG of MC3T3-E1 cells, with no significant difference between the 630 nm- and 810 nm-irradiated groups.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Chang B,Qiu H,Zhao H,Yang X,Wang Y,Ji T,Zhang Y,Quan Q,Li Y,Zeng J,Meng H,Gu Ydoi
10.12659/MSM.920396subject
Has Abstractpub_date
2019-11-19 00:00:00pages
8744-8752eissn
1234-1010issn
1643-3750pii
920396journal_volume
25pub_type
杂志文章abstract：BACKGROUND:To evaluate the per-operative process of shoulderjoint replacement, time-action analysis can be used. MATERIAL/METHODS:Forty procedures performed by 7 surgeons with different experience using 5 different prostheses and 3 different surgical approaches were analyzed. RESULTS:The surgical procedures showed a ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:BACKGROUND This study was conducted to analyze the activity of masticatory muscles depending on the presence of temporomandibular joint disorders (TMD) when foods of various hardness are masticated. MATERIAL AND METHODS We enrolled 20 subjects (13 men and 7 women) who met our selection criteria, and they were divided ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.921337
更新日期：2020-03-10 00:00:00
abstract：:Erythropoietin (EPO) is traditionally described as a hematopoietic cytokine or growth hormone regulating proliferation, differentiation, and survival of erythroid progenitors. The use of EPO in patients with chronic kidney disease (CKD) was a milestone achievement in the treatment of anemia. However, EPO involves some...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：10.12659/MSM.889023
更新日期：2013-07-22 00:00:00
abstract：:BACKGROUND Lymphocyte-to-monocyte ratio (LMR) is an independent predictive factor of clinical outcome of acute ischemic stroke and cancer, but the predictive effect of LMR in spontaneous intracerebral hemorrhage (ICH) is unknown. Thus, the aim of this study was to explore the impact of peripheral LMR on the neurologic...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.911645
更新日期：2018-12-20 00:00:00
abstract：BACKGROUND:Pre-conceptional folic acid supplementation is an effective way to reduce the incidence of neural tube defects (NTDs). Primary care providers are an important source of information to promote folic acid intake. This study aimed at evaluating primary care physicians' knowledge and attitudes regarding folic ac...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
abstract：:BACKGROUND To investigate the correlation between the relative computed tomography (CT) enhancement value and the microvascular architecture in different pathologic subtypes of renal cell carcinoma (RCC). MATERIAL AND METHODS This retrospective study included 55 patients with pathologically confirmed RCC. Immunohistoc...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.902957
更新日期：2017-07-31 00:00:00
abstract：BACKGROUND:Matrix metalloproteinase-2 (MMP-2) has been shown to activate a non-thromboxane, non-ADP pathway of platelet aggregation. In contrast, nitric oxide (NO) is known to inhibit platelet adhesion and aggregation. Therefore, we have studied the release of MMP-2 during platelet adhesion to fibrinogen, the effects o...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：BACKGROUND:Several studies have reported the association of PTPN2 gene with type 1 diabetes mellitus (T1DM) in many populations but not in the Chinese Han population. Therefore, the goal of our study was to replicate the reported association between 2 single-nucleotide polymorphisms (SNPs; rs478582 and rs2542151) in th...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.893607
更新日期：2015-09-07 00:00:00
abstract：:BACKGROUND Adenomyosis is a cause of chronic pelvic pain in women of reproductive age. The aim of this study was to investigate the effects of subcutaneous etonogestrel implantation on adenomyosis. MATERIAL AND METHODS A clinical observational study included 17 women with adenomyosis who were treated with subcutaneous...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.908979
更新日期：2018-09-01 00:00:00
abstract：BACKGROUND:The anti-oxidative and anti-inflammatory activities of electro-acupuncture (EA), a traditional clinical method, are widely accepted, but its mechanisms are not yet well defined. In this study, we investigated the role of extracellular signal-regulated kinases1/2 (ERK1/2) pathways on electro-acupuncture - med...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.890736
更新日期：2014-08-16 00:00:00
abstract：BACKGROUND:Haplotype analysis is hypothesized as having better power than individual SNPs in detecting the association between genotype and phenotype, but this approach has rarely been used in studies of the genetics of violent behavior in schizophrenia. The aim of this study was to further examine the role of the COMT...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2009-09-01 00:00:00
abstract：:BACKGROUND Increasing evidence suggests that delayed diagnosis in Crohn's disease is associated with a complicated disease course. The aim of this study was to explore the association between delayed diagnosis and the timing of the first Crohn's disease-related intestinal surgery. MATERIAL AND METHODS A retrospective ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.904238
更新日期：2017-10-09 00:00:00
abstract：:BACKGROUND Postextubation distress is detrimental to the prognosis of critically ill patients with successful spontaneous breathing trial. The known risk factors of failed weaning are associated with the heart, lungs, and diaphragm. The aim of this study was to explore the role of a combined model including indicators...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.924885
更新日期：2020-08-08 00:00:00
abstract：:BACKGROUND Lung cancer is one of the leading causes of mortality and morbidity. Viburnum grandiflorum is a medicinal herb known for its wide spectrum of pharmacological activities, but its anti-cancer properties against lung cancer cells have not been previously investigated. The present study elucidated the antitumor...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.920265
更新日期：2020-01-04 00:00:00
abstract：BACKGROUND:Interferon-gamma release assays have not been validated in active TB among pregnant women. Therefore, the objective of this retrospective study was to estimate the diagnostic value of T-SPOT.TB in active TB among pregnant women. MATERIAL/METHODS:Between May 2012 and May 2015, 26 consecutive pregnant women w...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.896943
更新日期：2016-01-06 00:00:00
abstract：BACKGROUND:Abdominally based perforator free flaps are commonly used in reconstructive breast surgery. Pre-operative assessment using a variety of imaging techniques has become increasingly popular to assess the anatomy of the abdominal perforators. We hypothesize that color flow duplex ultrasonography is a reliable me...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2010-08-01 00:00:00
abstract：:BACKGROUND The aim of this study was to investigate and compare the clinical efficacy of modular cementless stem and coated cementless long-stem prostheses in hip revision arthroplasty. MATERIAL AND METHODS Sixty-five patients with complete hip revision surgery data during January 2005 to March 2015 were selected from...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,随机对照试验
doi：10.12659/msm.895709
更新日期：2016-02-29 00:00:00
abstract：:BACKGROUND Nimotuzumab is an anti-EGFR monoclonal antibody which has been widely used in cancer treatment. However, the safety and efficacy of nimotuzumab combined with chemotherapy in locally advanced or metastatic esophageal cancer patients remain unclear. MATERIAL AND METHODS To address this open question, we colle...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,随机对照试验
doi：10.12659/msm.902645
更新日期：2017-01-24 00:00:00
abstract：BACKGROUND:Tracheal intubation of comatose patients is common, but contrary to most standards for respiratory care, heated nebulizers are not always used. This deviation from recommendations appears to be widespread. CASE REPORT:In the case examined, a tracheotomized patient suffering from severe anoxic brain injury w...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2006-08-01 00:00:00
abstract：:BACKGROUND Diabetes is a risk factor for coronary atherosclerosis and coronary heart disease. Resveratrol (RESV) is a natural compound with anti-inflammatory effects. The objective of this study is to evaluate the cardio protective effects of RESV in a diabetic rat model with coronary heart disease. MATERIAL AND METHO...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.910996
更新日期：2019-01-18 00:00:00
abstract：BACKGROUND:Inflammation and pulmonary injury caused by mechanical ventilation is a complex process in which long pentraxin 3 (PTX3) is suggested to play an important role. The effect of mechanical stretch on PTX3 mRNA and protein expression in human alveolar epithelial cells (A549) was investigated. MATERIAL/METHODS:I...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2009-05-01 00:00:00
abstract：:BACKGROUND Several studies have indicated that interleukin (IL)-1β-511 C/T polymorphism may contribute to individual susceptibility to gastric cancer, but the results vary among regions and races. No relevant meta-analysis has been conducted in a Chinese population. Therefore, we performed the current meta-analysis to...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,meta分析
doi：10.12659/msm.895771
更新日期：2016-05-24 00:00:00
abstract：BACKGROUND:The aim was to evaluate factors associated with the development of heart rupture in a Spanish registry of acute myocardial infarction (AMI) patients. MATERIAL/METHODS:This was a retrospective study of cohorts, including all patients diagnosed with AMI included in the ARIAM Spanish multicenter registry. The ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2010-05-01 00:00:00
abstract：BACKGROUND:Haemorrhagic hypotension is accompanied by an increase in endogenous opioid system activity, however, its significance is not fully recognised. Therefore, the role of endogenous opioid peptides in the central cardiovascular regulation in haemorrhagic shock was reviewed. CONCLUSIONS:Complex central opioid me...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2001-05-01 00:00:00
abstract：:BACKGROUND This study compared the efficacy and safety of 3 different anesthesia techniques used in total hip arthroplasty (THA). MATERIAL AND METHODS We allocated 198 patients preparing to undertake THA into 3 groups: general anesthesia group (GA group, n=66), caudal epidural anesthesia group (CEA group, n=66), and s...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 临床试验,杂志文章
doi：10.12659/msm.902768
更新日期：2017-08-02 00:00:00
abstract：BACKGROUND:Changing a central venous catheter occlusive dressing on a twice-weekly basis is usually recommended in hemato-oncological patients. A longer interval is believed to give rise to infections. However, frequent dressing changes might cause local cutaneous damage. MATERIAL/METHODS:Local cutaneous damage and in...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,多中心研究,随机对照试验
doi：
更新日期：2009-03-01 00:00:00
abstract：BACKGROUND:The prevalence of the deficiency of enzyme Lactase/phlorizin hydrolase (LPH) varies widely between different countries, with a low of 1-3% in Scandinavia and close to 100% in most of South-East Asia. Various carbohydrates are capable of enhancing LPH mRNA levels in the small intestine, and that transcription...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:BACKGROUND Abdominal bleeding is a severe complication of duodenal fistula, but few studies have focused on this problem. The purpose of the present study was to investigate the prevalence of and risk factors for intra-abdominal bleeding in patients with external duodenal fistula. MATERIAL AND METHODS From January 201...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.912651
更新日期：2018-12-21 00:00:00
abstract：:BACKGROUND Patient-specific instruments have recently gained attention for ensuring the accuracy of osteotomy and correct alignment in total knee arthroplasty (TKA). We aimed to report our experience with TKA performed by using CT-based 3D model of cutting guides in our clinic. MATERIAL AND METHODS A total of 36 patie...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.908213
更新日期：2017-12-29 00:00:00
abstract：:BACKGROUND Radiotherapy is explicitly indicated as one of the excluding factors in diagnosing overactive bladder syndrome (OAB). Nevertheless, symptoms of OAB such as urgent episodes, incontinence, pollakiuria, and nocturia, which are consequences of irradiation, led us to test the effectiveness of VESIcare®/Solifenac...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.899327
更新日期：2016-07-30 00:00:00
