当前位置： SCI文献检索 > Molecular Oncology期刊下所有文献 > Subjecting appropriate lung adenocarcinoma samples to next-generation sequencing-based molecular testing: challenges and possible solutions.

Subjecting appropriate lung adenocarcinoma samples to next-generation sequencing-based molecular testing: challenges and possible solutions.

Abstract：

:Next-generation sequencing (NGS) has recently been rapidly adopted in the molecular diagnosis of cancer, but it still faces some obstacles. In this study, 665 lung adenocarcinoma samples (558 TKI-naive and 107 TKI-relapsed samples) were interrogated using NGS, and the challenges and possible solutions of subjecting appropriate tissue samples to NGS testing were explored. The results showed that lower frequencies of HER2/BRAF/PIK3CA and acquired EGFR T790M mutations were observed in biopsy samples with <20% tumor cellularity than in those with ≥20%, but there were no significant differences in the frequencies of EGFR or KRAS mutations. Moreover, tumor heterogeneity was assessed by heterogeneity score (HS), which was calculated through multiplying by 2 the mutant allele frequency (MAF) of tumor cells. In TKI-naive samples, intratumor heterogeneity could occur in EGFR, KRAS, HER2, BRAF, and PIK3CA mutant tumors, but the degree was variable. Higher EGFR, but lower BRAF and PIK3CA HS values were observed compared with KRAS HS. In TKI-relapsed samples, analysis of concomitant sensitizing EGFR and T790M MAFs showed that intratumor heterogeneity was common in acquired EGFR T790M mutant tumors. The mutational status between primary and metastatic tumors was usually concordant, but KRAS, HER2, and PIK3CA HS were significantly higher in metastatic tumors than in primary tumors. Additionally, the discordance rate of mutational status in multifocal lung adenocarcinomas diagnosed as equivocal or multiple primary tumors was high. Together, our findings demonstrate that a comprehensive quality assessment is necessary during tissue process to mitigate the challenges of poor tumor cellularity, tumor heterogeneity, and multifocal clonally independent tumors.

journal_name

Mol Oncol

journal_title

Molecular oncology

authors

Li W,Qiu T,Ling Y,Gao S,Ying J

doi

10.1002/1878-0261.12190

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

677-689

issue

5

eissn

1574-7891

issn

1878-0261

journal_volume

12

pub_type

杂志文章

相关文献

Molecular Oncology文献大全
  • miR-634 exhibits anti-tumor activities toward hepatocellular carcinoma via Rab1A and DHX33.

    abstract：:Deregulation of microRNAs contributes to the aberrant growth of hepatocellular carcinoma (HCC). Here, we showed that miR-634 expression was frequently decreased in HCC. Low miR-634 expression was significantly associated with larger tumor size, poorer tumor differentiation, advanced TNM stage, vascular invasion, absen...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1016/j.molonc.2016.09.001

    authors： Zhang CZ,Cao Y,Fu J,Yun JP,Zhang MF

    更新日期：2016-12-01 00:00:00

  • Translational cancer research - a coherent cancer research continuum.

    abstract：:The main components of the cancer research continuum are basic/preclinical research, early and late clinical research and, after the adoption of an innovation by the healthcare or health organisations, outcomes research. Translational cancer research, defined as a coherent cancer research continuum, is mandatory to ad...

    journal_title：Molecular oncology

    pub_type： 杂志文章,评审

    doi：10.1002/1878-0261.12450

    authors： Ringborg U

    更新日期：2019-03-01 00:00:00

  • Epigenetic alterations involved in cancer stem cell reprogramming.

    abstract：:Current hypotheses suggest that tumors originate from cells that carry out a process of "malignant reprogramming" driven by genetic and epigenetic alterations. Multiples studies reported the existence of stem-cell-like cells that acquire the ability to self-renew and are able to generate the bulk of more differentiate...

    journal_title：Molecular oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molonc.2012.10.006

    authors： Muñoz P,Iliou MS,Esteller M

    更新日期：2012-12-01 00:00:00

  • Essential role of HDAC6 in the regulation of PD-L1 in melanoma.

    abstract：:Histone deacetylases (HDACs), originally described as histone modifiers, have more recently been demonstrated to target a variety of other proteins unrelated to the chromatin environment. In this context, our present work demonstrates that the pharmacological or genetic abrogation of HDAC6 in primary melanoma samples ...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1016/j.molonc.2015.12.012

    authors： M L,P PV,T K,M P,E S,J P,K V W,C L,F C,S D,M SKS,M M,A K,J PI,A S,E S,J W,E M S,A V

    更新日期：2016-05-01 00:00:00

  • The noncoding function of NELFA mRNA promotes the development of oesophageal squamous cell carcinoma by regulating the Rad17-RFC2-5 complex.

    abstract：:Recently, RNAs interacting with proteins have been implicated in playing an important role in the occurrence and progression of oesophageal squamous cell carcinoma (ESCC). In this study, we found that NELFA mRNA interacts with Rad17 through a novel noncoding mode in the nucleus and that the aberrant expression of USF2...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12619

    authors： Xu J,Wang G,Gong W,Guo S,Li D,Zhan Q

    更新日期：2020-03-01 00:00:00

  • CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas.

    abstract：:The elevated expression and activation of human telomerase reverse transcriptase (hTERT) is associated with the unlimited proliferation of cancer cells. However, the excise mechanism of hTERT regulation during carcinogenesis is not well understood. In this study, we discovered cleavage and polyadenylation specific fac...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1016/j.molonc.2014.02.001

    authors： Chen W,Qin L,Wang S,Li M,Shi D,Tian Y,Wang J,Fu L,Li Z,Guo W,Yu W,Yuan Y,Kang T,Huang W,Deng W

    更新日期：2014-05-01 00:00:00

  • Detection of circulating tumor cells in colorectal cancer patients using the GILUPI CellCollector: results from a prospective, single-center study.

    abstract：:The GILUPI CellCollector (CC) is a novel in vivo circulating tumor cell (CTC) detection device reported to overcome the limitations of small blood sample volumes. The aim of this prospective, blinded study was to evaluate the clinical application of the CC and to compare its performance to the CellSearch (CS) system i...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12507

    authors： Dizdar L,Fluegen G,van Dalum G,Honisch E,Neves RP,Niederacher D,Neubauer H,Fehm T,Rehders A,Krieg A,Knoefel WT,Stoecklein NH

    更新日期：2019-07-01 00:00:00

  • Dual inhibition of Wnt and Yes-associated protein signaling retards the growth of triple-negative breast cancer in both mesenchymal and epithelial states.

    abstract：:Triple-negative breast cancer (TNBC), the most refractory subtype of breast cancer to current treatments, accounts disproportionately for the majority of breast cancer-related deaths. This is largely due to cancer plasticity and the development of cancer stem cells (CSCs). Recently, distinct yet interconvertible mesen...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12167

    authors： Sulaiman A,McGarry S,Li L,Jia D,Ooi S,Addison C,Dimitroulakos J,Arnaout A,Nessim C,Yao Z,Ji G,Song H,Gadde S,Li X,Wang L

    更新日期：2018-04-01 00:00:00

  • Dysregulation of histone methyltransferases in breast cancer - Opportunities for new targeted therapies?

    abstract：:Histone methyltransferases (HMTs) catalyze the methylation of lysine and arginine residues on histone tails and non-histone targets. These important post-translational modifications are exquisitely regulated and affect chromatin compaction and transcriptional programs leading to diverse biological outcomes. There is a...

    journal_title：Molecular oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molonc.2016.09.003

    authors： Michalak EM,Visvader JE

    更新日期：2016-12-01 00:00:00

  • Tackling the cancer burden: the economic impact of primary prevention policies.

    abstract：:Cancer is a noncommunicable disease (NCD) with increasing incidence and therefore constitutes a major public health issue. To reduce the health and economic burden of cancer, policy-makers across the world have implemented a range of preventative interventions targeting risk factors with a known link to the disease. I...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12812

    authors： Cheatley J,Aldea A,Lerouge A,Devaux M,Vuik S,Cecchini M

    更新日期：2020-10-05 00:00:00

  • Cell-to-cell communication: microRNAs as hormones.

    abstract：:Mammalian cells can release different types of extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies. Accumulating evidence suggests that EVs play a role in cell-to-cell communication within the tumor microenvironment. EVs' components, such as proteins, noncoding RNAs [microRNAs (miRNAs...

    journal_title：Molecular oncology

    pub_type： 杂志文章,评审

    doi：10.1002/1878-0261.12144

    authors： Bayraktar R,Van Roosbroeck K,Calin GA

    更新日期：2017-12-01 00:00:00

  • Interaction of transcription factor AP-2 gamma with proto-oncogene PELP1 promotes tumorigenesis by enhancing RET signaling.

    abstract：:A significant proportion of estrogen receptor-positive (ER+) breast cancer (BC) initially responds to endocrine therapy but eventually evolves into therapy-resistant BC. Transcription factor AP-2 gamma (TFAP2C) is a known regulator of ER activity, and high expression of TFAP2C is associated with a decreased response t...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12871

    authors： Liu J,Liu Z,Li M,Tang W,Pratap UP,Luo Y,Altwegg KA,Li X,Zou Y,Zhu H,Sareddy GR,Viswanadhapalli S,Vadlamudi RK

    更新日期：2020-12-02 00:00:00

  • A 17 gene panel for non-small-cell lung cancer prognosis identified through integrative epigenomic-transcriptomic analyses of hypoxia-induced epithelial-mesenchymal transition.

    abstract：:As a critical feature of the tumor microenvironment, hypoxia is known to be a potent inducer of tumor metastasis, and it has been proposed that the initial steps in metastasis involve epithelial-mesenchymal transition (EMT). The strong correlation among hypoxia, EMT, and metastasis suggests that integrative assessment...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12491

    authors： Chen YL,Zhang Y,Wang J,Chen N,Fang W,Zhong J,Liu Y,Qin R,Yu X,Sun Z,Gao F

    更新日期：2019-07-01 00:00:00

  • Methylation-associated miR-193b silencing activates master drivers of aggressive prostate cancer.

    abstract：:Epigenetic silencing of miRNA is a primary mechanism of aberrant miRNA expression in cancer, and hypermethylation of miRNA promoters has been reported to contribute to prostate cancer initiation and progression. Recent data have shown that the miR-193b promoter is hypermethylated in prostate cancer compared with norma...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12536

    authors： Mazzu YZ,Yoshikawa Y,Nandakumar S,Chakraborty G,Armenia J,Jehane LE,Lee GM,Kantoff PW

    更新日期：2019-09-01 00:00:00

  • Diphenhydramine increases the therapeutic window for platinum drugs by simultaneously sensitizing tumor cells and protecting normal cells.

    abstract：:Platinum-based compounds remain a well-established chemotherapy for cancer treatment despite their adverse effects which substantially restrict the therapeutic windows of the drugs. Both the cell type-specific toxicity and the clinical responsiveness of tumors have been associated with mechanisms that alter drug entry...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12648

    authors： Melnikova M,Wauer US,Mendus D,Hilger RA,Oliver TG,Mercer K,Gohlke BO,Erdmann K,Niederacher D,Neubauer H,Buderath P,Wimberger P,Kuhlmann JD,Thomale J

    更新日期：2020-04-01 00:00:00

  • The metastasis-associated molecule C4.4A promotes tissue invasion and anchorage independence by associating with the alpha6beta4 integrin.

    abstract：:C4.4A is a metastasis-associated molecule that functions appear to rely on associated alph6beta4 integrin. To corroborate the impact of the C4.4A-alpha6beta4 integrin association on metastasis formation, C4.4A was knocked-down in a highly metastatic rat pancreatic adenocarcinoma (ASML, ASML-C4.4Akd). Metastasis format...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1016/j.molonc.2013.05.002

    authors： Thuma F,Ngora H,Zöller M

    更新日期：2013-10-01 00:00:00

  • Screening and identification of small molecule inhibitors of ErbB2-induced invasion.

    abstract：:ERBB2 amplification and overexpression are strongly associated with invasive cancer with high recurrence and poor prognosis. Enhanced ErbB2 signaling induces cysteine cathepsin B and L expression leading to their higher proteolytic activity (zFRase activity), which is crucial for the invasion of ErbB2-positive breast ...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1016/j.molonc.2014.07.004

    authors： Brix DM,Rafn B,Bundgaard Clemmensen K,Andersen SH,Ambartsumian N,Jäättelä M,Kallunki T

    更新日期：2014-12-01 00:00:00

  • Theranostic applications of antibodies in oncology.

    abstract：:Targeted therapies, including antibodies, are becoming increasingly important in cancer therapy. Important limitations, however, are that not every patient benefits from a specific antibody therapy and that responses could be short-lived due to acquired resistance. In addition, targeted therapies are quite expensive a...

    journal_title：Molecular oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molonc.2014.03.010

    authors： Fleuren ED,Versleijen-Jonkers YM,Heskamp S,van Herpen CM,Oyen WJ,van der Graaf WT,Boerman OC

    更新日期：2014-06-01 00:00:00

  • Gene regulatory networking reveals the molecular cue to lysophosphatidic acid-induced metabolic adaptations in ovarian cancer cells.

    abstract：:Extravasation and metastatic progression are two main reasons for the high mortality rate associated with cancer. The metastatic potential of cancer cells depends on a plethora of metabolic challenges prevailing within the tumor microenvironment. To achieve higher rates of proliferation, cancer cells reprogram their m...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12046

    authors： Ray U,Roy Chowdhury S,Vasudevan M,Bankar K,Roychoudhury S,Roy SS

    更新日期：2017-05-01 00:00:00

  • Genetic susceptibility to breast cancer.

    abstract：:Genetic and lifestyle/environmental factors are implicated in the aetiology of breast cancer. This review summarizes the current state of knowledge on rare high penetrance mutations, as well as moderate and low-penetrance genetic variants implicated in breast cancer aetiology. We summarize recent discoveries from larg...

    journal_title：Molecular oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molonc.2010.04.011

    authors： Mavaddat N,Antoniou AC,Easton DF,Garcia-Closas M

    更新日期：2010-06-01 00:00:00

  • Post-transcriptional regulation of PIAS3 expression by miR-18a in malignant mesothelioma.

    abstract：:Protein inhibitor of activated STAT3 (PIAS3) is an endogenous suppressor of signal transducer and activator of transcription 3 (STAT3) signaling. By directly interacting with phosphorylated STAT3, PIAS3 can block the downstream transcriptional activity of STAT3, which is hyper-activated in various cancers. We previous...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12386

    authors： He T,McColl K,Sakre N,Chen Y,Wildey G,Dowlati A

    更新日期：2018-12-01 00:00:00

  • Dying tumor cells stimulate proliferation of living tumor cells via caspase-dependent protein kinase Cδ activation in pancreatic ductal adenocarcinoma.

    abstract：:Pancreatic cancer is one of the most lethal human cancers, and radiotherapy is often implemented for locally advanced pancreatic ductal adenocarcinoma. Tumor cell repopulation is a major challenge in treating cancers after radiotherapy. In order to address the problem of tumor repopulation, our previous studies have d...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1016/j.molonc.2014.07.024

    authors： Cheng J,Tian L,Ma J,Gong Y,Zhang Z,Chen Z,Xu B,Xiong H,Li C,Huang Q

    更新日期：2015-01-01 00:00:00

  • Comparison of frequencies and prognostic effect of molecular subtypes between young and elderly breast cancer patients.

    abstract：PURPOSE:To compare the distribution and prognostic effect of the breast cancer molecular subtypes in young and elderly breast cancer patients. PATIENTS AND METHODS:Our study population (n = 822) consisted of all early breast cancer patients primarily treated with surgery in our center between 1985 and 1996. A total of...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1016/j.molonc.2014.03.022

    authors： de Kruijf EM,Bastiaannet E,Rubertá F,de Craen AJ,Kuppen PJ,Smit VT,van de Velde CJ,Liefers GJ

    更新日期：2014-07-01 00:00:00

  • Activation of TNF-α/NF-κB axis enhances CRL4BDCAF11 E3 ligase activity and regulates cell cycle progression in human osteosarcoma cells.

    abstract：:Cullin 4B, a member of the Cullins, which serve as scaffolds to facilitate the assembly of E3 ligase complexes, is aberrantly expressed in many cancers, including osteosarcoma. Recently, we observed that CUL4B forms the CRL4BDCAF11 E3 ligase, which specifically ubiquitinates and degrades the cyclin-dependent kinase (C...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12176

    authors： Zhang C,Chen B,Jiang K,Lao L,Shen H,Chen Z

    更新日期：2018-04-01 00:00:00

  • Inhibition of PARP1-dependent end-joining contributes to Olaparib-mediated radiosensitization in tumor cells.

    abstract：:Poly-ADP-ribose-polymerase inhibitors (PARPi) are considered to be optimal tools for specifically enhancing radiosensitivity. This effect has been shown to be replication-dependent and more profound in HR-deficient tumors. Here, we present a new mode of PARPi-mediated radiosensitization which was observed in four out ...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1016/j.molonc.2014.06.008

    authors： Kötter A,Cornils K,Borgmann K,Dahm-Daphi J,Petersen C,Dikomey E,Mansour WY

    更新日期：2014-12-01 00:00:00

  • Clinical implications of plasma circulating tumor DNA in gynecologic cancer patients.

    abstract：:Molecular characterization of cancers is important in dictating prognostic factors and directing therapy. Next-generation sequencing of plasma circulating tumor DNA (ctDNA) offers less invasive, more convenient collection, and a more real-time representation of a tumor and its molecular heterogeneity than tissue. Howe...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12791

    authors： Charo LM,Eskander RN,Okamura R,Patel SP,Nikanjam M,Lanman RB,Piccioni DE,Kato S,McHale MT,Kurzrock R

    更新日期：2021-01-01 00:00:00

  • Profiling of microRNAs in tumor interstitial fluid of breast tumors - a novel resource to identify biomarkers for prognostic classification and detection of cancer.

    abstract：:It has been hypothesized based on accumulated data that a class of small noncoding RNAs, termed microRNAs, are key factors in intercellular communication. Here, microRNAs present in interstitial breast tumor fluids have been analyzed to identify relevant markers for a diagnosis of breast cancer and to elucidate the cr...

    journal_title：Molecular oncology

    pub_type： 临床试验,杂志文章

    doi：10.1002/1878-0261.12025

    authors： Halvorsen AR,Helland Å,Gromov P,Wielenga VT,Talman MM,Brunner N,Sandhu V,Børresen-Dale AL,Gromova I,Haakensen VD

    更新日期：2017-02-01 00:00:00

  • FOXM1D Potentiates PKM2-mediated tumor glycolysis and angiogenesis.

    abstract：:Tumor growth, especially in the late stage, requires adequate nutrients and rich vasculature, in which PKM2 plays a convergent role. It has been reported that PKM2, together with FOXM1D, is upregulated in late stage colorectal cancer and associated with metastasis; however, their underlying mechanism for promoting tum...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12879

    authors： Zhang W,Zhang X,Huang S,Chen J,Ding P,Wang Q,Li L,Lv X,Li L,Zhang P,Zhou D,Wen W,Wang Y,Lei QY,Wu J,Hu W

    更新日期：2020-12-13 00:00:00

  • NFIB is a potential target for estrogen receptor-negative breast cancers.

    abstract：BACKGROUND:The association between nuclear factor I/B (NFIB) gene and triple negative breast cancer has been previously suggested. METHODS:We investigated the relationship between NFIB mRNA and protein expression and molecular subtypes of breast cancer as well as the effect of NFIB silencing on the proliferation and a...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1016/j.molonc.2011.08.002

    authors： Moon HG,Hwang KT,Kim JA,Kim HS,Lee MJ,Jung EM,Ko E,Han W,Noh DY

    更新日期：2011-12-01 00:00:00

  • Programmed death-ligand 1 gene expression is a prognostic marker in early breast cancer and provides additional prognostic value to 21-gene and 70-gene signatures in estrogen receptor-positive disease.

    abstract：:Gene and protein expression of programmed death-ligand 1 (PD-L1) are prognostic in early breast cancer (BC), but their prognostic information is inconsistent at least in some biological subgroups. The validated prognostic gene signatures (GS) in BC are mainly based on proliferation and estrogen receptor (ER)-related g...

    journal_title：Molecular oncology

    pub_type： 杂志文章

    doi：10.1002/1878-0261.12654

    authors： Zerdes I,Sifakis EG,Matikas A,Chrétien S,Tobin NP,Hartman J,Rassidakis GZ,Bergh J,Foukakis T

    更新日期：2020-05-01 00:00:00