Abstract:
BACKGROUND:We have developed a rat cell model for studying collagen type I production in coronary artery adventitial fibroblasts. Increased deposition of adventitial collagen type I leads to stiffening of the blood vessel, increased blood pressure, arteriosclerosis and coronary heart disease. Although the source and mechanism of collagen deposition is yet unknown, the adventitia appears to play a significant role. To demonstrate the application of our cell model, cultured adventitial fibroblasts were treated with sex hormones and the effect on collagen production measured. METHODS:Hearts (10-12 weeks) were harvested and the left anterior descending coronary artery (LAD) was isolated and removed. Tissue explants were cultured and cells (passages 2-4) were confirmed as fibroblasts using immunohistochemistry. Optimal conditions were determined for cell tissue harvest, timing, proliferation and culture conditions. Fibroblasts were exposed to 10-7 M testosterone or 10-7 M estrogen for 24 hours and either immunostained for collagen type I or subjected to ELISA. RESULTS:Results showed increased collagen staining in fibroblasts treated with testosterone compared to control and decreased staining with estrogen. ELISA results showed that testosterone increased collagen I by 20% whereas estrogen decreased collagen I by 15%. CONCLUSION:Data demonstrates the usefulness of our cell model in studying the specific role of the adventitia apart from other blood vessel tissue in rat coronary arteries. Results suggest opposite effects of testosterone and estrogen on collagen synthesis in the rat coronary artery adventitial fibroblasts.
journal_name
BMC Cardiovasc Disordjournal_title
BMC cardiovascular disordersauthors
Jenkins C,Milsted A,Doane K,Meszaros G,Toot J,Ely Ddoi
10.1186/1471-2261-7-13subject
Has Abstractpub_date
2007-05-08 00:00:00pages
13issn
1471-2261pii
1471-2261-7-13journal_volume
7pub_type
杂志文章abstract:BACKGROUND:Ectonucleotidase plays an important role in the regulation of cardiac function by controlling extracellular levels of adenine nucleotides and adenosine. To determine the influence of ischemia-reperfusion injury on ectonucleotidase activity in coronary vascular bed, we compared the metabolic profile of adenin...
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章,meta分析
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