The Role of Pseudomonas aeruginosa ExoY in an Acute Mouse Lung Infection Model.

Abstract:

:The effector protein Exotoxin Y (ExoY) produced by Pseudomonas aeruginosa is injected via the type III secretion system (T3SS) into host cells. ExoY acts as nucleotidyl cyclase promoting the intracellular accumulation of cyclic nucleotides. To what extent nucleotidyl cyclase activity contributes to the pathogenicity of ExoY and which mechanisms participate in the manifestation of lung infection is still unclear. Here, we used an acute airway infection model in mice to address the role of ExoY in lung infection. In infected lungs, a dose-dependent phenotype of infection with bacteria-expressing ExoY was mirrored by haemorrhage, formation of interstitial oedema in alveolar septa, and infiltration of the perivascular space with erythrocytes and neutrophilic granulocytes. Analyses of the infection process on the cellular and organismal level comparing infections with Pseudomonas aeruginosa mutants expressing either nucleotidyl cyclase-active or -inactive ExoY revealed differential cytokine secretion, increased prevalence of apoptosis, and a break of lung barrier integrity in mice infected with cyclase-active ExoY. Notably, of all measured cyclic nucleotides, only the increase of cyclic UMP in infected mouse lungs coincides temporally with the observed early pathologic changes. In summary, our results suggest that the nucleotidyl cyclase activity of ExoY can contribute to P. aeruginosa acute pathogenicity.

journal_name

Toxins (Basel)

journal_title

Toxins

authors

Kloth C,Schirmer B,Munder A,Stelzer T,Rothschuh J,Seifert R

doi

10.3390/toxins10050185

subject

Has Abstract

pub_date

2018-05-04 00:00:00

issue

5

issn

2072-6651

pii

toxins10050185

journal_volume

10

pub_type

杂志文章

相关文献

Toxins文献大全
  • Innovative Immunization Strategies for Antivenom Development.

    abstract::Snakes, scorpions, and spiders are venomous animals that pose a threat to human health, and severe envenomings from the bites or stings of these animals must be treated with antivenom. Current antivenoms are based on plasma-derived immunoglobulins or immunoglobulin fragments from hyper-immunized animals. Although thes...

    journal_title:Toxins

    pub_type: 杂志文章,评审

    doi:10.3390/toxins10110452

    authors: Bermúdez-Méndez E,Fuglsang-Madsen A,Føns S,Lomonte B,Gutiérrez JM,Laustsen AH

    更新日期:2018-11-02 00:00:00

  • Spectral EMG Changes in Cervical Dystonia Patients and the Influence of Botulinum Toxin Treatment.

    abstract::Botulinum toxin (BoNT) injections in the dystonic muscles is the preferred treatment for Cervical Dystonia (CD), but the proper identification of the dystonic muscles remains a challenge. Previous studies showed decreased 8-14 Hz autospectral power in the electromyography (EMG) of splenius muscles in CD patients. Cumu...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins9090256

    authors: Nijmeijer SWR,de Bruijn E,Verhagen R,Forbes PA,Kamphuis DJ,Happee R,Tijssen MAJ,Koelman JHTM

    更新日期:2017-08-23 00:00:00

  • Comparative investigation of the efficacy of three different adsorbents against OTA-induced toxicity in broiler chickens.

    abstract::The aim of our study was to determine the efficacy of three different adsorbents, inorganic (modified zeolite), organic (esterified glucomannans) and mixed (inorganic and organic components, with the addition of enzymes), in protecting broilers from the toxic effects of ochratoxin A in feed. Broilers were fed diets co...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins7041174

    authors: Nedeljković-Trailović J,Trailović S,Resanović R,Milićević D,Jovanovic M,Vasiljevic M

    更新日期:2015-04-03 00:00:00

  • Ethylenediaminetetraacetic Acid Disodium Salt Acts as an Antifungal Candidate Molecule against Fusarium graminearum by Inhibiting DON Biosynthesis and Chitin Synthase Activity.

    abstract::Fusarium fungi are the cause of an array of devastating diseases affecting yield losses and accumulating mycotoxins. Fungicides can be exploited against Fusarium and deoxynivalenol (DON) production. However, Fusarium resistance to common chemicals has become a therapeutic challenge worldwide, which indicates that new ...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins13010017

    authors: Song XS,Gu KX,Gao J,Wang JX,Ding SC,Zhou M

    更新日期:2020-12-27 00:00:00

  • The Southeast Alaska Tribal Ocean Research (SEATOR) Partnership: Addressing Data Gaps in Harmful Algal Bloom Monitoring and Shellfish Safety in Southeast Alaska.

    abstract::Many communities in Southeast Alaska harvest shellfish such as mussels and clams as an important part of a subsistence or traditional diet. Harmful algal blooms (HABs) of phytoplankton such as Alexandrium spp. produce toxins that can accumulate in shellfish tissues to concentrations that can pose a hazard for human he...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins12060407

    authors: Harley JR,Lanphier K,Kennedy EG,Leighfield TA,Bidlack A,Gribble MO,Whitehead C

    更新日期:2020-06-19 00:00:00

  • The Unexpected Tuners: Are LncRNAs Regulating Host Translation during Infections?

    abstract::Pathogenic bacteria produce powerful virulent factors, such as pore-forming toxins, that promote their survival and cause serious damage to the host. Host cells reply to membrane stresses and ionic imbalance by modifying gene expression at the epigenetic, transcriptional and translational level, to recover from the to...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins9110357

    authors: Knap P,Tebaldi T,Di Leva F,Biagioli M,Dalla Serra M,Viero G

    更新日期:2017-11-03 00:00:00

  • Malting of Fusarium Head Blight-Infected Rye (Secale cereale): Growth of Fusarium graminearum, Trichothecene Production, and the Impact on Malt Quality.

    abstract::This project was initiated with the goal of investigating the malt quality of winter rye cultivars and hybrids grown in the United States in 2014 and 2015, but high levels of deoxynivalenol (DON) were subsequently found in many of the malt samples. DON levels in 75% of the investigated rye samples (n = 117) were actua...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins10090369

    authors: Jin Z,Gillespie J,Barr J,Wiersma JJ,Sorrells ME,Zwinger S,Gross T,Cumming J,Bergstrom GC,Brueggeman R,Horsley RD,Schwarz PB

    更新日期:2018-09-11 00:00:00

  • Naja mossambica mossambica Cobra Cardiotoxin Targets Mitochondria to Disrupt Mitochondrial Membrane Structure and Function.

    abstract::Cobra venom cardiotoxins (CVCs) can translocate to mitochondria to promote apoptosis by eliciting mitochondrial dysfunction. However, the molecular mechanism(s) by which CVCs are selectively targeted to the mitochondrion to disrupt mitochondrial function remains to be elucidated. By studying cardiotoxin from Naja moss...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins11030152

    authors: Zhang B,Li F,Chen Z,Shrivastava IH,Gasanoff ES,Dagda RK

    更新日期:2019-03-08 00:00:00

  • Limited Microcystin, Anatoxin and Cylindrospermopsin Production by Cyanobacteria from Microbial Mats in Cold Deserts.

    abstract::Toxic metabolites are produced by many cyanobacterial species. There are limited data on toxigenic benthic, mat-forming cyanobacteria, and information on toxic cyanobacteria from Central Asia is even more scarce. In the present study, we examined cyanobacterial diversity and community structure, the presence of genes ...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins12040244

    authors: Khomutovska N,Sandzewicz M,Łach Ł,Suska-Malawska M,Chmielewska M,Mazur-Marzec H,Cegłowska M,Niyatbekov T,Wood SA,Puddick J,Kwiatowski J,Jasser I

    更新日期:2020-04-11 00:00:00

  • Dimethylformamide Inhibits Fungal Growth and Aflatoxin B1 Biosynthesis in Aspergillus flavus by Down-Regulating Glucose Metabolism and Amino Acid Biosynthesis.

    abstract::Aflatoxins (AFs) are secondary metabolites produced by plant fungal pathogens infecting crops with strong carcinogenic and mutagenic properties. Dimethylformamide (DMF) is an excellent solvent widely used in biology, medicine and other fields. However, the effect and mechanism of DMF as a common organic solvent agains...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins12110683

    authors: Pan L,Chang P,Jin J,Yang Q,Xing F

    更新日期:2020-10-29 00:00:00

  • Assessment of the Antimicrobial Activity and the Entomocidal Potential of Bacillus thuringiensis Isolates from Algeria.

    abstract::This work represents the first initiative to analyze the distribution of B. thuringiensis in Algeria and to evaluate the biological potential of the isolates. A total of 157 isolates were recovered, with at least one isolate in 94.4% of the samples. The highest Bt index was found in samples from rhizospheric soil (0.4...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins9040139

    authors: Djenane Z,Nateche F,Amziane M,Gomis-Cebolla J,El-Aichar F,Khorf H,Ferré J

    更新日期:2017-04-13 00:00:00

  • The Therapeutic Effects and Pathophysiology of Botulinum Toxin A on Voiding Dysfunction Due to Urethral Sphincter Dysfunction.

    abstract::Neurogenic and non-neurogenic urethral sphincter dysfunction are common causes of voiding dysfunction. Injections of botulinum toxin A (BoNT-A) into the urethral sphincter have been used to treat urethral sphincter dysfunction (USD) refractory to conventional treatment. Since its first use for patients with detrusor s...

    journal_title:Toxins

    pub_type: 杂志文章,评审

    doi:10.3390/toxins11120728

    authors: Kao YL,Huang KH,Kuo HC,Ou YC

    更新日期:2019-12-13 00:00:00

  • Comparison of Anorectic Potencies of Type A Trichothecenes T-2 Toxin, HT-2 Toxin, Diacetoxyscirpenol, and Neosolaniol.

    abstract::Trichothecene mycotoxins are common contaminants in cereal grains and negatively impact human and animal health. Although anorexia is a common hallmark of type B trichothecenes-induced toxicity, less is known about the anorectic potencies of type A trichothecenes. The purpose of this study was to compare the anorectic...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins10050179

    authors: Zhang J,Zhang H,Liu S,Wu W,Zhang H

    更新日期:2018-04-29 00:00:00

  • Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast.

    abstract::Citrinin (CIT) and ochratoxin A (OTA) are important mycotoxins, which frequently co-contaminate foodstuff. In order to assess the toxicologic threat posed by the two mycotoxins separately or in combination, their biological effects were studied here using genomic transcription profiling and specific live cell gene exp...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins8100273

    authors: Vanacloig-Pedros E,Proft M,Pascual-Ahuir A

    更新日期:2016-09-22 00:00:00

  • Sea Anemones: Quiet Achievers in the Field of Peptide Toxins.

    abstract::Sea anemones have been understudied as a source of peptide and protein toxins, with relatively few examined as a source of new pharmacological tools or therapeutic leads. This is surprising given the success of some anemone peptides that have been tested, such as the potassium channel blocker from Stichodactyla helian...

    journal_title:Toxins

    pub_type: 杂志文章,评审

    doi:10.3390/toxins10010036

    authors: Prentis PJ,Pavasovic A,Norton RS

    更新日期:2018-01-08 00:00:00

  • The effects of a chactoid scorpion venom and its purified toxins on rat blood pressure and mast cells histamine release.

    abstract::The effect of the venom of the Chactoid family of scorpions on blood pressure was scantly investigated and was addressed in the present study using the venom of the Israeli scorpion, Scorpio maurus palmatus. Blood pressure in rats was monitored via cannulated femoral artery, while venom and toxins were introduced into...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins5081332

    authors: Ettinger K,Cohen G,Momic T,Lazarovici P

    更新日期:2013-07-29 00:00:00

  • Cytotoxic Properties of HT-2 Toxin in Human Chondrocytes: Could T3 Inhibit Toxicity of HT-2?

    abstract::Thyroid hormone triiodothyronine (T3) plays an important role in coordinated endochondral ossification and hypertrophic differentiation of the growth plate, while aberrant thyroid hormone function appears to be related to skeletal malformations, osteoarthritis, and Kashin-Beck disease. The T-2 toxin, present extensive...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins11110667

    authors: Zhang F,Lammi MJ,Shao W,Zhang P,Zhang Y,Wei H,Guo X

    更新日期:2019-11-15 00:00:00

  • Indole alkaloids from Fischerella inhibit vertebrate development in the zebrafish (Danio rerio) embryo model.

    abstract::Cyanobacteria are recognized producers of toxic or otherwise bioactive metabolite associated, in particular, with so-called "harmful algal blooms" (HABs) and eutrophication of freshwater systems. In the present study, two apparently teratogenic indole alkaloids from a freshwater strain of the widespread cyanobacterial...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins6123568

    authors: Walton K,Gantar M,Gibbs PD,Schmale MC,Berry JP

    更新日期:2014-12-22 00:00:00

  • CCD Based Detector for Detection of Abrin Toxin Activity.

    abstract::Abrin is a highly potent and naturally occurring toxin produced in the seeds of Abrus precatorius (Rosary Pea) and is of concern as a potential bioterrorism weapon. There are many rapid and specific assay methods to detect this toxic plant protein, but few are based on detection of toxin activity, critical to discern ...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins12020120

    authors: Rasooly R,Do P,Hernlem B

    更新日期:2020-02-14 00:00:00

  • Biomonitoring Data for Assessing Aflatoxins and Ochratoxin A Exposure by Italian Feedstuffs Workers.

    abstract::Mycotoxins exposure by inhalation and/or dermal contact is possible in different branches of industry especially where heavily dusty settings are present and the handling of dusty commodities is performed. This study aims to explore the validity of the biomonitoring as a tool to investigate the intake of mycotoxins in...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins11060351

    authors: De Santis B,Debegnach F,Sonego E,Mazzilli G,Buiarelli F,Ferri F,Giorgi Rossi P,Collini G,Brera C

    更新日期:2019-06-18 00:00:00

  • Individual and Combined Effects of Fumonisin B₁, Deoxynivalenol and Zearalenone on the Hepatic and Renal Membrane Lipid Integrity of Rats.

    abstract::(1) Background and (2) Methods: A 14-day in vivo, multitoxic (pure mycotoxins) rat experiment was conducted with zearalenone (ZEA; 15 μg/animal/day), deoxynivalenol (DON; 30 μg/animal/day) and fumonisin B₁ (FB₁; 150 μg/animal/day), as individual mycotoxins, binary (FD, FZ and DZ) and ternary combinations (FDZ), via ga...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins10010004

    authors: Szabó A,Szabó-Fodor J,Fébel H,Mézes M,Balogh K,Bázár G,Kocsó D,Ali O,Kovács M

    更新日期:2017-12-22 00:00:00

  • Role of Homologous Fc Fragment in the Potency and Efficacy of Anti-Botulinum Antibody Preparations.

    abstract::The only approved treatment for botulism relies on passive immunity which is mostly based on antibody preparations collected from hyper-immune horses. The IgG Fc fragment is commonly removed from these heterologous preparations to reduce the incidence of hyper-sensitivity reactions. New-generation therapies entering t...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins9060180

    authors: Torgeman A,Ozeri E,Ben David A,Diamant E,Rosen O,Schwartz A,Barnea A,Makovitzki A,Mimran A,Zichel R

    更新日期:2017-05-29 00:00:00

  • Assessment of Dried Blood Spots for Multi-Mycotoxin Biomarker Analysis in Pigs and Broiler Chickens.

    abstract::Dried blood spots (DBSs), a micro-sampling technique whereby a drop of blood is collected on filter paper has multiple advantages over conventional blood sampling regarding the sampling itself, as well as transportation and storage. This is the first paper describing the development and validation of a method for the ...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins11090541

    authors: Lauwers M,Croubels S,De Baere S,Sevastiyanova M,Romera Sierra EM,Letor B,Gougoulias C,Devreese M

    更新日期:2019-09-18 00:00:00

  • Variation of Fusarium Free, Masked, and Emerging Mycotoxin Metabolites in Maize from Agriculture Regions of South Africa.

    abstract::The presence of mycotoxins in cereal grain is a very important food safety issue with the occurrence of masked mycotoxins extensively investigated in recent years. This study investigated the variation of different Fusarium metabolites (including the related regulated, masked, and emerging mycotoxin) in maize from var...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins12030149

    authors: Ekwomadu TI,Dada TA,Nleya N,Gopane R,Sulyok M,Mwanza M

    更新日期:2020-02-28 00:00:00

  • Central Effects of Botulinum Neurotoxin-Evidence from Human Studies.

    abstract::For more than three decades, Botulinum neurotoxin (BoNT) has been used to treat a variety of clinical conditions such as spastic or dystonic disorders by inducing a temporary paralysis of the injected muscle as the desired clinical effect. BoNT is known to primarily act at the neuromuscular junction resulting in a bio...

    journal_title:Toxins

    pub_type: 杂志文章,评审

    doi:10.3390/toxins11010021

    authors: Weise D,Weise CM,Naumann M

    更新日期:2019-01-06 00:00:00

  • Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Hromatography-Tandem Mass Spectrometry Method.

    abstract::A liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3Ac-DON), 15-acetyldeoxynivalenol (15Ac-DON), DON-3-glucoside (DON-3Glc) nivalenol and fusarenone-X in feedstuffs. Different techniques of sample preparation were tested...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins12060362

    authors: Panasiuk Ł,Jedziniak P,Pietruszka K,Posyniak A

    更新日期:2020-06-01 00:00:00

  • Molecular Epidemiology of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus in Wild, Captive and Laboratory Rats: Effect of Habitat on the Nasal S. aureus Population.

    abstract::Rats are a reservoir of human- and livestock-associated methicillin-resistant Staphylococcus aureus (MRSA). However, the composition of the natural S. aureus population in wild and laboratory rats is largely unknown. Here, 144 nasal S. aureus isolates from free-living wild rats, captive wild rats and laboratory rats w...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins12020080

    authors: Raafat D,Mrochen DM,Al'Sholui F,Heuser E,Ryll R,Pritchett-Corning KR,Jacob J,Walther B,Matuschka FR,Richter D,Westerhüs U,Pikula J,van den Brandt J,Nicklas W,Monecke S,Strommenger B,van Alen S,Becker K,Ulrich RG,Hol

    更新日期:2020-01-24 00:00:00

  • Metabolism of the Marine Phycotoxin PTX-2 and Its Effects on Hepatic Xenobiotic Metabolism: Activation of Nuclear Receptors and Modulation of the Phase I Cytochrome P450.

    abstract::PTX-2 is a marine biotoxin frequently found in shellfish that can lead to food intoxication in humans. Information regarding PTX-2 metabolism is scarce, and little is known of its effect on xenobiotic-metabolizing enzymes (XME) or its molecular pathways. The aim of this study was consequently to examine PTX-2 Phase I ...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins9070212

    authors: Alarcan J,Dubreil E,Huguet A,Hurtaud-Pessel D,Hessel-Pras S,Lampen A,Fessard V,Le Hegarat L

    更新日期:2017-07-05 00:00:00

  • Immunogenicity of a Bivalent Non-Purified Recombinant Vaccine against Botulism in Cattle.

    abstract::Botulism is a potentially fatal intoxication caused by botulinum neurotoxins (BoNTs) produced mainly by Clostridium botulinum. Vaccination against BoNT serotypes C and D is the main procedure to control cattle botulism. Current vaccines contain formaldehyde-inactivated native BoNTs, which have a time-consuming product...

    journal_title:Toxins

    pub_type: 杂志文章

    doi:10.3390/toxins10100381

    authors: Moreira C Jr,Ferreira MRA,da Cunha CEP,Donassolo RA,Finger PF,Moreira GMSG,Otaka DY,de Sousa LA,Barbosa JD,Moreira ÂN,Salvarani FM,Conceição FR

    更新日期:2018-09-20 00:00:00

  • Mechanism of diphtheria toxin catalytic domain delivery to the eukaryotic cell cytosol and the cellular factors that directly participate in the process.

    abstract::Research on diphtheria and anthrax toxins over the past three decades has culminated in a detailed understanding of their structure function relationships (e.g., catalytic (C), transmembrane (T), and receptor binding (R) domains), as well as the identification of their eukaryotic cell surface receptor, an understandin...

    journal_title:Toxins

    pub_type: 杂志文章,评审

    doi:10.3390/toxins3030294

    authors: Murphy JR

    更新日期:2011-03-01 00:00:00