Abstract:
BACKGROUND:Acetylcholine and bradykinin cause endothelium-dependent hyperpolarization and relaxation of vascular smooth muscle. These responses are mediated in part by an endothelium-derived hyperpolarizing factor (EDHF). Epoxyeicosatrienoic acids (EETs) are cytochrome P450 metabolites of arachidonic acid and appear to function as EDHFs in the coronary artery. This conclusion is based on the findings that EETs are synthesized by the vascular endothelium, open calcium-activated potassium (KCa) channels, hyperpolarize and relax vascular smooth muscle. METHODS:Bovine coronary arteries were precontracted with U46619 and isometric tension was measured to agonists. Whole cell K currents were measured in coronary smooth muscle cells by the patch clamp method. RESULTS:Bradykinin caused a concentration-related relaxation (ED50=10(-10) M) which was not affected by inhibitors of cytochrome P450. Inhibition of NO synthase and cyclooxygenase reduced the response to bradykinin (ED(50)=2 x 10(-9) M) and these responses were then completely blocked by cytochrome P450 inhibitors. 11, 12-EET relaxed the precontracted bovine coronary artery with an ED50 of 3 x 10(-8) M in the presence and absence of the endothelium. 14, 15-EET caused an increase in outward potassium current that was blocked by the KCa channel inhibitor iberiotoxin. A series of 14, 15-EET analogs were tested for agonist activity. CONCLUSIONS:These data indicate that cytochrome P450 metabolites contribute to bradykinin-induced, endothelium-dependent relaxations but only following inhibition of NO synthase and cyclooxygenase. EETs relax coronary arteries in an endothelium-independent manner. Carbon-1 carboxyl, 8, 9 and 11, 12-double bonds and 14, 15-epoxy groups are required for full agonist activity. These studies provide further support for the hypothesis that EETs represent EDHFs in the coronary artery and mediate the relaxation responses to bradykinin.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Campbell WB,Falck JR,Gauthier Ksubject
Has Abstractpub_date
2001-07-01 00:00:00pages
578-84issue
4eissn
1234-1010issn
1643-3750pii
2018journal_volume
7pub_type
杂志文章abstract::Background Non-alcoholic fatty liver disease is considered a hepatic manifestation of metabolic syndrome. Periodontal disease is a mild chronic inflammatory disease with systemic effects, and many studies have indicated an association between metabolic syndrome and periodontitis. In the present study, we investigated ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.891204
更新日期:2014-10-31 00:00:00
abstract:BACKGROUND:Little is known about the effects of low-frequency repetitive transcranial magnetic stimulation (rTMS) on dysmnesia and the impact of brain nucleotide neurotrophic factor (BDNF) Val66Met single-nucleotide polymorphism (SNP). This study investigated the impact of low-frequency rTMS on post-stroke dysmnesia an...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,随机对照试验
doi:10.12659/MSM.892337
更新日期:2015-03-14 00:00:00
abstract:BACKGROUND:To evaluate the pain, quality of life (QOL), and limb function of patients after en bloc resection of solitary metastatic bone cancer in the limbs. MATERIAL/METHODS:A total of 27 patients with solitary metastatic bone cancer in the limbs were recruited. All these patients underwent limb-salvage surgery with...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 临床试验,杂志文章
doi:10.12659/msm.883542
更新日期:2012-11-01 00:00:00
abstract::BACKGROUND Nasal obstruction is the most common rhinologic complaint in ear, nose, and throat (ENT) clinical practice and septal deviation is the leading cause. The Nasal Obstruction Symptom Evaluation (NOSE) scale is a brief, self-administered questionnaire that has been widely used to assess symptoms and quality of ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.909934
更新日期:2018-11-06 00:00:00
abstract::BACKGROUND Er-Miao-San (EMS) is used in traditional Chinese medicine. This study aimed to investigate the effect of different elution fractions of EMS on acute inflammation induced by carrageenan in the rat paw and the possible mechanisms of action. MATERIAL AND METHODS Different aqueous fractions of EMS added to an A...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.916977
更新日期:2019-10-23 00:00:00
abstract::BACKGROUND Sepsis causes the highest mortality in non-cardiovascular intensive care units worldwide. Recent research has demonstrated that the late phase of sepsis, characterized as septic immunosuppression, is the central pathophysiological mechanism of immune dysfunction. Investigating the suppressive mechanism of i...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.908242
更新日期:2018-07-11 00:00:00
abstract:BACKGROUND:The eradication of Helicobacter pylori (HP) is dependent on several bacteriologic and geographic variables. Multiple-drug regimens have been tried in order to eradicate HPi; however, the optimal drug has not yet been found. MATERIAL/METHODS:In this single-blind, randomized clinical trial, 245 dyspeptic pati...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2005-04-01 00:00:00
abstract::BACKGROUND Phenylketonuria (PKU) is an inborn error of metabolism caused by mutations in the phenylalanine hydroxylase (PAH) gene. When untreated, PKU leads to a significant intellectual deficiency. Although early initiation of dietary therapy allows normal cognitive development, low adherence to treatment may result ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.909146
更新日期:2018-10-30 00:00:00
abstract::BACKGROUND From the end of December 2019, coronavirus disease 2019 (COVID-19) began to spread in central China. Social capital is a measure of social trust, belonging, and participation. This study aimed to investigate the effects of social capital on sleep quality and the mechanisms involved in people who self-isolat...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.923921
更新日期:2020-03-20 00:00:00
abstract:BACKGROUND:There are many systemic complications of conventional selective renal arteriography (SRA), such as contrast-mediated nephropathy. Contrast-enhanced magnetic resonance angiography (CE-MRA) and renal artery Doppler ultrasonography (DUSG) have been used increasingly for renal artery stenosis (RAS). The aim of t...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.889035
更新日期:2013-06-19 00:00:00
abstract:BACKGROUND:HealthCyberMap (http://healthcybermap.semanticweb.org/) aims at mapping Internet health information resources in novel ways for enhanced retrieval and navigation. This is achieved by collecting appropriate resource metadata in an unambiguous form that preserves semantics. MATERIAL/METHODS:We modelled a qual...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract:BACKGROUND:N-epsilon-(Carboxymethyl)Lysine (CML), a major advanced glycation end product, is expressed in the lower respiratory tract. In this study, we compared the validity of measuring CML levels and percentage of eosinophils in induced sputum for assessment of airway functions, and evaluated the clinical implicatio...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 临床试验,杂志文章
doi:
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND:The aim of this study was to determine the relevance of apelin and insulin resistance (IR) with polycystic ovary syndrome (PCOS) and to assess the possible therapeutic effect of the combined therapy of drospirenone-ethinylestradiol (DRSP-EE) combined with metformin. MATERIAL AND METHODS:Sixty-three PCOS pat...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.894926
更新日期:2015-08-28 00:00:00
abstract:BACKGROUND:Macrolide antibiotics are highly active in vitro against B.burgdorferi, but have limited efficacy in the treatment of patients with Lyme Disease. As macrolides are less active at a low pH, their poor clinical activity might be due to localization of borrelia to an acidic endosome, and their activity improved...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::Osterix (Osx) is an osteoblast-specific transcription factor that is essential for bone formation. MicroRNAs (miRNAs) are ~22-nucleotide-long noncoding RNAs that play important regulatory roles in animals and plants by targeting mRNAs for cleavage or translational repression. They can also control osteoblast-mediated ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,评审
doi:10.12659/msm.896742
更新日期:2016-08-20 00:00:00
abstract:BACKGROUND:After left ventricular assist device (LVAD) implantation, post-operative renal failure is a fairly high risk. While comparing the differences in indicators of renal function associated with the use of three different types of LVAD, this retrospective study was designed to evaluate predictors and outcomes of ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2008-12-01 00:00:00
abstract:BACKGROUND:Prophylaxis of toxoplasmic encephalitis was performed with pyrimethamine in 6 patients with advanced HIV infection during combination therapy with protease inhibitors. MATERIAL/METHODS:Steady-state plasma pyrimethamine (PYR) levels were measured by gas chromatography. Protease inhibitor plasma concentration...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND:Water immersion stress (WIS) in rats causes decrease in deformability of red blood cells (RBC). We hypothesize that this phenomenon is mediated by reactive oxygen species. Since tirilazad mesylate (U74006F) is a potent scavenger of free oxygen radicals, the aim of this study was to investigate possible influ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::BACKGROUND Cardiac infarction frequently leads to arrhythmia and ischemia/reperfusion (I/R) aggravates cardiac injury. Pinocembrin can resist cerebral ischemia and decrease cardiac infarction area. This study thus generated a rat myocardial I/R model to assess the effect on ventricular rhythm and expression of gap jun...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.909162
更新日期:2018-07-19 00:00:00
abstract::Background Intracranial arterial variations are a frequent finding in the general population. Knowledge of these vascular variations has significant clinical impact because some of them predispose patients to development of an aneurysm or cerebrovascular ischemic disease. The purpose of this study was to evaluate the ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.890265
更新日期:2014-03-14 00:00:00
abstract::BACKGROUND Acute exacerbation of chronic obstructive pulmonary disease (AECOPD) aggravates the overall severity in COPD patients, resulting in severe morbidity and mortality. However, there are no objective biomarkers currently available to predict the development of AECOPD. Several studies have indicated that galecti...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.903472
更新日期:2017-09-26 00:00:00
abstract::BACKGROUND Gastric cancer (GC) is the third leading cause of cancer-associated mortality in the world. Expression of circular RNA circ_C16orf62 is reported to be low in GC. The role and mechanism of circ_C16orf62 remain unclear. MATERIAL AND METHODS Expression levels of circ_C16orf62 and tubulin beta-2A chain (TUBB2A)...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.924343
更新日期:2020-10-02 00:00:00
abstract:BACKGROUND:The ideal surgical approach for cervical disk disease remains controversial, especially for multilevel cervical disease. The purpose of this study was to investigate the biomechanics of the cervical spine after 3-level hybrid surgery compared with 3-level anterior cervical discectomy and fusion (ACDF). MATE...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.896085
更新日期:2015-11-03 00:00:00
abstract::BACKGROUND This study aimed to assess the roles of the Morris index in predicting poor outcomes in patients with non-ST segment elevation acute coronary syndrome (NSTE-ACS). MATERIAL AND METHODS This study included 905 patients with newly diagnosed NSTE-ACS. The Morris index, also known as P wave terminal force in lea...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.924418
更新日期:2020-10-19 00:00:00
abstract::BACKGROUND The ADRB2 gene encodes the ß2-adrenergic receptor (ß2-AR). This study aimed to determine the association between the C79G polymorphism of the ADRB2 gene and its association with pediatric asthma using a meta-analysis of the published data. MATERIAL AND METHODS Review of publications up to May 2018 was from ...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,meta分析,评审
doi:10.12659/MSM.913780
更新日期:2019-05-30 00:00:00
abstract::BACKGROUND Cardiac resynchronization therapy (CRT) is efficacious in the treatment of chronic heart failure (CHF); however, because it is non-physiological, some patients are unresponsive. The present study used rate-adaptive atrioventricular delay (RAAVD) to track the physiological atrioventricular delay and investig...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.904348
更新日期:2017-08-17 00:00:00
abstract::BACKGROUND Spinal manipulation, particularly in cervical rotatory manipulation (CRM), has become increasingly popular in physical therapies, with satisfying effect. However, it is still unclear whether CRM affects internal carotid arteries (ICA) with mild carotid atherosclerosis (CAS), especially in hemodynamics. MATE...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.913351
更新日期:2019-03-31 00:00:00
abstract:BACKGROUND:We investigated the relationship between peripheral neuropathy and parameters of arterial stiffness and carotid intima media thickness (CIMT) in patients with type 2 diabetes mellitus (T2DM). MATERIAL AND METHODS:The study included 161 patients (80 females and 81 males), 69 of whom had peripheral neuropathy...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.892648
更新日期:2014-10-29 00:00:00
abstract:BACKGROUND:Inflammation and pulmonary injury caused by mechanical ventilation is a complex process in which long pentraxin 3 (PTX3) is suggested to play an important role. The effect of mechanical stretch on PTX3 mRNA and protein expression in human alveolar epithelial cells (A549) was investigated. MATERIAL/METHODS:I...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2009-05-01 00:00:00
abstract::BACKGROUND Age-related macular degeneration (AMD) is the leading cause of blindness in people aged 65 years and older in developed countries. The pathogenesis of AMD has been linked to mechanisms involving inflammation, oxidative stress, and basal laminar deposit formation between retinal pigment epithelium (RPE) cell...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.905311
更新日期:2018-01-10 00:00:00