Abstract：
BACKGROUND:Matrix metalloproteinase-2 (MMP-2) has been shown to activate a non-thromboxane, non-ADP pathway of platelet aggregation. In contrast, nitric oxide (NO) is known to inhibit platelet adhesion and aggregation. Therefore, we have studied the release of MMP-2 during platelet adhesion to fibrinogen, the effects of phenanthrolione, an MMP-2 inhibitor, on adhesion and the interactions of inhibitor with a NO donor, S-nitroso-N-acetyl-D, L-penicillamine (SNAP). MATERIAL AND METHODS:Human platelets were isolated from blood of healthy volunteers and platelet adhesion to fibrinogen-coated plates was studied by measuring thrombin-stimulated release of platelet a-granule constituent, platelet factor 4. In addition, the mode of action of phenanthroline and NO on platelets was investigated by assaying the levels of intraplatelet cyclic GMP. RESULTS:Thrombin-stimulated platelet adhesion to fibrinogen was associated with increased release of MMP-2 from platelets. Phenanthroline (0.1-100 KM) reduced platelet adhesion to fibrinogen. The adhesion was also inhibited by SNAP (0.1-100 KM), an effect abolished by 1H-[1,2,4] oxadiazolol [4,3,-a] quinoxalin-1-one (ODQ), a selective inhibitor of the soluble guanylate cyclase. Co-administration of phenanthroline and SNAP resulted in a synergistic inhibition of platelet adhesion, an effect that was not associated with enhanced cyclic GMP generation by platelets. Furthermore, ODQ did not reverse the synergistic effect of these compounds on adhesion. CONCLUSIONS:1. MMP-2 promotes platelet adhesion to fibrinogen. 2. Phenanthroline and NO synergize to inhibit platelet adhesion to fibrinogen acting through a cyclic GMP-independent mechanism(s).
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Martinez A,Salas E,Radomski A,Radomski MWsubject
Has Abstractpub_date
2001-07-01 00:00:00pages
646-51issue
4eissn
1234-1010issn
1643-3750pii
2059journal_volume
7pub_type
杂志文章abstract：:BACKGROUND Emerging evidence suggests the involvement of Reelin in chemoresistance in various cancers. However, its function in cisplatin (DDP) sensitivity of non-small cell lung cancer (NSCLC) needs to be investigated. MATERIAL AND METHODS Reelin expression in cisplatin-sensitive A549 cells and cisplatin-resistant NS...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.925298
更新日期：2020-08-07 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.897542
更新日期：2017-08-17 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.919422
更新日期：2020-02-16 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.911536
更新日期：2018-11-09 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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更新日期：2017-10-17 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2018-12-17 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2002-04-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2018-07-18 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.882516
更新日期：2012-03-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2015-07-07 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2019-11-23 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.922129
更新日期：2020-06-07 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2019-07-08 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2018-05-26 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2017-10-19 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2018-01-18 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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更新日期：2019-07-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2017-05-16 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.883261
更新日期：2012-08-01 00:00:00
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abstract：:BACKGROUND Lower limb and pelvic injuries and fractures occur at a very high incidence in motor vehicle accidents. In this study, the characteristics (e.g., body side, bone location, and fracture severity) of lower limb and pelvic fractures that occurred during front-impact collisions were correlated with the injured ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2016-12-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
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更新日期：2011-05-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2020-09-12 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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更新日期：2018-03-31 00:00:00
abstract：BACKGROUND:The purpose of our study was to investigate both qualitatively and quantitatively the microbial content of probiotic products licensed for medicinal purposes. MATERIAL/METHODS:Microbiological analysis was performed on five different brands of probiotic products that claimed to contain lactobacilli and/or bi...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
