Nuclear envelope mechanobiology: linking the nuclear structure and function.

abstract：:Fusing the inner and outer membranes of the nucleus, the nuclear pore complex (NPC) forms a selective portal which serves as the sole gateway of the nucleus. These aqueous translocation channels allow free diffusion of small molecules and ions, as well as receptor-mediated transport of large macromolecules. Over the l...

journal_title：Nucleus (Austin, Tex.)

authors： Maimon T,Medalia O

更新日期：2010-09-01 00:00:00

abstract：:The DNA damage response (DDR) is comprised of a network of proteins that respond to DNA damage. Mediator of DNA Damage Checkpoint 1 (MDC1) plays an early and important role in the DDR. Recent data show that MDC1 binds multiple proteins that participate in various aspects of the DDR, positioning it at the core of the D...

journal_title：Nucleus (Austin, Tex.)

authors： Coster G,Goldberg M

更新日期：2010-03-01 00:00:00

abstract：:The proper communication between organelles is essential for many aspects of eukaryotic life. The coordination of nuclear and cytoplasmic activities in particular is of pivotal importance and depends on transport in and out of the nucleus. The material which translocates through nuclear pores is diverse; it includes n...

journal_title：Nucleus (Austin, Tex.)

authors： Kodiha M,Crampton N,Shrivastava S,Umar R,Stochaj U

更新日期：2010-05-01 00:00:00

abstract：:The nuclear envelope (NE) is a barrier that separates nuclear from cytoplasmic processes. It is composed of an inner and outer nuclear membrane (INM, ONM), separated by the perinuclear space (PNS). The ONM is contiguous with the endoplasmic reticulum (ER), and thus, the lumen of the NE and that of the ER constitute on...

journal_title：Nucleus (Austin, Tex.)

authors： Herrmann H,Zwerger M

更新日期：2010-07-01 00:00:00

abstract：:The extensive and multifaceted traffic between nucleus and cytoplasm is handled by a single type of macromolecular assembly called the nuclear pore complex (NPC). While being readily accessible to ions and metabolites, the NPC imposes stringent selectivity on the passage of proteins and RNA, tightly regulating their t...

journal_title：Nucleus (Austin, Tex.)

authors： Kapon R,Naim B,Zbaida D,Nevo R,Tsabari O,Reich Z

更新日期：2010-11-01 00:00:00

abstract：:The evolutionary conserved protein Sem1/Dss1 is a bona fide subunit of the regulatory particle (RP) of the proteasome and in mammalian cells stabilizes the tumor suppressor protein BRCA2. A recent study from our laboratory has revealed an unexpected non- proteasomal role of Sem1 in mRNA export. We found that Sem1, ind...

journal_title：Nucleus (Austin, Tex.)

authors： Faza MB,Kemmler S,Panse VG

更新日期：2010-01-01 00:00:00

abstract：:The heterogenous nuclear ribonucleoprotein G (hnRNP G) controls the alternative splicing of several pre-mRNas. While hnRNP G displays an amino terminal RNA recognition motif (RRM), we find that this motif is paradoxically not implicated in the recruitment of hnRNP G to nascent transcripts in amphibian oocytes. In fact...

journal_title：Nucleus (Austin, Tex.)

authors： Kanhoush R,Beenders B,Perrin C,Moreau J,Bellini M,Penrad-Mobayed M

更新日期：2010-01-01 00:00:00

abstract：:Interphase nuclear architecture is disrupted and rapidly reformed with each cell division cycle. Successive cell generations exhibit a "memory" of this nuclear architecture, as well as for gene expression. Furthermore, many features of nuclear and mitotic chromosome structure are recognizably species and tissue specif...

journal_title：Nucleus (Austin, Tex.)

authors： Olins AL,Langhans M,Monestier M,Schlotterer A,Robinson DG,Viotti C,Zentgraf H,Zwerger M,Olins DE

更新日期：2011-01-01 00:00:00

abstract：:Trafficking between the nucleoplasm and the cytoplasm occurs through the nuclear pore complex (NPC), which consists of large multiprotein complexes. Over the last several years, major progress has been made in both structural determination of the entire assembly of the NPC in yeast and animal cells. By contrast, the p...

journal_title：Nucleus (Austin, Tex.)

authors： Tamura K,Hara-Nishimura I

更新日期：2011-05-01 00:00:00

abstract：:Proteasomes localize to different compartments in eukaryotic cells, but the details of this dynamic distribution are poorly understood. Here, we discuss evidence linking proteasome activity to specific intranuclear locations and the potential mechanisms that may target these large proteolytic machines to the nucleus. ...

journal_title：Nucleus (Austin, Tex.)

authors： Savulescu AF,Rotem A,Harel A

更新日期：2011-07-01 00:00:00

abstract：:Defined as a chromatin structure that remains condensed throughout the cell cycle heterochromatin is generally transcriptionally silent and is characterized by a specific molecular signature. Constitutive heterochromatin at the pericentromere is a conserved feature throughout evolution, which impacts genome stability....

journal_title：Nucleus (Austin, Tex.)

authors： Almouzni G,Probst AV

更新日期：2011-09-01 00:00:00

abstract：:The active and inactive X (Xa;Xi) territory with its seemingly highly compacted Barr body in nuclei of female mammalian cells provide a key example for studies of structure/function relationships in homologous chromosomes with different functional properties. Here we used about 300 human X-specific large insert clones...

journal_title：Nucleus (Austin, Tex.)

authors： Teller K,Illner D,Thamm S,Casas-Delucchi CS,Versteeg R,Indemans M,Cremer T,Cremer M

更新日期：2011-09-01 00:00:00

abstract：:Changes in the nuclear structure and function during the cell cycle are thought to be correlated with lamins phosphorylation. Here, we report the identification of new in vivo phosphorylation sites on Drosophila melanogater lamin Dm using immunoisolation and mass spectrometry with collision-induced peptide fragmentati...

journal_title：Nucleus (Austin, Tex.)

authors： Zaremba-Czogalla M,Gagat P,Kozioł K,Dubińska-Magiera M,Sikora J,Dadlez M,Rzepecki R

更新日期：2011-09-01 00:00:00

abstract：:Hutchinson-Gilford Progeria Syndrome (HGPS) is a severe premature aging syndrome that affects children. These children display characteristics associated with normal aging and die young usually from cardiovascular problems or stroke. Classical HGPS is caused by mutations in the gene encoding the nuclear structural pro...

journal_title：Nucleus (Austin, Tex.)

authors： Bridger JM,Eskiw CH,Makarov EM,Tree D,Kill IR

更新日期：2011-11-01 00:00:00

abstract：:Computational image analysis is used in many areas of biological and medical research, but advanced techniques including machine learning remain underutilized. Here, we used automated segmentation and shape analyses, with pre-defined features and with computer generated components, to compare nuclei from various prema...

journal_title：Nucleus (Austin, Tex.)

authors： Choi S,Wang W,Ribeiro AJ,Kalinowski A,Gregg SQ,Opresko PL,Niedernhofer LJ,Rohde GK,Dahl KN

更新日期：2011-11-01 00:00:00

abstract：:Understanding the basis for multipotency, whereby stem cells and other progenitors can differentiate into certain tissues and not others, provides insights into the mechanism of cell programming in development, homeostasis, and disease. We recently reported a screen of diverse chromatin marks to obtain clues about chr...

journal_title：Nucleus (Austin, Tex.)

authors： Xu CR,Zaret KS

更新日期：2012-03-01 00:00:00

abstract：:The defining feature of eukaryotic cells is the double lipid bilayer of the nuclear envelope (NE) that serves as a physical barrier separating the genome from the cytosol. Nuclear pore complexes (NPCs) are embedded in the NE to facilitate transport of proteins and other macromolecules into and out of the nucleus. In f...

journal_title：Nucleus (Austin, Tex.)

authors： Jaspersen SL,Ghosh S

更新日期：2012-05-01 00:00:00

abstract：:In recent years there has been considerable and growing interest in the 3-dimensional organization of genomes. In this manuscript we present an integrated computational-molecular study that produces an ensemble of high-resolution 3-dimensional conformations of the budding yeast genome. The compaction, folding and spat...

journal_title：Nucleus (Austin, Tex.)

authors： Gehlen LR,Gruenert G,Jones MB,Rodley CD,Langowski J,O'Sullivan JM

更新日期：2012-07-01 00:00:00

abstract：:We recently identified a set of chromosome domains that are early replicating uniquely in pluripotent cells. Their switch from early to late replication occurs just prior to germ layer commitment, associated with a stable form of gene silencing that is difficult to reverse. Here, we discuss results demonstrating that ...

journal_title：Nucleus (Austin, Tex.)

authors： Takebayashi S,Ryba T,Gilbert DM

更新日期：2012-11-01 00:00:00

abstract：:Actin is a key player for nuclear structure and function regulating both chromosome organization and gene activity. In the cell nucleus actin interacts with many different proteins. Among these proteins several studies have identified classical nuclear factors involved in chromatin structure and function, transcriptio...

journal_title：Nucleus (Austin, Tex.)

authors： Percipalle P

更新日期：2013-01-01 00:00:00

abstract：:Numerous molecular details of intracellular mRNA processing have been revealed in recent years. However, the export process of single native mRNA molecules, the actual translocation through the nuclear pore complex (NPC), could not yet be examined in vivo. The problem is observing mRNA molecules without interfering wi...

journal_title：Nucleus (Austin, Tex.)

authors： Kaminski T,Siebrasse JP,Kubitscheck U

更新日期：2013-01-01 00:00:00

abstract：:Mitosis is the process of one cell dividing into two daughters, such that each inherits a single and complete copy of the genome of their mother. This is achieved through the equal segregation of the sister chromatids between the daughter cells. However, beyond this simple principle, the partitioning of other cellular...

journal_title：Nucleus (Austin, Tex.)

authors： Boettcher B,Barral Y

更新日期：2013-05-01 00:00:00

abstract：:A major challenge of modern human biology is to understand how a differentiated somatic cell integrates the response to external signals in the complex context of basic metabolic and tissue-specific gene expression programs. This requires exploring two interconnected basic processes: the signaling network and the glob...

journal_title：Nucleus (Austin, Tex.)

authors： Ballaré C,Zaurin R,Vicent GP,Beato M

更新日期：2013-05-01 00:00:00

abstract：:Emerin, a conserved LEM-domain protein, is among the few nuclear membrane proteins for which extensive basic knowledge--biochemistry, partners, functions, localizations, posttranslational regulation, roles in development and links to human disease--is available. This review summarizes emerin and its emerging roles in ...

journal_title：Nucleus (Austin, Tex.)

authors： Berk JM,Tifft KE,Wilson KL

更新日期：2013-07-01 00:00:00

abstract：:In the cytoplasm, actin filaments form crosslinked networks that enable eukaryotic cells to transport cargo, change shape, and move. Actin is also present in the nucleus but, in this compartment, its functions are more cryptic and controversial. If we distill the substantial literature on nuclear actin down to its ess...

journal_title：Nucleus (Austin, Tex.)

authors： Belin BJ,Mullins RD

更新日期：2013-07-01 00:00:00

abstract：:Sun proteins and Nesprins are two families of proteins whose direct interactions across the nuclear envelope provide for the core of Linkers of the Nucleoskeleton to the Cytoskeleton (LINC complexes) that physically connect the nucleus interior to cytoskeletal networks. Whereas LINC complexes play essential roles in n...

journal_title：Nucleus (Austin, Tex.)

authors： Razafsky DS,Ward CL,Kolb T,Hodzic D

更新日期：2013-09-01 00:00:00

abstract：:Nucleosomes confer a barrier to processes that require access to the eukaryotic genome such as transcription, DNA replication and repair. A variety of ATP-dependent nucleosome remodeling machines and ATP-independent histone chaperones facilitate nucleosome dynamics by depositing or evicting histones and unwrapping the...

journal_title：Nucleus (Austin, Tex.)

authors： Hondele M,Ladurner AG

更新日期：2013-11-01 00:00:00

abstract：:22q11.2 deletion syndrome (22q11DS) results from a hemizygous microdeletion on chromosome 22 and is characterized by extensive phenotypic variability. Penetrance of signs, including congenital heart, craniofacial, and neurobehavioral abnormalities, varies widely and is not well correlated with genotype. The three-dime...

journal_title：Nucleus (Austin, Tex.)

authors： Zeitz MJ,Lerner PP,Ay F,Van Nostrand E,Heidmann JD,Noble WS,Hoffman AR

更新日期：2013-11-01 00:00:00

abstract：:Caloric restriction (CR) is generally linked to lifespan extension in various organisms and may limit age-associated diseases. Processes through which caloric restriction promotes lifespan include obesity-countering weight loss, increased DNA repair, control of ribosomal and telomeric DNA repeats, mitochondrial regula...

journal_title：Nucleus (Austin, Tex.)

authors： Szafranski K,Mekhail K

更新日期：2014-01-01 00:00:00

abstract：:Alus are transposable elements belonging to the short interspersed element family. They occupy over 10% of human genome and have been spreading through genomes over the past 65 million years. In the past, they were considered junk DNA with little function that took up genome volumes. Today, Alus and other transposable...

journal_title：Nucleus (Austin, Tex.)

authors： Wang C,Huang S

更新日期：2014-03-01 00:00:00