Abstract:
:Antisense oligonucleotides complementary to RNA targets promise generality and ease of drug design. The first systemically administered antisense drug was recently approved for treatment and others are in clinical development. Chemical modifications that increase the hybridization affinity of oligonucleotides are reasoned to confer higher potency, i.e., modified oligonucleotides can be dosed at lower concentrations to achieve the same effect. Surprisingly, shorter and less affine oligonucleotides sometimes display increased potency. To explain this apparent contradiction, increased uptake or decreased propensity to form structures have been suggested as possible mechanisms. Here, we provide an alternative explanation that invokes only the kinetics behind oligonucleotide-mediated cleavage of RNA targets. A model based on the law of mass action predicts, and experiments support, the existence of an optimal binding affinity. Exaggerated affinity, and not length per se, is detrimental to potency. This finding clarifies how to optimally apply high-affinity modifications in the discovery of potent antisense oligonucleotide drugs.
journal_name
Mol Ther Nucleic Acidsjournal_title
Molecular therapy. Nucleic acidsauthors
Pedersen L,Hagedorn PH,Lindholm MW,Lindow Mdoi
10.1038/mtna.2013.72subject
Has Abstractpub_date
2014-02-18 00:00:00pages
e149issn
2162-2531pii
mtna201372journal_volume
3pub_type
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
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journal_title:Molecular therapy. Nucleic acids
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/j.omtn.2019.11.038
更新日期:2020-06-05 00:00:00
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
doi:10.1016/j.omtn.2019.07.001
更新日期:2019-09-06 00:00:00
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
doi:10.1016/j.omtn.2018.01.004
更新日期:2018-06-01 00:00:00
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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更新日期:2019-09-06 00:00:00
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journal_title:Molecular therapy. Nucleic acids
pub_type: 已发布勘误
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
doi:10.1016/j.omtn.2019.05.008
更新日期:2019-09-06 00:00:00
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
doi:10.1038/mtna.2012.13
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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