Abstract：
:A fluorometry assay for trypsin sensitive determination has been presented. The fluorescence of the system at 370/445 nm is derived from thiochrome obtained by in-situ oxidation of thiamine. Based on the inner filter effect, cytochrome C (Cyt C) can quench the fluorescence at 445 nm effectively. Cyt C is specifically hydrolyzed by trypsin through an enzymatic reaction, giving rise to the enhancement of the fluorescence intensity. The change value of fluorescence intensity is proportional to trypsin concentration, which is successfully used for trypsin quantitative detection. This method exhibits good repeatability and selectivity with a detection limit of 0.15 μg mL-1 and a quantification limit of 0.50 μg mL-1 for trypsin sensing. Moreover, it is applied to detect trypsin in practical serum and urine samples with accurate results. The proposed assay is not only a promising candidate for trypsin determination in practical application but also a potentially valuable tool in urine comprehensive analysis and disease diagnosis.
journal_name
Talantajournal_title
Talantaauthors
Yao Z,Liu Y,Diao Y,Hu G,Qian Y,Li Zdoi
10.1016/j.talanta.2021.122614keywords:
["Cobaltic prussian blue analogs","Cyt C","Fluorescence","Thiamine","Trypsin"]subject
Has Abstractpub_date
2021-11-01 00:00:00pages
122614eissn
0039-9140issn
1873-3573pii
S0039-9140(21)00535-Xjournal_volume
234pub_type
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