Abstract：
:Mitochondria, the main source of energy of cells, play a significant role in aerobic respiration process. Some stimulants can result in changes of mitochondrial microenvironments such as viscosity, pH and polarity. Abnormal changes of mitochondrial viscosity have been shown to relate to pathological activities and diseases. Therefore, it is critical to focus our attention on mitochondrial viscosity under different conditions. A novel organic water-soluble molecule called JLQL that could monitor viscosity was conveniently synthesized in two steps. The near-infrared sensor with maximum emission wavelength of 734.6 nm and the Stokes shift of 134.6 nm consisted of a fluorophore and a mitochondrial-targeting moiety as an acceptor group; the two were connected by a double bond. The fluorescence intensity of the sensor increased 175 times with the enhancement of viscosity of a PBS-glycerol system. The interference of other microenvironments such as pH and polarity and other interference analytes could be reduced. JLQL could sensitively and selectively differentiate different levels of mitochondrial viscosity induced by monensin or nystatin. Furthermore, the probe may provide an attractive way to monitor real-time changes of viscosity during mitophagy. Possessing the above properties, JLQL can potentially be employed as a powerful tool for the observation of mitochondrial viscosity.
journal_name
Talantajournal_title
Talantaauthors
Zhang S,Zhang Y,Zhao L,Xu L,Han H,Huang Y,Fei Q,Sun Y,Ma P,Song Ddoi
10.1016/j.talanta.2021.122592keywords:
["Mitochondria targeting","Mitochondrial autophagy","Near infrared fluorescence","Viscosity"]subject
Has Abstractpub_date
2021-10-01 00:00:00pages
122592eissn
0039-9140issn
1873-3573pii
S0039-9140(21)00513-0journal_volume
233pub_type
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