Selective Targeting of Nav1.7 with Engineered Spider Venom-Based Peptides.

abstract：:Batrachotoxin (BTX) not only keeps the voltage-gated Na(+) channel open persistently but also reduces its single-channel conductance. Although a BTX receptor has been delimited within the inner cavity of Na(+) channels, how Na(+) ions flow through the BTX-bound permeation pathway remains unclear. In this report we tes...

journal_title：Channels (Austin, Tex.)

authors： Wang SY,Tikhonov DB,Mitchell J,Zhorov BS,Wang GK

更新日期：2007-05-01 00:00:00

abstract：:Andersen syndrome is an autosomal dominant disorder characterized by cardiac arrhythmias, periodic paralysis and dysmorphic features. Many Andersen syndrome cases have been associated with loss-of-function mutations in the inward rectifier K(+) channel Kir2.1 encoded by KCNJ2. Using engineered concatenated tetrameric ...

journal_title：Channels (Austin, Tex.)

authors： Ballester LY,Vanoye CG,George AL Jr

更新日期：2007-05-01 00:00:00

abstract：:Neonatal beta cells are functionally immature as they secrete less insulin than adults and lack of glucose response. The mechanisms that participate in the functional maturation of these cells are not known. Adult rat beta cells synthesize and secrete nerve growth factor (NGF) and express NGF receptors. NGF increases ...

journal_title：Channels (Austin, Tex.)

authors： Navarro-Tableros V,Fiordelisio T,Hernandez-Cruz A,Hiriart M

更新日期：2007-11-01 00:00:00

abstract：:We investigated effects of paramyotonia congenita mutations F1473S and F1705I on gating of skeletal muscle Na+ channels. We used on-cell recordings from Xenopus oocytes to compare fast inactivation and deactivation in wild-type and mutant channels. Then, we used gating current recordings to determine how these actions...

journal_title：Channels (Austin, Tex.)

authors： Groome JR,Larsen MF,Coonts A

更新日期：2008-01-01 00:00:00

abstract：:Inherited mutations of SCN5A, the gene that encodes Na(V)1.5, the alpha subunit of the principle voltage-gated Na(+) channel in the heart, cause congenital Long QT Syndrome variant 3 (LQT-3) by perturbation of channel inactivation. LQT-3 mutations induce small, but aberrant, inward current that prolongs the ventricula...

journal_title：Channels (Austin, Tex.)

authors： Bankston JR,Sampson KJ,Kateriya S,Glaaser IW,Malito DL,Chung WK,Kass RS

更新日期：2007-07-01 00:00:00

abstract：:N-type channels are located on dendrites and at pre-synaptic nerve terminals where they play a fundamental role in neurotransmitter release. They are potently regulated by the activation of a number of different types of pertussis toxin (PTX)-sensitive G alpha(i/o) coupled receptors, which results in voltage-dependent...

journal_title：Channels (Austin, Tex.)

authors： Kisilevsky AE,Zamponi GW

更新日期：2008-07-01 00:00:00

abstract：:Store-operated Ca2+ entry (SOCE) is a mechanism regulated by the filling state of the intracellular Ca2+ stores that requires the participation of the Ca2+ sensor STIM1, which communicates the Ca2+ content of the stores to the plasma membrane Ca2+-permeable channels. We have recently reported that Orai1 mediates the c...

journal_title：Channels (Austin, Tex.)

authors： Jardin I,Salido GM,Rosado JA

更新日期：2008-11-01 00:00:00

abstract：:Na(+)-coupled phosphate cotransporters of the SLC34 gene family catalyze the movement of inorganic phosphate (P(i)) across epithelia by using the free energy of the downhill electrochemical Na(+) gradient across the luminal membrane. Electrogenic (NaPi-IIa/b) and electroneutral (NaPi-IIc) isoforms prefer divalent P(i)...

journal_title：Channels (Austin, Tex.)

authors： Andrini O,Ghezzi C,Murer H,Forster IC

更新日期：2008-09-01 00:00:00

abstract：:Sodium proton exchangers (NHEs) constitute a large family of polytopic membrane protein transporters found in organisms across all domains of life. They are responsible for the exchange of protons for sodium ions. In archaea, bacteria, yeast and plants they provide increased salt tolerance by removing sodium in exchan...

journal_title：Channels (Austin, Tex.)

authors： Kemp G,Young H,Fliegel L

更新日期：2008-09-01 00:00:00

abstract：:KAAT1 and CAATCH1 are amino acid transporters cloned from the intestine of the lepidoptera Manduca sexta. They are members of the SLC6/NSS family, which groups membrane proteins that use Na(+), K(+), and Cl⁻ gradients for the coupled transport of amines and amino acids. The report of the atomic-resolution x-ray crysta...

journal_title：Channels (Austin, Tex.)

authors： Bettè S,Castagna M,Bossi E,Peres A,Sacchi VF

更新日期：2008-09-01 00:00:00

abstract：:Both structural and functional studies suggest that pH gating of the inward rectifier potassium (K) channel, Kir1.1 (ROMK), is mediated by the convergence of four hydrophobic leucines (one from each subunit) near the cytoplasmic bundle-crossing of the inner transmembrane helices. We tested this hypothesis by moving th...

journal_title：Channels (Austin, Tex.)

authors： Nanazashvili M,Li H,Palmer LG,Walters DE,Sackin H

更新日期：2007-01-01 00:00:00

abstract：:Deep cerebellar nuclear (DCN) neurons exhibit distinct phenotypes of rebound discharge following current-evoked membrane hyperpolarizations that arise from specific Ca(V)3 T-type Ca(2+) channel isoforms and Ca(2+)-activated K(+) channels. The probability of evoking rebound bursts following a brief train of GABAergic i...

journal_title：Channels (Austin, Tex.)

authors： Tadayonnejad R,Mehaffey WH,Anderson D,Turner RW

更新日期：2009-05-01 00:00:00

abstract：:Large-conductance Ca(2+)- and voltage-gated Slo1 BK channels are allosterically activated by depolarization and intracellular ligands such as Ca(2+). Of the two high-affinity Ca(2+) sensors present in the channel, the RCK1 sensor also mediates H(+)-dependent activation of the channel. In this study, we examined the co...

journal_title：Channels (Austin, Tex.)

authors： Hou S,Horrigan FT,Xu R,Heinemann SH,Hoshi T

更新日期：2009-07-01 00:00:00

abstract：:The K(V)7 (KCNQ) subfamily of voltage-gated K(+) channels consists of five members (K(V)7.1-K(V)7.5) giving rise to non-inactivating, and slowly activating/deactivating currents mainly expressed in cardiac (K(V)7.1) and neuronal (K(V)7.2-K(V)7.5) tissue. In the present study, using the cut-open oocyte voltage clamp, w...

journal_title：Channels (Austin, Tex.)

authors： Miceli F,Cilio MR,Taglialatela M,Bezanilla F

更新日期：2009-07-01 00:00:00

abstract：:K(ATP) channels play critical roles in many cellular functions by coupling cell metabolic status to electrical activity. First discovered in cardiomyocytes,(1) K(ATP) channels (comprised of Kir6.x and SUR subunits) have since been found in many other tissues, including pancreatic beta cells, skeletal muscle, smooth mu...

journal_title：Channels (Austin, Tex.)

authors： Kline CF,Hund TJ,Mohler PJ

更新日期：2010-01-01 00:00:00

abstract：:Existing molecular knowledge base of cardiovascular diseases is rudimentary because of lack of specific attribution to cell type and function. The aim of this study was to investigate cell-specific molecular remodeling in human atrial and ventricular myocytes associated with ischemic cardiomyopathy. Our strategy combi...

journal_title：Channels (Austin, Tex.)

authors： Gronich N,Kumar A,Zhang Y,Efimov IR,Soldatov NM

更新日期：2010-03-01 00:00:00

abstract：:Throughout the body, the epithelial Na(+) channel (ENaC) plays a critical role in salt and liquid homeostasis. In cystic fibrosis airways, for instance, improper regulation of ENaC results in hyperabsorption of sodium that causes dehydration of airway surface liquid. This dysregulation then contributes to mucus stasis...

journal_title：Channels (Austin, Tex.)

authors： Rollins BM,Garcia-Caballero A,Stutts MJ,Tarran R

更新日期：2010-07-01 00:00:00

abstract：:All eukaryotic and some prokaryotic ClC anion transport proteins have extensive cytoplasmic C-termini containing two cystathionine-β-synthase (CBS) domains. CBS domain secondary structure is highly conserved and consists of two α-helices and three β-strands arranged as β1-α1-β2-β3-α2. ClC CBS domain mutations cause mu...

journal_title：Channels (Austin, Tex.)

authors： Dave S,Sheehan JH,Meiler J,Strange K

更新日期：2010-07-01 00:00:00

abstract：:Low voltage-activated T-type calcium (Ca) channels contribute to the normal development of the heart and are also implicated in pathophysiological states such as cardiac hypertrophy. Functionally distinct T-type Ca channel isoforms can be generated by alternative splicing from each of three different T-type genes (Ca(...

journal_title：Channels (Austin, Tex.)

authors： David LS,Garcia E,Cain SM,Thau E,Tyson JR,Snutch TP

更新日期：2010-09-01 00:00:00

abstract：:Although highly selective Ca²(+) entry pathways play a critical role in agonist-activated Ca²(+) signals in non-excitable cells, only with the recent discovery of the Orai proteins have the first insights into the molecular nature of these pathways been possible. To date, just two such highly Ca²(+)-selective "Orai ch...

journal_title：Channels (Austin, Tex.)

authors： Thompson J,Mignen O,Shuttleworth TJ

更新日期：2010-09-01 00:00:00

abstract：:The Epithelial Na(+) Channel (ENaC) is an apical heteromeric channel that mediates Na(+) entry into epithelial cells from the luminal cell surface. ENaC is activated by proteases that interact with the channel during biosynthesis or at the extracellular surface. Meprins are cell surface and secreted metalloproteinases...

journal_title：Channels (Austin, Tex.)

authors： Garcia-Caballero A,Ishmael SS,Dang Y,Gillie D,Bond JS,Milgram SL,Stutts MJ

更新日期：2011-01-01 00:00:00

abstract：:Microglia have a swelling-activated Cl- current (which we call IClswell), and while some of its biophysical properties and functional roles have been elucidated, its molecular identity is unknown. To relate this current to cell functions and determine whether it is regulated by mechanisms other than cell swelling, it ...

journal_title：Channels (Austin, Tex.)

authors： Schlichter LC,Mertens T,Liu B

更新日期：2011-03-01 00:00:00

abstract：:Studies of the non-selective NaK channel as a model system provided detailed structural and functional insights into different factors that modulate selective ion conductance. The combination of structural and functional studies of WT- and mutant forms of NaK channel show that apparent conservation of the multiple bin...

journal_title：Channels (Austin, Tex.)

authors： Durdagi S,Noskov SY

更新日期：2011-05-01 00:00:00

abstract：:Despite significant progress in our understanding of the cellular and molecular mechanisms underlying sensory transduction and nociception, clinical pain management remains a considerable challenge in health care and basic research. The identification of the superfamily of transient receptor potential (TRP) cation cha...

journal_title：Channels (Austin, Tex.)

authors： Lapointe TK,Altier C

更新日期：2011-11-01 00:00:00

abstract：:Loss-of-function mutations in the pore-forming α subunit of the voltage-gated sodium channel 1.7 (Nav 1.7) cause congenital indifference to pain and anosmia. We used immunohistochemical techniques to study Nav 1.7 localization in the rat olfactory system in order to better understand its role in olfaction. We confirm ...

journal_title：Channels (Austin, Tex.)

authors： Rupasinghe DB,Knapp O,Blomster LV,Schmid AB,Adams DJ,King GF,Ruitenberg MJ

更新日期：2012-03-01 00:00:00

abstract：:The Drosophila light activated TRP and TRPL channels have been a model for TRPC channel gating. Several gating mechanisms have been proposed following experiments conducted on photoreceptor and tissue cultured cells. However, conclusive evidence for any mechanism is still lacking. Here, we show that the Drosophila TRP...

journal_title：Channels (Austin, Tex.)

authors： Lev S,Katz B,Minke B

更新日期：2012-03-01 00:00:00

abstract：:Bupivacaine is a local anesthetic compound belonging to the amino amide group. Its anesthetic effect is commonly related to its inhibitory effect on voltage-gated sodium channels. However, several studies have shown that this drug can also inhibit voltage-operated K(+) channels by a different blocking mechanism. This ...

journal_title：Channels (Austin, Tex.)

authors： Martín P,Enrique N,Palomo AR,Rebolledo A,Milesi V

更新日期：2012-05-01 00:00:00

abstract：:Activity of the bacterial mechanosensitive channels of small conductance MscS/MscK of E. coli was investigated under high hydrostatic pressure (HHP) using the "flying-patch" patch-clamp technique. The channels were gated by negative pipette voltage and their open probability was measured at HHP of 0.1 to 80 MPa. The c...

journal_title：Channels (Austin, Tex.)

authors： Petrov E,Rohde PR,Cornell B,Martinac B

更新日期：2012-07-01 00:00:00

abstract：:Scaffolding proteins are involved in the incorporation, anchoring, maintenance, and removal of AMPA receptors (AMPARs) at synapses, either through a direct interaction with AMPARs or via indirect association through auxiliary subunits of transmembrane AMPAR regulatory proteins (TARPs). Synaptic scaffolding molecule (S...

journal_title：Channels (Austin, Tex.)

authors： Danielson E,Metallo J,Lee SH

更新日期：2012-09-01 00:00:00

abstract：:TRPC4 and TRPC5 channels are important regulators of electrical excitability in both gastrointestinal myocytes and neurons. Much is known regarding the assembly and function of these channels including TRPC1 as a homotetramer or a heteromultimer and the roles that their interacting proteins play in controlling these e...

journal_title：Channels (Austin, Tex.)

authors： Kim H,Kim J,Jeon JP,Myeong J,Wie J,Hong C,Kim HJ,Jeon JH,So I

更新日期：2012-09-01 00:00:00