Abstract:
:The plasticizer di(2-ethylhexyl) phthalate (DEHP) and its hydrolysate mono(2-ethylhexyl) phthalate (MEHP) are major toxicants from plastics, but their association with hormone-dependent cancers has been controversial. We treated the human ovarian cancer cell lines SKOV3 and A2780 with low concentrations of DEHP/MEHP, and found that although no significant effect on cell proliferation was observed, ovarian cancer cell migration, invasion, and epithelial-mesenchymal transition (EMT) were promoted by submicromolar MEHP but not DEHP. Next, ovarian cancer patient data from The Cancer Genome Atlas (TCGA) were obtained and subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) supported enrichment and Kaplan-Meier survival analyses, which identified PI3K/Akt pathway as a pivotal signaling pathway in ovarian cancer. We found that 500 nM MEHP treatment significantly increased PIK3CA expression, which could be reversed by the knockdown of peroxisome proliferator-activated receptor alpha (PPARα). Silencing PIK3CA significantly suppressed the MEHP-induced migration, invasion and EMT. In addition, we validated that MEHP treatment promoted phosphorylation of Akt and degradation of IκB-α, thereby activating NF-κB and enhancing NF-κB nuclear translocation. In nude mice, MEHP exposure significantly promoted the metastasis of ovarian cancer xenografts, which could be suppressed by the treatment of PPARα inhibitor GW6471. Our findings showed that low-dose MEHP promoted ovarian cancer progression through activating PI3K/Akt/NF-κB pathway, in a PPARα-dependent manner.
journal_name
Sci Total Environjournal_title
The Science of the total environmentauthors
Leng J,Li H,Niu Y,Chen K,Yuan X,Chen H,Fu Z,Zhang L,Wang F,Chen C,Héroux P,Yang J,Zhu X,Lu W,Xia D,Wu Ydoi
10.1016/j.scitotenv.2021.147990keywords:
["Low-dose exposure","MEHP","Metastasis","Ovarian cancer","PI3K pathway","PPARα"]subject
Has Abstractpub_date
2021-10-10 00:00:00pages
147990eissn
0048-9697issn
1879-1026pii
S0048-9697(21)03061-8journal_volume
790pub_type
杂志文章相关文献
文献大全abstract::Morphological alterations of the cerebral vasculature as related to the permeability of plasma proteins and angiographic contrast media following unilateral cerebral angiography were studied. Both Evans blue albumin and horseradish peroxidase were employed as protein tracers for light and electron microscopy investiga...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)80017-5
更新日期:1999-01-12 00:00:00
abstract::Morphological alterations of the cerebral vasculature as related to the permeability of plasma proteins and angiographic contrast media following unilateral cerebral angiography were studied. Both Evans blue albumin and horseradish peroxidase were employed as protein tracers for light and electron microscopy investiga...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(98)00342-8
更新日期:1999-01-12 00:00:00
abstract:OBJECTIVE:To determine whether a sensitive and specific battery of tests (PD Battery) could identify a subset of asymptomatic first-degree relatives (FDRs) of PD patients who were significantly more impaired than age-matched normal control (NC) subjects. The PD Battery incorporates tests of motor function, olfaction, a...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(98)00371-4
更新日期:1999-02-02 00:00:00
abstract::Normally, ventricular APD exceeds the VERP. However, under specific circumstances this relation may change and can become inverse. This phenomenon of postrepolarization refractoriness may be caused by a decrease in excitability. The threshold current (TC) for pacing has never been quantified as a possible explanation ...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(98)00377-5
更新日期:1999-02-09 00:00:00
abstract::We performed single-breath washout (SBW) tests in which He and sulfur hexafluoride (SF6) were inspired throughout the vital capacity inspirations or were inhaled as discrete boluses at different points in the inspiration. Tests were performed in normal gravity (1 G) and in up to 27 s of microgravity (microG) during pa...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00019-4
更新日期:1999-03-09 00:00:00
abstract::The role of the otoliths in essential performances of human orientation is analyzed. The following interactions of the otoliths are considered: 1. The otoliths cooperate with graviceptors in the trunk in the perception of body posture. The truncal graviceptors turn out to yield on average 60% of the total gain. 2. The...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00044-3
更新日期:1999-04-05 00:00:00
abstract::Ictal SPECT is a highly sensitive method to localize the epileptogenic focus in refractory temporal lobe epilepsy in adults. In extratemporal epilepsy, sensitivity can be improved by subtracting interictal from ictal images and superimposing subtraction images on MRI. In children, such a procedure is potentially inter...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00091-1
更新日期:1999-07-01 00:00:00
abstract::A 64-channel electrode array was used to study the spatial and temporal characteristics of fast (>200 Hz) electrical oscillations recorded from the surface of rat cortex in both awake and anesthetized animals. Transient vibrissal displacements were effective in evoking oscillatory responses in the vibrissa/barrel fiel...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00099-6
更新日期:1999-08-01 00:00:00
abstract::We present data on the strain distribution and functional characteristics of the Ly-49 receptors A, C/I, D, and G2 on DX5+ natural killer (NK) cells. We have examined tyrosine phosphorylation of the Ly-49 molecules, regulation of NK cytotoxic functions, and in vivo marrow rejection capability. The flow cytometry resul...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00221-1
更新日期:1999-08-15 00:00:00
abstract:OBJECTIVE:To describe an unusual case of acute eosinophilic pneumonia (AEP) associated with hemodynamic instability. DESIGN:Case report, clinical. SETTINGS:Tertiary care intensive care unit (ICU). PATIENT:A single patient admitted to the ICU. INTERVENTIONS:Intravenous corticosteroids. MEASUREMENTS AND MAIN RESULTS...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00168-0
更新日期:1999-08-30 00:00:00
abstract::Small nucleolar RNAs (snoRNAs) constitute a group of more than 100 different stable RNA molecules that are found concentrated in the nucleolus where they are involved in the maturation of ribosomal RNA. Most snoRNAs are not produced from their own genes but are encoded in the introns of other genes, referred to as sno...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00200-4
更新日期:1999-09-01 00:00:00
abstract::Small nucleolar RNAs (snoRNAs) constitute a group of more than 100 different stable RNA molecules that are found concentrated in the nucleolus where they are involved in the maturation of ribosomal RNA. Most snoRNAs are not produced from their own genes but are encoded in the introns of other genes, referred to as sno...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00197-7
更新日期:1999-09-01 00:00:00
abstract::Small nucleolar RNAs (snoRNAs) constitute a group of more than 100 different stable RNA molecules that are found concentrated in the nucleolus where they are involved in the maturation of ribosomal RNA. Most snoRNAs are not produced from their own genes but are encoded in the introns of other genes, referred to as sno...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00241-7
更新日期:1999-09-01 00:00:00
abstract::Small nucleolar RNAs (snoRNAs) constitute a group of more than 100 different stable RNA molecules that are found concentrated in the nucleolus where they are involved in the maturation of ribosomal RNA. Most snoRNAs are not produced from their own genes but are encoded in the introns of other genes, referred to as sno...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00281-8
更新日期:1999-09-15 00:00:00
abstract::Small nucleolar RNAs (snoRNAs) constitute a group of more than 100 different stable RNA molecules that are found concentrated in the nucleolus where they are involved in the maturation of ribosomal RNA. Most snoRNAs are not produced from their own genes but are encoded in the introns of other genes, referred to as sno...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00264-8
更新日期:1999-09-15 00:00:00
abstract::Velar stops are phonetically characterized by two allophones-a palatal "g" when produced in front-vowel contexts and a velar "g" when produced in back-vowel contexts. Acoustic characterizations of velar stops based on plotting the onset and offset frequencies of the second formant transition clearly reveal two acousti...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00322-8
更新日期:1999-10-18 00:00:00
abstract::Dorsal carpal osteochondral injury is a major cause of reduced performance in horses undergoing high-intensity training. It was hypothesised that the mechanical behaviour and histology of cartilage are influenced by the intensity of exercise and by location within a joint. Relationships between histology and mechanica...
journal_title:The Science of the total environment
pub_type: 杂志文章,评审
doi:10.1016/s0048-9697(99)00122-9
更新日期:1999-09-30 00:00:00
abstract::Dorsal carpal osteochondral injury is a major cause of reduced performance in horses undergoing high-intensity training. It was hypothesised that the mechanical behaviour and histology of cartilage are influenced by the intensity of exercise and by location within a joint. Relationships between histology and mechanica...
journal_title:The Science of the total environment
pub_type: 杂志文章,多中心研究
doi:10.1016/s0048-9697(99)00132-1
更新日期:1999-09-30 00:00:00
abstract::Dorsal carpal osteochondral injury is a major cause of reduced performance in horses undergoing high-intensity training. It was hypothesised that the mechanical behaviour and histology of cartilage are influenced by the intensity of exercise and by location within a joint. Relationships between histology and mechanica...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00143-6
更新日期:1999-09-30 00:00:00
abstract::Dorsal carpal osteochondral injury is a major cause of reduced performance in horses undergoing high-intensity training. It was hypothesised that the mechanical behaviour and histology of cartilage are influenced by the intensity of exercise and by location within a joint. Relationships between histology and mechanica...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00155-2
更新日期:1999-09-30 00:00:00
abstract:BACKGROUND/AIM:Calcitonin gene-related peptide (CGRP) is a product of alternative RNA processing of the calcitonin gene. Its dual occurrence in nerves and endocrine cells makes it likely to be both a neuropeptide and an endocrine (paracrine) messenger. The purpose of this study was to determine whether CGRP-containing ...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00299-5
更新日期:1999-10-01 00:00:00
abstract::The influence of mutations in the 3' to 5' exonucleolytic proofreading epsilon-subunit of Escherichia coli DNA polymerase III on the genetic instabilities of the CGG.CCG and the CTG.CAG repeats that cause human hereditary neurological diseases was investigated. The dnaQ49(ts) and the mutD5 mutations destabilize the CG...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00394-0
更新日期:1999-12-06 00:00:00
abstract::The influence of mutations in the 3' to 5' exonucleolytic proofreading epsilon-subunit of Escherichia coli DNA polymerase III on the genetic instabilities of the CGG.CCG and the CTG.CAG repeats that cause human hereditary neurological diseases was investigated. The dnaQ49(ts) and the mutD5 mutations destabilize the CG...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00364-2
更新日期:1999-12-15 00:00:00
abstract::The influence of mutations in the 3' to 5' exonucleolytic proofreading epsilon-subunit of Escherichia coli DNA polymerase III on the genetic instabilities of the CGG.CCG and the CTG.CAG repeats that cause human hereditary neurological diseases was investigated. The dnaQ49(ts) and the mutD5 mutations destabilize the CG...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00417-9
更新日期:1999-12-15 00:00:00
abstract:PURPOSE:Our previous experience with the traditional management of infected prosthetic arterial grafts, which included graft excision and vein patch repair of the involved artery, was complicated by a high incidence of vein patch rupture. This study assessed the treatment of infected prosthetic grafts with subtotal gra...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00398-8
更新日期:1999-12-06 00:00:00
abstract::Learning and maintenance of memory in mice intraperitoneally (IP) injected with choline oxidase (ChO, 6 units/g), a hydrolytic enzyme for choline (Ch), were assessed by means of a step-through passive-avoidance task. The ChO treatment induced a hydrolysis of free Ch in plasma, which in turn, induced a decrease in cere...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00430-1
更新日期:2000-01-17 00:00:00
abstract::Learning and maintenance of memory in mice intraperitoneally (IP) injected with choline oxidase (ChO, 6 units/g), a hydrolytic enzyme for choline (Ch), were assessed by means of a step-through passive-avoidance task. The ChO treatment induced a hydrolysis of free Ch in plasma, which in turn, induced a decrease in cere...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00440-4
更新日期:2000-01-17 00:00:00
abstract::Learning and maintenance of memory in mice intraperitoneally (IP) injected with choline oxidase (ChO, 6 units/g), a hydrolytic enzyme for choline (Ch), were assessed by means of a step-through passive-avoidance task. The ChO treatment induced a hydrolysis of free Ch in plasma, which in turn, induced a decrease in cere...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00407-6
更新日期:2000-01-31 00:00:00
abstract:BACKGROUND:We present a retrospective analysis of a case series to evaluate closed intramedullary Kirschner wire (K-wire) fixation as a surgical technique in the treatment of femoral shaft fractures in children. METHODS:Fifty-three femoral shaft fractures (at various levels) were fixed by using closed intramedullary K...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00458-1
更新日期:2000-02-10 00:00:00
abstract::Escherichia coli R170, isolated from the urine of an infected patient, was resistant to expanded-spectrum cephalosporins, aztreonam, ciprofloxacin, and ofloxacin but was susceptible to amikacin, cefotetan, and imipenem. This particular strain contained three different plasmids that encoded two beta-lactamases with pIs...
journal_title:The Science of the total environment
pub_type: 杂志文章
doi:10.1016/s0048-9697(99)00469-6
更新日期:2000-02-28 00:00:00