Abstract:
:This study aimed to evaluate the effect of miR-10b on growth hormone (GH) in pituitary cells of Yanbian yellow cattle. According to analysis of GH and somatostatin receptor 2 (SSTR2) mRNA and protein expression levels, we found that miR-10b targeted 3'UTR of SSTR2. Compared with the negative control (NC) group, GH mRNA transcription and protein expression in pituitary cells of Yanbian yellow cattle were significantly increased by adding miR-10b mimics (p < .01), while these were significantly decreased by adding miR-10b inhibitor (p < .05); compared with the NC group, SSTR2 mRNA transcription and protein expression were significantly inhibited by the addition of miR-10b mimics (p < .01), while these were significantly increased by the addition of miR-10b inhibitor compared with the iNC group (p < .05). This study suggested that miR-10b could regulate GH level by regulating SSTR2 gene expression in pituitary cells of Yanbian yellow cattle.
journal_name
Anim Sci Jjournal_title
Animal science journal = Nihon chikusan Gakkaihoauthors
Ji J,Jin T,Lou A,Zhang R,Chen Y,Xiang S,Cui C,Yu L,Guan Ldoi
10.1111/asj.13420keywords:
["GH","SSTR2","Yanbian yellow cattle","miR-10b","pituitary cells"]subject
Has Abstractpub_date
2020-01-01 00:00:00pages
e13420issue
1eissn
1344-3941issn
1740-0929journal_volume
91pub_type
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