Abstract:
BACKGROUND:Invossa™ (TissueGene-C) is a cell and gene therapy for osteoarthritis. It is composed of primary human chondrocytes (hChonJ cells) and irradiated human chondrocytes modified to express TGF-β1 (hChonJb#7 cells). The hChonJ cells were isolated from a polydactyly donor, and TGF-β1 cDNA was delivered to the cells, generating hChonJb#7 cells. Since the cells are allogeneic, the concern of immune response against cells has been raised. In this study, we investigated the immunogenicity of allogenic human chondrocyte, hChonJ cells. METHODS:The immunological properties of hChonJ cells were investigated through the analysis of surface marker expression and the effect on allogeneic T cell proliferation. Flow cytometry and RT-PCR analysis were performed to analyze the surface marker expression related to immune response, such as major histocompatibility complex (MHC) class I, class II, T cell co-stimulatory molecules and T cell co-inhibitory molecules. A mixed lymphocyte reaction (MLR) was conducted to evaluate how allogeneic T cells would respond to hChonJ cells. RESULTS:We observed that hChonJ cells did not express MHC class II and T cell co-stimulatory molecules, but expressed T cell co-inhibitory molecule PD-L2. IFN-γ treatment induced the expression of PD-L1, and up-regulated the expression of PD-L2. Also, we observed that hChonJ cells did not stimulate T cell proliferation from a MHC-mismatched donor. Further, they could suppress the proliferation of activated T cells. We also observed that the blockade of PD-L1 and/or PD-L2 with specific neutralizing antibody could lead to the restoration of allo-reactive T cell proliferation. CONCLUSIONS:We showed that hChonJ cells were not immunogenic but immunosuppressive, and that this phenomenon was mediated by co-inhibitory molecules PD-L1 and PD-L2 on hChonJ cells in a contact-dependent manner.
journal_name
BMC Musculoskelet Disordjournal_title
BMC musculoskeletal disordersauthors
Lim CL,Lee YJ,Cho JH,Choi H,Lee B,Lee MC,Kim Sdoi
10.1186/s12891-017-1547-8subject
Has Abstractpub_date
2017-05-18 00:00:00pages
199issue
1issn
1471-2474pii
10.1186/s12891-017-1547-8journal_volume
18pub_type
杂志文章abstract:BACKGROUND:Neck-shoulder pain conditions, e.g., chronic trapezius myalgia, have been associated with sensory disturbances such as increased sensitivity to experimentally induced pain. This study investigated pain sensitivity in terms of bilateral pressure pain thresholds over the trapezius and tibialis anterior muscles...
journal_title:BMC musculoskeletal disorders
pub_type: 杂志文章
doi:10.1186/1471-2474-12-230
更新日期:2011-10-12 00:00:00
abstract:BACKGROUND:Although the pathogenesis of adolescent idiopathic scoliosis (AIS) remains controversial, genetic factors are thought to play key roles in the development of AIS. In a recent genome-wide association study, a polymorphism in the interleukin-17 receptor C (IL-17RC) gene was reported to be associated with the s...
journal_title:BMC musculoskeletal disorders
pub_type: 杂志文章
doi:10.1186/1471-2474-13-181
更新日期:2012-09-21 00:00:00
abstract:BACKGROUND:Fractures of the hand and wrist are one of the most common injuries seen in adults. The Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire has been developed as a patient-reported assessment of pain and disability to evaluate the outcome after hand and wrist injuries. Patient reported outcomes (...
journal_title:BMC musculoskeletal disorders
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更新日期:2018-06-30 00:00:00
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journal_title:BMC musculoskeletal disorders
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12891-016-1126-4
更新日期:2016-07-11 00:00:00
abstract:BACKGROUND:The purpose of this study was to test the hypothesis that a health and wellness coaching (HWC)-based intervention for fibromyalgia (FM) would result in sustained improvements in health and quality of life, and reductions in health care utilization. METHODS:Nine female subjects meeting American College of Rh...
journal_title:BMC musculoskeletal disorders
pub_type: 杂志文章
doi:10.1186/s12891-016-1316-0
更新日期:2016-11-08 00:00:00
abstract:BACKGROUND:Low back pain (LBP) is the most prevalent musculoskeletal condition and one the most common causes of disability in the developed nations. Anecdotally, there is a general assumption that LBP prevalence in Africa is comparatively lower than in developed countries. The aim of this review was to systematically ...
journal_title:BMC musculoskeletal disorders
pub_type: 杂志文章,评审
doi:10.1186/1471-2474-8-105
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pub_type: 杂志文章
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更新日期:2019-05-15 00:00:00
abstract:BACKGROUND:Cyclosprin A (CsA) has been widely used clinically to treat the patients who have undergone organ transplantation or acquired autoimmune disease. The purpose of this study is to determine the effects of three different doses of CsA (1.5, 7.5, 15 mg/kg body weight) on the skeletal biomechanical proprieties at...
journal_title:BMC musculoskeletal disorders
pub_type: 杂志文章
doi:10.1186/1471-2474-12-240
更新日期:2011-10-24 00:00:00
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pub_type: 杂志文章,meta分析
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更新日期:2010-05-10 00:00:00
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journal_title:BMC musculoskeletal disorders
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更新日期:2013-06-08 00:00:00
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journal_title:BMC musculoskeletal disorders
pub_type: 杂志文章,随机对照试验
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更新日期:2012-03-27 00:00:00
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更新日期:2018-08-07 00:00:00
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journal_title:BMC musculoskeletal disorders
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doi:10.1186/1471-2474-11-166
更新日期:2010-07-21 00:00:00
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doi:10.1186/1471-2474-3-22
更新日期:2002-10-03 00:00:00
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journal_title:BMC musculoskeletal disorders
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12891-017-1854-0
更新日期:2017-11-22 00:00:00
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doi:10.1186/1471-2474-10-41
更新日期:2009-04-23 00:00:00
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更新日期:2016-06-17 00:00:00
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更新日期:2020-05-30 00:00:00
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journal_title:BMC musculoskeletal disorders
pub_type: 杂志文章
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更新日期:2008-02-19 00:00:00
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更新日期:2016-01-18 00:00:00
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更新日期:2018-08-31 00:00:00
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pub_type: 杂志文章
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更新日期:2013-10-28 00:00:00
abstract:BACKGROUND:Screening for osteoporosis with dual-energy X-ray absorptiometry (DXA) is recommended for male HIV-infected patients only above the age of 50. Recently, trabecular bone score (TBS) has been introduced as a novel tool to assess bone microarchitecture using DXA of the lumbar spine. Few studies have reported TB...
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pub_type: 杂志文章
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更新日期:2020-02-10 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2013-10-24 00:00:00
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更新日期:2018-06-09 00:00:00