Abstract：
:Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated nuclease (Cas) based biosensing system provides a novel genomic diagnostic tool for pathogenic detection. However, most of the discovered Cas effectors have poor single strand DNA (ssDNA) target recognition capability with the constraint of protospacer adjacent motif (PAM) sites, which are not suitable for universal pathogenic diagnosis. Herein, we developed a highly sensitive and specific fluorescence tool for bacterial detection by utilizing the unique collateral cleavage activity of a Cas14a1-mediated nucleic acid detection platform (CMP). We combine CMP with molecular amplification to build a CRISPR-Cas based bioanalysis technique, offering fast nucleic acid detection with high sensitivity and specificity. This technique can identify different species of pathogens in milk samples with excellent accuracy. The CMP technique is a promising platform for pathogenic genomic diagnostic in biomedicine and food safety field.
journal_name
Biosens Bioelectronjournal_title
Biosensors & bioelectronicsauthors
Ge X,Meng T,Tan X,Wei Y,Tao Z,Yang Z,Song F,Wang P,Wan Ydoi
10.1016/j.bios.2021.113350keywords:
["CRISPR-Cas","Cas14a1","Nucleic acid detection platform","Pathogenic detection"]subject
Has Abstractpub_date
2021-10-01 00:00:00pages
113350eissn
0956-5663issn
1873-4235pii
S0956-5663(21)00387-0journal_volume
189pub_type
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