Enhancing the take of injected adipose tissue by a simple method for concentrating fat cells.

Abstract:

:Injection of aspirated fat for the correction of tissue defects is a common procedure in plastic surgery. The reported rates of fat cell survival vary greatly in the medical literature, and different techniques of harvesting, processing, and reinjecting the fat cells are claimed to be responsible for these differences. However, there is no agreement concerning the best way to process the harvested fat before reinjection. The present study was initiated to examine and evaluate the effect of a simple method of isolating the fat particles on the outcome of fat graft survival. In this study, the nude mouse model was used to examine the survival and take of the fat graft concentrated before injection by the cumbersome recommended closed centrifugation technique in comparison with the authors' recommended open method, using an operating room cotton towel as a platform for concentrating the fat cells and separating them from fluids, oil, and debris. One milliliter of concentrated human fat cells preprocessed by towel separation was injected into the nuchal subcutis of 11 nude mice in the study group, and the same amount of fat that was preprocessed by centrifugation was injected into 11 control mice. Injected fat survived in both groups. No significant differences were found regarding fat graft weight and volume, although a tendency for better survival was noticed in the experimental group. Histologic evaluation of the grafts revealed significantly less fibrosis within the study group, meaning that the quality of the fat grafts was better. The authors found this method to be simple, cheap, and friendly to the surgeon in comparison with traditional processing using the centrifuge.

journal_name

Plast Reconstr Surg

authors

Ramon Y,Shoshani O,Peled IJ,Gilhar A,Carmi N,Fodor L,Risin Y,Ullmann Y

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

197-201; discsussion 202-3

issue

1

eissn

0032-1052

issn

1529-4242

pii

00006534-200501000-00026

journal_volume

115

pub_type

杂志文章
  • Effect of Cryopreservation on Human Adipose Tissue and Isolated Stromal Vascular Fraction Cells: In Vitro and In Vivo Analyses.

    abstract:BACKGROUND:Adipose tissue is a source of adipose-derived stromal/stem cells for tissue engineering and reconstruction and a tissue source for fat grafts. Although liposuction is a simple procedure for the harvest of adipose tissue, the repetition of this surgical intervention can cause adverse effects to the patient an...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000004030

    authors: Zanata F,Bowles A,Frazier T,Curley JL,Bunnell BA,Wu X,Wade J,Devireddy R,Gimble JM,Ferreira LM

    更新日期:2018-02-01 00:00:00

  • Measuring outcomes in aesthetic surgery: a comprehensive review of the literature.

    abstract::Outcomes research examines the end results of medical interventions, taking into account patients' experiences, preferences, and values. The purpose of assessing outcomes is to provide evidence on which to base clinical decisions. The assessment of outcomes in aesthetic surgery is especially pertinent because patient ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,meta分析

    doi:10.1097/01.PRS.0000036041.67101.48

    authors: Ching S,Thoma A,McCabe RE,Antony MM

    更新日期:2003-01-01 00:00:00

  • Mammaplasty-associated mycobacterial infection: a survey of plastic surgeons.

    abstract::In a 3 1/2-year period, periprosthetic infections due to Mycobacterium fortuitum complex organisms complicated augmentation mammaplasty in at least 17 women. To determine the magnitude of the problem and to identify possible risk factors for infection, we conducted a questionnaire survey of 2062 members of the America...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198308000-00007

    authors: Clegg HW,Bertagnoll P,Hightower AW,Baine WB

    更新日期:1983-08-01 00:00:00

  • Sagittal craniosynostosis outcome assessment for two methods and timings of intervention.

    abstract::A retrospective quantitative analysis of 40 infants who underwent surgery for sagittal craniosynostosis was conducted to determine whether any difference in outcome, with respect to cranial index (cranial width/cranial length x 100), could be associated with either the age at surgery or the extent of the operation. Ch...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199905060-00004

    authors: Panchal J,Marsh JL,Park TS,Kaufman B,Pilgram T,Huang SH

    更新日期:1999-05-01 00:00:00

  • Bilateral cauliflower ear deformity: an unusual presentation of cutaneous Rosai-Dorfman disease.

    abstract::This case illustrates the variety of clinical presentations of Rosai-Dorfman disease. In this case, the disease presented as bilateral cauliflower ear deformity and was diagnosed on the basis of typical pathological findings. Although the cause of this disease is not fully understood, it is hoped that, with increased ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000105646.49729.3f

    authors: Oo KK,Pang YT,Thamboo TP

    更新日期:2004-03-01 00:00:00

  • Effect of Body Mass Index on Outcomes after Prepectoral Breast Reconstruction.

    abstract:BACKGROUND:Body mass index has been shown to be a predictor of outcomes after subpectoral expander/implant reconstruction, with every unit increase in body mass index increasing the risk of complications by approximately 6 percent. The effect of body mass index on complications after prepectoral reconstruction has not ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000005901

    authors: Gabriel A,Sigalove S,Sigalove NM,Storm-Dickerson TL,Pope N,Rice J,Maxwell GP

    更新日期:2019-09-01 00:00:00

  • Jejunal free autograft: analysis of complications and their resolution.

    abstract::Review of 101 patients who underwent 111 free jejunal autografts has demonstrated an absolute procedural failure rate of 13.5 percent. Salvage reconstruction with a second jejunum was successful in six of nine patients and one third-time jejunum was successful, giving an overall salvage rate of 70 percent. There were ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:

    authors: Coleman JJ 3rd,Tan KC,Searles JM,Hester TR,Nahai F

    更新日期:1989-10-01 00:00:00

  • An operating table for hand surgery.

    abstract::The construction details for an inexpensive, easy-to-build operating table for hand surgery have been described. The table was specifically designed according to the needs of those working with it. It has been in daily use for over 2 years and has proved to be a popular product with all staff members. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198802000-00025

    authors: Bowen V,Chang P

    更新日期:1988-02-01 00:00:00

  • Current surgical management of bilateral cleft lip in North America.

    abstract:BACKGROUND:This study was undertaken to determine contemporary surgical management of bilateral cleft lip. METHODS:A survey was sent to North American cleft surgeons. Variables included number of bilateral cleft lip repairs per year, surgical specialty, and methods of nasolabial correction. The authors analyzed possib...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,多中心研究

    doi:10.1097/PRS.0b013e31824ecbd3

    authors: Tan SPK,Greene AK,Mulliken JB

    更新日期:2012-06-01 00:00:00

  • Magnetic resonance imaging characterization of orbital changes with age and associated contributions to lower eyelid prominence.

    abstract:BACKGROUND:Multiple orbital aging models have been suggested to explain the progressive development of lower eyelid prominence. Objective data to support these theories are limited, however. METHODS:Orbital anatomy was measured with high-resolution orbital magnetic resonance imaging in the quasi-sagittal plane paralle...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0b013e3181811ce8

    authors: Darcy SJ,Miller TA,Goldberg RA,Villablanca JP,Demer JL,Rudkin GH

    更新日期:2008-09-01 00:00:00

  • Evolution of the lateral canthoplasty: techniques and indications.

    abstract::Lateral canthoplasty is a useful method to restore eyelid function and to protect the ocular surfaces. The success of the procedure depends on the proper analysis of periorbital anatomy as it relates to the specific indication for lateral canthoplasty. We report the experience with 1565 lateral canthoplasties with emp...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199711000-00003

    authors: Glat PM,Jelks GW,Jelks EB,Wood M,Gadangi P,Longaker MT

    更新日期:1997-11-01 00:00:00

  • Transmucosal Pterygomaxillary Separation in the Le Fort I Osteotomy.

    abstract::The Le Fort I osteotomy is a versatile operation for correction of developmental, congenital, and posttraumatic deformities of the lower midface. One of the challenges of the osteotomy is pterygomaxillary separation, with the potential for unfavorable fractures to the orbit/skull base or vascular injury. A modified te...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000006751

    authors: Susarla SM,Ettinger RE,Egbert MA

    更新日期:2020-05-01 00:00:00

  • The measure of face-lift patient satisfaction: the Owsley Facelift Satisfaction Survey with a long-term follow-up study.

    abstract:BACKGROUND:Patient satisfaction is a major factor in determining success in aesthetic surgery. To the authors' knowledge, a long-term study measuring patient satisfaction with face-lift surgery has not been published. The authors' study was designed to measure patient satisfaction with the overall experience of a face ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0b013e3181dbc2f0

    authors: Friel MT,Shaw RE,Trovato MJ,Owsley JQ

    更新日期:2010-07-01 00:00:00

  • Suction curettage to remove excess fat for body contouring.

    abstract::We have significantly expanded the use of a lipectomy procedure which heretofore had been restricted to the removal of strictly localized excess fat deposits by curettage through a small incision. By modifying the suction curette and utilizing it for extensive body contouring, we have been successful in correcting rel...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198107000-00012

    authors: Teimourian B,Fisher JB

    更新日期:1981-07-01 00:00:00

  • Staged excision of lentigo maligna and lentigo maligna melanoma: a 10-year experience.

    abstract:BACKGROUND:The treatment of lentigo maligna and lentigo maligna melanoma presents a difficult problem for clinicians. Published guidelines recommend a 5-mm excision margin for lentigo maligna and a 1-cm margin for lentigo maligna melanoma, yet these are often inadequate. The authors' purpose is to report their 10-year ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0b013e3181bcf002

    authors: Bosbous MW,Dzwierzynski WW,Neuburg M

    更新日期:2009-12-01 00:00:00

  • Complications of systemic corticosteroid therapy for problematic hemangioma.

    abstract::Systemic corticosteroid therapy has been used to treat hemangiomas for 30 years; yet, there are no studies of possible complications. We reviewed the database of the Vascular Anomalies Center at the Boston Children's Hospital and gathered information on short- and long-term side effects in children who were given syst...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199911000-00002

    authors: Boon LM,MacDonald DM,Mulliken JB

    更新日期:1999-11-01 00:00:00

  • Global Adverse Event Reports of Breast Implant-Associated ALCL: An International Review of 40 Government Authority Databases.

    abstract:BACKGROUND:Tracking world cases of breast implant-associated anaplastic large cell lymphoma (ALCL) is currently limited to patient registries at a few academic centers, dependent upon patient referral and case reports in the literature. The purpose of this study was to review and compare federal database adverse event ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000003233

    authors: Srinivasa DR,Miranda RN,Kaura A,Francis AM,Campanale A,Boldrini R,Alexander J,Deva AK,Gravina PR,Medeiros LJ,Nast K,Butler CE,Clemens MW

    更新日期:2017-05-01 00:00:00

  • Evolution in minimal-incision palatoplasty: surgical technique and outcomes in 67 consecutive cases.

    abstract:BACKGROUND:Conventional palatoplasty relies on extensive mucosal incisions and isolation of flaps on the palatine vessels to facilitate midline closure and velar reconstruction. This introduces substantial scarring, which has adverse effects on vascularity and growth. The authors have developed a minimally invasive pal...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000000280

    authors: Pan BS,Rapp SJ,Vu A,Uribe-Rivera A,Billmire DA,Gordon CB

    更新日期:2014-07-01 00:00:00

  • Mortality in patients with necrotizing fasciitis.

    abstract:BACKGROUND:The prognostic factors that determine outcome in patients with necrotizing fasciitis remain poorly understood. The aim of this study was to analyze the variables that affect the mortality and morbidity of patients with necrotizing fasciitis and to create a simple method for estimating the probability of mort...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000259040.71478.27

    authors: Golger A,Ching S,Goldsmith CH,Pennie RA,Bain JR

    更新日期:2007-05-01 00:00:00

  • Age-Related Differences in Psychosocial Function of Children with Craniofacial Anomalies.

    abstract:BACKGROUND:Age is a frequent consideration for surgical timing in pediatric craniofacial surgery for optimal psychosocial development. However, systematic evaluations of the effects of age in children under active treatment have not been thoroughly evaluated. METHODS:Ninety-nine patients (age, 8 to 17 years; 46.5 perc...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000003687

    authors: Volpicelli EJ,Pfaff MJ,Hakimi K,Bradley JP,Solem RC,Lee JC

    更新日期:2017-10-01 00:00:00

  • Inhibition of fibroblast-populated collagen lattice contraction by an albumin-bound lipid fraction in human amniotic fluid.

    abstract::Human amniotic fluid inhibits the contraction of fibroblast-populated collagen lattices. Amniotic fluid of 21 weeks' gestation was previously reported to have peak inhibiting activity. An attempt to isolate the inhibitory factor(s) from human amniotic fluid employing a molecular sieving column demonstrated an inhibito...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199801000-00002

    authors: Yager JS,Hugo NE,Ehrlich HP

    更新日期:1998-01-01 00:00:00

  • Rehabilitation of the replanted upper extremity.

    abstract::A concise protocol for the rehabilitation of the upper extremity after replantation is presented with special emphasis in the early mobilization of the hand. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审

    doi:10.1097/00006534-200203000-00027

    authors: Papanastasiou S

    更新日期:2002-03-01 00:00:00

  • Functional reconstruction of a bilateral maxillectomy defect using a fibula osteocutaneous flap with osseointegrated implants.

    abstract::We have achieved functional reconstruction for a bilateral upper alveolar bone, gingival, and palatal defect that has various problems originating from instability of the prosthesis using the fibula osteocutaneous flap with osseointegrated implants. The flap had three bone segments and two skin paddles. The combined b...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199510000-00032

    authors: Nakayama B,Matsuura H,Ishihara O,Hasegawa H,Mataga I,Torii S

    更新日期:1995-10-01 00:00:00

  • A fresh look at the open rhinoplasty technique.

    abstract::Use of the open rhinoplasty technique (ORT) in certain nasal and septal deformities has proven to be an invaluable asset in the rhinoplasty armamentarium of the authors. Primarily owing to the markedly increased exposure with this technique, more accurate assessment of the deformities is possible and additional or imp...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198812000-00006

    authors: Friedman GD,Gruber RP

    更新日期:1988-12-01 00:00:00

  • Single-stage repair of asymmetrical bilateral cleft lip with contralateral lesser form defects.

    abstract:BACKGROUND:Complete or incomplete cleft lip may include a contralateral lesser form of incomplete cleft lip to give rise to an asymmetrical bilateral cleft lip deformity. The principle of simultaneous bilateral cleft repair remains an area of contention with regard to asymmetrical cases, including a lesser form deformi...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0b013e3182402f50

    authors: Bezuhly M,Fisher DM

    更新日期:2012-03-01 00:00:00

  • Melanoma Extirpation with Immediate Reconstruction: The Oncologic Safety and Cost Savings of Single-Stage Treatment.

    abstract:BACKGROUND:The timing of reconstruction following melanoma extirpation remains controversial, with some advocating definitive reconstruction only when the results of permanent pathologic evaluation are available. The authors evaluated oncologic safety and cost benefit of single-stage neoplasm extirpation with immediate...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000002241

    authors: Karanetz I,Stanley S,Knobel D,Smith BD,Bastidas N,Beg M,Kasabian AK,Tanna N

    更新日期:2016-07-01 00:00:00

  • Breast Cancer after Augmentation: Oncologic and Reconstructive Considerations among Women Undergoing Mastectomy.

    abstract:BACKGROUND:Breast augmentation with subglandular versus subpectoral implants may differentially impact the early detection of breast cancer and treatment recommendations. The authors assessed the impact of prior augmentation on the diagnosis and management of breast cancer in women undergoing mastectomy. METHODS:Breas...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000003342

    authors: Cho EH,Shammas RL,Phillips BT,Greenup RA,Hwang ES,Hollenbeck ST

    更新日期:2017-06-01 00:00:00

  • "Donor" muscle structure and function after end-to-side neurorrhaphy.

    abstract::End-to-end nerve coaptation is the preferred surgical technique for peripheral nerve reconstruction after injury or tumor extirpation. However, if the proximal nerve stump is not available for primary repair, then end-to-side neurorrhaphy may be a reasonable alternative. Numerous studies have demonstrated the effectiv...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-200103000-00021

    authors: Cederna PS,Kalliainen LK,Urbanchek MG,Rovak JM,Kuzon WM Jr

    更新日期:2001-03-01 00:00:00

  • Slowed growth of cultured fibroblasts from human radiation wounds.

    abstract::To study radiation effect separate from the microcirculation, fibroblasts were cultured from four patients with radiation wounds. Cells could be grown from irradiated tissue near the ulcer and from control normal tissue, but no cells could be cultured from the ulcers. The ability of radiation-treated fibroblasts to at...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198810000-00019

    authors: Rudolph R,Vande Berg J,Schneider JA,Fisher JC,Poolman WL

    更新日期:1988-10-01 00:00:00

  • Evolution of Bilateral Free Flap Breast Reconstruction over 10 Years: Optimizing Outcomes and Comparison to Unilateral Reconstruction.

    abstract:BACKGROUND:There is an increasing trend for contralateral prophylactic mastectomy, but studies focusing on bilateral free flap breast reconstruction are lacking. METHODS:A retrospective review was performed of all bilateral free flap breast reconstructions performed from 2000 to 2010. RESULTS:Overall, 488 patients un...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000001233

    authors: Chang EI,Chang EI,Soto-Miranda MA,Zhang H,Nosrati N,Ghali S,Chang DW

    更新日期:2015-06-01 00:00:00