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Extracellular acidosis enhances Zika virus infection both in human cells and ex-vivo tissue cultures from female reproductive tract.

Abstract：

:Zika virus (ZIKV) is a flavivirus transmitted by mosquitoes of the genus Aedes, but unlike other flaviviruses, ZIKV can be sexually transmitted by vaginal intercourse. The healthy vaginal pH ranges from 4.0 to 6.0, reaching values of 6.0-7.0 after semen deposition. Here, we report that low extracellular pH values (range 6.2-6.6) dramatically increase ZIKV infection on cell lines of different origin including some derived from the female genital tract and monocyte-derived macrophages. Furthermore, low pH significantly increased ZIKV infection of human ectocervix and endocervix cultured ex-vivo. Enhancement of infection by low pH was also observed using different ZIKV strains and distinct methods to evaluate viral infection, i.e. plaque assays, RT-PCR, flow cytometry, and fluorescence microscopy. Analysis of the mechanisms involved revealed that the enhancement of ZIKV infection induced by low pH was associated with increased binding of the viral particles to the heparan sulphate expressed on the target cell surface. Acidosis represents a critical but generally overlooked feature of the female genital tract, with major implications for sexual transmission diseases. Our results suggest that low vaginal pH might promote male-to-female transmission of ZIKV infection.

journal_name

Emerg Microbes Infect

journal_title

Emerging microbes & infections

authors

Varese A,Dantas E,Paletta A,Fitzgerald W,Di Diego García F,Cabrerizo G,Erra Diaz F,Defelipe LA,Pallares H,Dodes Traian M,Gamarnik A,Geffner J,Remes Lenicov F,Margolis L,Ceballos A

doi

10.1080/22221751.2021.1932606

keywords:

["ZIKV","ex-vivo tissues","extracellular acidosis","heparan sulphate","sexual transmission"]

subject

Has Abstract

pub_date

2021-12-01 00:00:00

pages

1169-1179

issue

1

issn

2222-1751

journal_volume

10

pub_type

杂志文章

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