当前位置： SCI文献检索 > 期刊下所有文献 > Signal strength controls the rate of polarization within CTLs during killing.

Signal strength controls the rate of polarization within CTLs during killing.

Abstract：

:Cytotoxic T lymphocytes (CTLs) are key effector cells in the immune response against viruses and cancers, killing targets with high precision. Target cell recognition by CTL triggers rapid polarization of intracellular organelles toward the synapse formed with the target cell, delivering cytolytic granules to the immune synapse. Single amino acid changes within peptides binding MHC class I (pMHCs) are sufficient to modulate the degree of killing, but exactly how this impacts the choreography of centrosome polarization and granule delivery to the target cell remains poorly characterized. Here we use 4D imaging and find that the pathways orchestrating killing within CTL are conserved irrespective of the signal strength. However, the rate of initiation along these pathways varies with signal strength. We find that increased strength of signal leads to an increased proportion of CTLs with prolonged dwell times, initial Ca2+ fluxes, centrosome docking, and granule polarization. Hence, TCR signal strength modulates the rate but not organization of effector CTL responses.

journal_name

J Cell Biol

journal_title

The Journal of cell biology

authors

Frazer GL,Gawden-Bone CM,Dieckmann NMG,Asano Y,Griffiths GM

doi

10.1083/jcb.202104093

subject

Has Abstract

pub_date

2021-10-04 00:00:00

issue

10

eissn

0021-9525

issn

1540-8140

pii

212498

journal_volume

220

pub_type

杂志文章

相关文献

文献大全
  • Telomere length dynamics and chromosomal instability in cells derived from telomerase null mice.

    abstract：:The careers which the graduates of one class from the Faculty of Medicine in The University of Sydney ("Class of '62") had chosen for themselves by the tenth anniversary of their graduation have been compiled and compared with data from other parts of Australia. ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.144.4.589

    authors： Hande MP,Samper E,Lansdorp P,Blasco MA

    更新日期：1999-02-22 00:00:00

  • Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubules.

    abstract：:The careers which the graduates of one class from the Faculty of Medicine in The University of Sydney ("Class of '62") had chosen for themselves by the tenth anniversary of their graduation have been compiled and compared with data from other parts of Australia. ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.144.4.721

    authors： Oegema K,Wiese C,Martin OC,Milligan RA,Iwamatsu A,Mitchison TJ,Zheng Y

    更新日期：1999-02-22 00:00:00

  • Direct imaging of DNA in living cells reveals the dynamics of chromosome formation.

    abstract：:Staphylococcal enterotoxin A (SEA) was labeled by the chloramine-T method with 125I to a specific activity of 68 to 300 muCi per mug of SEA and with 131I to specific activity of 8 to 218 muCi per mug of SEA. SEA was partially damaged and aggregated during the labeling and storage. The damage seemed not to be greatly d...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.144.5.813

    authors： Manders EM,Kimura H,Cook PR

    更新日期：1999-03-08 00:00:00

  • Control of mitotic spindle position by the Saccharomyces cerevisiae formin Bni1p.

    abstract：:Staphylococcal enterotoxin A (SEA) was labeled by the chloramine-T method with 125I to a specific activity of 68 to 300 muCi per mug of SEA and with 131I to specific activity of 8 to 218 muCi per mug of SEA. SEA was partially damaged and aggregated during the labeling and storage. The damage seemed not to be greatly d...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.144.5.947

    authors： Lee L,Klee SK,Evangelista M,Boone C,Pellman D

    更新日期：1999-03-08 00:00:00

  • A Cdc24p-Far1p-Gbetagamma protein complex required for yeast orientation during mating.

    abstract：:The aim of this study was to evaluate 111In-labelled bleomycin complex (111In-BLMC) SPET in the differentiation of high- and low-grade gliomas. Nineteen glioma patients, 14 with high-grade and five with low-grade tumours, were studied 1, 4 and 24 h after the injection of 111In-BLMC. In the high-grade glioma group, the...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.144.6.1187

    authors： Nern A,Arkowitz RA

    更新日期：1999-03-22 00:00:00

  • PTPmu regulates N-cadherin-dependent neurite outgrowth.

    abstract：:The aim of this study was to evaluate 111In-labelled bleomycin complex (111In-BLMC) SPET in the differentiation of high- and low-grade gliomas. Nineteen glioma patients, 14 with high-grade and five with low-grade tumours, were studied 1, 4 and 24 h after the injection of 111In-BLMC. In the high-grade glioma group, the...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.144.6.1323

    authors： Burden-Gulley SM,Brady-Kalnay SM

    更新日期：1999-03-22 00:00:00

  • The receptor recycling pathway contains two distinct populations of early endosomes with different sorting functions.

    abstract：:We have recently assigned a locus for familial psoriasis (PS) susceptibility to the region containing the epidermal differentiation complex gene cluster on chromosome 1q21. Gene S10OA7 maps within this cluster and is reported to be markedly over-expressed in the skin lesions of psoriatic patients. In order to analyse ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.145.1.123

    authors： Sheff DR,Daro EA,Hull M,Mellman I

    更新日期：1999-04-05 00:00:00

  • Prefoldin-nascent chain complexes in the folding of cytoskeletal proteins.

    abstract：:The first case of Mucha-Habermann disease-like drug eruptions due to Tegafur is reported. A 59-year-old man noticed various skin lesions after he had taken 300 mg of Tegafur daily for about 200 days. The patient had papulonecrotic eruptions on his trunk and extremities. The histology from a papular lesion revealed epi...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.145.2.265

    authors： Hansen WJ,Cowan NJ,Welch WJ

    更新日期：1999-04-19 00:00:00

  • Visualization of chemokine binding sites on human brain microvessels.

    abstract：:The first case of Mucha-Habermann disease-like drug eruptions due to Tegafur is reported. A 59-year-old man noticed various skin lesions after he had taken 300 mg of Tegafur daily for about 200 days. The patient had papulonecrotic eruptions on his trunk and extremities. The histology from a papular lesion revealed epi...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.145.2.403

    authors： Andjelkovic AV,Spencer DD,Pachter JS

    更新日期：1999-04-19 00:00:00

  • Hyphal death during colony development in Streptomyces antibioticus: morphological evidence for the existence of a process of cell deletion in a multicellular prokaryote.

    abstract：:Kikuchi-Fujimoto lymphadenitis is a distinctive entity which is easily recognised in its classical histology. However, cases which are biopsied in the early stage of the disease, before entering the necrotic phase may be misdiagnosed as non-Hodgkin's lymphoma. Ten cases of Kikuchi-Fujimoto disease which mimicked non-H...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.145.3.515

    authors： Miguélez EM,Hardisson C,Manzanal MB

    更新日期：1999-05-03 00:00:00

  • A filamentous cytoskeleton in vertebrate smooth muscle fibers.

    abstract：:Operations in foodservice establishments that have contributed to foodborne disease have been identified during investigation of outbreaks. Examination of foodborne disease surveillance data discloses need for a new or modified approach to foodborne disease control. Recommended constituents of a new approach include (...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.68.3.539

    authors： Cooke P

    更新日期：1976-03-01 00:00:00

  • Purification from black widow spider venom of a protein factor causing the depletion of synaptic vesicles at neuromuscular junctions.

    abstract：:The authors report the case of a patient with pancreatic and parathyroid adenomatosis and discuss whether it belongs to Wermer's syndrome. After a study of the various possible endocrine involvements, the complexity of hormonal inter-relations is studied, the prognosis and classification of multiple endocrine tumours ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.68.3.462

    authors： Frontali N,Ceccarelli B,Gorio A,Mauro A,Siekevitz P,Tzeng MC,Hurlbut WP

    更新日期：1976-03-01 00:00:00

  • Domains of axin involved in protein-protein interactions, Wnt pathway inhibition, and intracellular localization.

    abstract：:Tyrosine phosphorylation of the high affinity immunoglobulin (Ig)E receptor (FcepsilonRI) by the Src family kinase Lyn is the first known biochemical step that occurs during activation of mast cells and basophils after cross-linking of FcepsilonRI by antigen. The hypothesis that specialized regions in the plasma membr...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.145.4.741

    authors： Fagotto F,Jho Eh,Zeng L,Kurth T,Joos T,Kaufmann C,Costantini F

    更新日期：1999-05-17 00:00:00

  • Critical role for cholesterol in Lyn-mediated tyrosine phosphorylation of FcepsilonRI and their association with detergent-resistant membranes.

    abstract：:Tyrosine phosphorylation of the high affinity immunoglobulin (Ig)E receptor (FcepsilonRI) by the Src family kinase Lyn is the first known biochemical step that occurs during activation of mast cells and basophils after cross-linking of FcepsilonRI by antigen. The hypothesis that specialized regions in the plasma membr...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.145.4.877

    authors： Sheets ED,Holowka D,Baird B

    更新日期：1999-05-17 00:00:00

  • NH2-Terminal targeting motifs direct dual specificity A-kinase-anchoring protein 1 (D-AKAP1) to either mitochondria or endoplasmic reticulum.

    abstract：BACKGROUND:The study aims to evaluate the efficacy of resorbable clips in polydioxanone in laparoscopic surgery. METHODS:The authors report their personal experience regarding the use of Absolok (ABL) resorbable clips in laparoscopic surgery. Out of a total of 745 laparoscopic operations performed from September 1992 ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.145.5.951

    authors： Huang LJ,Wang L,Ma Y,Durick K,Perkins G,Deerinck TJ,Ellisman MH,Taylor SS

    更新日期：1999-05-31 00:00:00

  • R-Ras signals through specific integrin alpha cytoplasmic domains to promote migration and invasion of breast epithelial cells.

    abstract：BACKGROUND:The study aims to evaluate the efficacy of resorbable clips in polydioxanone in laparoscopic surgery. METHODS:The authors report their personal experience regarding the use of Absolok (ABL) resorbable clips in laparoscopic surgery. Out of a total of 745 laparoscopic operations performed from September 1992 ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.145.5.1077

    authors： Keely PJ,Rusyn EV,Cox AD,Parise LV

    更新日期：1999-05-31 00:00:00

  • A role for ubiquitination in mitochondrial inheritance in Saccharomyces cerevisiae.

    abstract：:Surgical intervention (ventricular drainage or decompressive craniotomy) may be necessary in patients with cerebellar infarction if mass effect develops. However, patient selection and timing of surgery remain controversial, and there are few data on clinical signs in the early course that are predictive for outcome. ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.145.6.1199

    authors： Fisk HA,Yaffe MP

    更新日期：1999-06-14 00:00:00

  • Three v-SNAREs and two t-SNAREs, present in a pentameric cis-SNARE complex on isolated vacuoles, are essential for homotypic fusion.

    abstract：CONTEXT:Experiments show that dental caries rates are higher among lead-exposed animals, but this association has not been established in humans. OBJECTIVE:To examine the relationship between blood lead levels and dental caries. DESIGN:Cross-sectional survey conducted from 1988 to 1994 that included a dental examinat...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.145.7.1435

    authors： Ungermann C,von Mollard GF,Jensen ON,Margolis N,Stevens TH,Wickner W

    更新日期：1999-06-28 00:00:00

  • Comparative studies of intracellular transport of secretory proteins.

    abstract：:The collagen that forms the egg case of the dogfish Scyliorhinus canicula is stored in bulk in the female nidamental glands. Here the collagen molecules are thought to undergo a series of distinct pH-dependent liquid crystalline aggregation phase changes before assembling into the final arrangement encountered in the ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.79.3.694

    authors： Tartakoff A,Vassalli P,Détraz M

    更新日期：1978-12-01 00:00:00

  • Pex22p of Pichia pastoris, essential for peroxisomal matrix protein import, anchors the ubiquitin-conjugating enzyme, Pex4p, on the peroxisomal membrane.

    abstract：:Behavior therapy has been shown to improve the functioning of institutionalized clients, but front-line staff often have difficulty implementing behavior therapy techniques. In the case described in this report, staff with inadequate training in behavior therapy inconsistently used negative and positive reinforcement ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.146.1.99

    authors： Koller A,Snyder WB,Faber KN,Wenzel TJ,Rangell L,Keller GA,Subramani S

    更新日期：1999-07-12 00:00:00

  • Neuropilin-1 mediates collapsin-1/semaphorin III inhibition of endothelial cell motility: functional competition of collapsin-1 and vascular endothelial growth factor-165.

    abstract：:Behavior therapy has been shown to improve the functioning of institutionalized clients, but front-line staff often have difficulty implementing behavior therapy techniques. In the case described in this report, staff with inadequate training in behavior therapy inconsistently used negative and positive reinforcement ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.146.1.233

    authors： Miao HQ,Soker S,Feiner L,Alonso JL,Raper JA,Klagsbrun M

    更新日期：1999-07-12 00:00:00

  • Kinetic control of multiple forms of Ca(2+) spikes by inositol trisphosphate in pancreatic acinar cells.

    abstract：:The hypothesis treated was that discrepancy between expected and experienced physical sensations (what is felt, seen, heard, tasted, and smelled) during a threatening experience will result in distress. The subjects were 84 children, 6 to 11 years of age, male and female. The threatening experience was orthopedic cast...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.146.2.405

    authors： Ito K,Miyashita Y,Kasai H

    更新日期：1999-07-26 00:00:00

  • Association of chromosome territories with the nuclear matrix. Disruption of human chromosome territories correlates with the release of a subset of nuclear matrix proteins.

    abstract：OBJECTIVE:To examine the descriptive epidemiology of weapon injuries not directly attributable to combat during armed conflict. DESIGN:Prospective cohort study. SETTING:Nangarhar region of Afghanistan, which experienced effective peace, intense fighting, and then peace over six months in 1996. SUBJECTS:608 people ad...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.146.3.531

    authors： Ma H,Siegel AJ,Berezney R

    更新日期：1999-08-09 00:00:00

  • Myotactin, a novel hypodermal protein involved in muscle-cell adhesion in Caenorhabditis elegans.

    abstract：OBJECTIVE:To examine the descriptive epidemiology of weapon injuries not directly attributable to combat during armed conflict. DESIGN:Prospective cohort study. SETTING:Nangarhar region of Afghanistan, which experienced effective peace, intense fighting, and then peace over six months in 1996. SUBJECTS:608 people ad...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.146.3.659

    authors： Hresko MC,Schriefer LA,Shrimankar P,Waterston RH

    更新日期：1999-08-09 00:00:00

  • Sid2p, a spindle pole body kinase that regulates the onset of cytokinesis.

    abstract：:The rostral part of the notochord reveals many peculiarities compared with the trunk mesoderm. Furthermore, its role in head formation and inductive processes in the head is not as well understood as the interaction of the trunk notochord with the spinal cord and somites. To interpret experimental and molecular biolog...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.146.4.777

    authors： Sparks CA,Morphew M,McCollum D

    更新日期：1999-08-23 00:00:00

  • Parameters that specify the timing of cytokinesis.

    abstract：:A decrease in lymphocyte signal-transduction molecules, described in cancer patients and patients with chronic infectious diseases, has been proposed as a possible mechanism leading to an impaired immune response in cancer patients. Here we report the effects of combination immunotherapy on the levels of T cell recept...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.146.5.981

    authors： Shuster CB,Burgess DR

    更新日期：1999-09-06 00:00:00

  • Regulation of cell contraction and membrane ruffling by distinct signals in migratory cells.

    abstract：:A decrease in lymphocyte signal-transduction molecules, described in cancer patients and patients with chronic infectious diseases, has been proposed as a possible mechanism leading to an impaired immune response in cancer patients. Here we report the effects of combination immunotherapy on the levels of T cell recept...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.146.5.1107

    authors： Cheresh DA,Leng J,Klemke RL

    更新日期：1999-09-06 00:00:00

  • Drosophila APC2 is a cytoskeletally-associated protein that regulates wingless signaling in the embryonic epidermis.

    abstract：OBJECTIVE:To report a case of angioedema associated with losartan administration. CASE SUMMARY:A 45-year-old white man with a history of hypertension and gout was treated with losartan/hydrochlorothiazide, allopurinol, and colchicine. The patient experienced two episodes of angioedema within a 10-hour period. On both ...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.146.6.1303

    authors： McCartney BM,Dierick HA,Kirkpatrick C,Moline MM,Baas A,Peifer M,Bejsovec A

    更新日期：1999-09-20 00:00:00

  • Stromal processing peptidase binds transit peptides and initiates their ATP-dependent turnover in chloroplasts.

    abstract：:AIDS in Africa is killing more people than all of Africa's armed conflicts during this century--this is what delegates heard at the 11th International Conference on AIDS and STDs in Lusaka, Zambia, September 12-16, 1999. The epidemic has been contained in the rich Northern Hemisphere. But why the difference? Delega...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.147.1.33

    authors： Richter S,Lamppa GK

    更新日期：1999-10-04 00:00:00

  • Cell wall stress depolarizes cell growth via hyperactivation of RHO1.

    abstract：:AIDS in Africa is killing more people than all of Africa's armed conflicts during this century--this is what delegates heard at the 11th International Conference on AIDS and STDs in Lusaka, Zambia, September 12-16, 1999. The epidemic has been contained in the rich Northern Hemisphere. But why the difference? Delega...

    journal_title：The Journal of cell biology

    pub_type： 杂志文章

    doi：10.1083/jcb.147.1.163

    authors： Delley PA,Hall MN

    更新日期：1999-10-04 00:00:00