Abstract:
BACKGROUND:Surgical treatment of injuries with loss of skin tissue has improved significantly with the advent of regenerative medicine and tissue bioengineering, and the use of stem cells and dermal substitutes. The success of tissue regeneration depends on optimal local vascularization and the successful integration of the artificial skin. The present study combines the use of autologous adipose-derived stem cells with a commercially available dermal substitute (Integra) for skin regeneration. METHODS:Adipose-derived stem cells were isolated from the inguinal region of eight Sprague-Dawley adult rats, seeded onto a piece of dermal substitute for 48 hours, and then implanted into the same rat, followed by comparison of the evolution with a contralateral implant without adipose-derived stem cells. After 1, 2, and 3 weeks of regeneration in vivo, implants were removed for histologic evaluation. RESULTS:Adipose-derived stem cells adhere properly to the dermal matrix, and autologous tissue integration in the rat was good. The histologic evaluation showed that adipose-derived stem cells significantly increased microvascular density (7.7 ± 0.6 percent versus 5.3. ± 0.5 percent, as assessed by immunohistochemical staining of factor VIII) and the synthesis of collagen type I (24 ± 3 percent versus 16 ± 2 percent, as assessed by Sirius red staining). Although there was greater epithelialization on the side treated with adipose-derived stem cells (2530 ± 939 μm versus 1911 ± 763 μm), as assessed by anticytokeratin immunohistochemistry staining 34βE12, this difference was not statistically significant. CONCLUSIONS:The authors' data suggest that the seeding of adipose-derived stem cells onto a dermal substitute improves skin regeneration and tissue integration by increasing vascularity and collagen synthesis. Further studies are necessary to achieve complete epithelialization with the use of adipose-derived stem cells.
journal_name
Plast Reconstr Surgjournal_title
Plastic and reconstructive surgeryauthors
Meruane MA,Rojas M,Marcelain Kdoi
10.1097/PRS.0b013e3182547e04subject
Has Abstractpub_date
2012-07-01 00:00:00pages
53-63issue
1eissn
0032-1052issn
1529-4242pii
00006534-201207000-00013journal_volume
130pub_type
杂志文章abstract::Craniofacial osteotomies have by convention been bilamellar translocations of the entire substance of the dysmorphic bone. This approach limits the surgeon by reducing the stable bone mass available for fixation, creating dependence on concave surfaces. Most important, it changes the bony topography that determines th...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract:BACKGROUND:Tools to quantify layperson assessments of facial palsy are lacking. In this study, artificial intelligence was applied to develop a proxy for layperson assessments, and compare sensitivity to existing outcome measures. METHODS:Artificially intelligent emotion detection software was used to develop the emot...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0000000000005865
更新日期:2019-08-01 00:00:00
abstract::We present a series of 70 patients who have had bilateral subcutaneous mastectomies with immediate placement of implants under the intact pectoralis major and upper part of the serratus anterior. Twenty-two of these patients have had a simultaneous mastopexy, with a free nipple graft. All 70 patients have both of thei...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-197811000-00006
更新日期:1978-11-01 00:00:00
abstract::Based on anatomic, dye injection, and angiography observations since 1984, 72 patients with tissue defects of the foot (due to injury or surgery) have been reconstructed using a reverse-flow posterior tibial artery island flap. In the 72 patients, etiology has included extensive crushing (40 patients), burn scars (10 ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Virtual surgical planning and computer-aided design and computer-aided modeling are promising technologies with a growing trend in contemporary head and neck reconstruction. Segmental mandibular reconstruction with the osteoseptocutaneous fibula flap is one of the fields in which these technologies are increasingly us...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,评审
doi:10.1097/01.prs.0000479998.49928.71
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Extensive spinal neoplasms are difficult to manage. Following resection, arthrodesis of the spine can be performed with instrumentation, but this often fails in the setting of radiation therapy. Use of the free fibula flap for anterior spinal fusion to correct deformities has been described in multiple studi...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0b013e31820cf4a6
更新日期:2011-05-01 00:00:00
abstract::Based on the 14 free flap transfers we present with full survival, we recommend peroperative magnification angiography of both the recipient and donor sites. In our hands, this type of preoperative assessment seems to increase the margin of safety. ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-197910000-00007
更新日期:1979-10-01 00:00:00
abstract::A primary gastrocnemius transposition flap is a useful technique for prosthetic coverage following extensive soft-tissue and bone resection for sarcomas of the knee joint. The gastrocnemius transposition flap is also a useful secondary procedure to treat local complications following attempted limb-sparing surgery. Th...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-198405000-00004
更新日期:1984-05-01 00:00:00
abstract:BACKGROUND:This study was undertaken to determine contemporary surgical management of bilateral cleft lip. METHODS:A survey was sent to North American cleft surgeons. Variables included number of bilateral cleft lip repairs per year, surgical specialty, and methods of nasolabial correction. The authors analyzed possib...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,多中心研究
doi:10.1097/PRS.0b013e31824ecbd3
更新日期:2012-06-01 00:00:00
abstract::A study on the effects of iontophoretic administration of calcitonin gene-related peptide and vasoactive intestinal polypeptide on the healing of venous stasis ulcers of the extremities was carried out on 66 patients. Two randomized groups of patients were compared, one receiving standard treatment plus iontophoresis ...
journal_title:Plastic and reconstructive surgery
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00006534-199801000-00015
更新日期:1998-01-01 00:00:00
abstract::The biocompatibility of prosthetic mesh is dependent on a number of physicochemical properties that ultimately incite an optimal foreign body response. The magnitude and character of the foreign body response directly affect the clinical success of the hernia repair, with too little scar resulting in bulge or hernia r...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,评审
doi:10.1097/PRS.0000000000003930
更新日期:2018-01-01 00:00:00
abstract::The purpose of this study was to compare the sensitivity of a 20-mHz ultrasonic Doppler device to detect microvascular thrombosis placed on the vein with that placed on the artery. A feasibility study in animals preceded a comparative clinical study in patients. Six rabbits were used to develop a bilateral hind limb p...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-199401000-00024
更新日期:1994-01-01 00:00:00
abstract::The purpose of this study was to evaluate the efficacy of a modified mattress suture technique in septal anterior deviation correction and to compare it with the Hinderer technique. This was a randomized, controlled, single-blind clinical trial. Fifty patients with anterior septal deviation were assigned to one of two...
journal_title:Plastic and reconstructive surgery
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/01.prs.0000138816.48312.ad
更新日期:2004-11-01 00:00:00
abstract::The external ear of the rat is an excellent model for practicing microsurgical dissection and for the refinement of microvascular anastomoses, techniques that are crucial for microvascular en bloc tissue transfer and replantation. Preparation of the rat ear for replantation requires familiarity with the vascular anato...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-199005000-00020
更新日期:1990-05-01 00:00:00
abstract:BACKGROUND:Tobacco use remains a persistent risk factor in elective plastic surgery. Although nicotine is thought to increase complications, which procedures are affected and the reliability of patient-provided histories remain poorly defined. The authors sought to examine nicotine use and its impact on outcomes. METH...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0b013e318277886a
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Bionic limbs require sensitive, durable, and physiologically relevant bidirectional control interfaces. Modern central nervous system interfacing is high risk, low fidelity, and failure prone. Peripheral nervous system interfaces will mitigate this risk and increase fidelity by greatly simplifying signal int...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0b013e3181f61848
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:Two commonly used methods of digital nerve block with local anesthetic are the two-injection dorsal technique and the single-injection volar subcutaneous technique. The authors compared these two digital block techniques with respect to local anesthetic injection pain and recipient preference of anesthetic t...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,随机对照试验
doi:10.1097/01.prs.0000237016.00941.96
更新日期:2006-10-01 00:00:00
abstract::Marked improvement in the aesthetic appearance of scars and skin grafts of the hair-bearing facial areas of male patients can be achieved by camouflage with punctate tattooing. The technique is easy and simple to learn. The procedure is performed under local anesthesia and on an outpatient basis. ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-199308000-00027
更新日期:1993-08-01 00:00:00
abstract::A number of materials, both biologic and alloplastic, have been used for nasal augmentation. Although biologic bone and cartilage grafts are associated with lower infection rates, they are also associated with long-term resorption and donor-site morbidity. Alloplastic materials, in particular silicone, have been assoc...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-199809040-00052
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:Fat grafting may be beneficial in prosthetic reconstruction of irradiated tissues, but there is a paucity of data on the complication rates associated with this clinical scenario. METHODS:All consecutive patients who had undergone fat grafting and prosthetic reconstruction from 2010 to 2013 were enrolled. V...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0000000000002916
更新日期:2017-01-01 00:00:00
abstract::This clinical study was undertaken to demonstrate the feasibility of free transfer of undescended human testes with microvascular anastomoses. We have performed 4 microvascular free transfers of such testes in children aged 5, 6, and 8 years, with follow-up periods of one to three years. All 4 testes have remained cli...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-197901000-00007
更新日期:1979-01-01 00:00:00
abstract::This pilot study investigated the feasibility of Google Glass to assist visualization of fluoroscopic images during percutaneous pinning of hand fractures. Cadavers were used to compare total time to pin each fracture and total number of radiographs per fracture from a mini C-arm. A FluoroScan monitor was used for rad...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0000000000001453
更新日期:2015-08-01 00:00:00
abstract::Split-thickness nail bed grafting is the accepted method of treatment for injuries involving loss of nail bed tissue. The nail bed of the great toe may be used without donor-site morbidity, and nail bed grafting may be combined with other procedures for fingertip reconstruction. A case of fingertip avulsion injury wit...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-198803000-00023
更新日期:1988-03-01 00:00:00
abstract:BACKGROUND:Despite well-established correlation of postmastectomy radiotherapy and surgical complications in breast reconstruction, its impact on patient reported outcomes is less clear. We sought to determine the effect of postmastectomy radiotherapy on patient reported outcomes throughout the breast reconstruction pr...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0000000000004105
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:Restoration of biomechanical strength following surgical reconstruction of tendon or ligament insertion tears is challenging because these injuries typically heal as fibrous scars. The authors hypothesize that injuries at the tendon-bone interface would benefit from reconstruction with decellularized composi...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/01.prs.0000436823.64827.a0
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Acute compartment syndrome of the hand is an emergency requiring prompt surgical decompression. This cadaveric study aims to identify the myofascial compartments of the hand by detailed dissection and direct visualization, to provide an accurate anatomical basis for surgical decompression. METHODS:Fourteen ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0b013e3181956538
更新日期:2009-02-01 00:00:00
abstract::The rectus abdominis muscle has been used in reconstructive surgery as a superiorly and an inferiorly based pedicle flap as well as free flap. Since flap necrosis is unusual, the primary morbidity of the harvesting is donor-site complications, including infections, seromas, poor cosmesis, and hernias. Minimally invasi...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-199601000-00034
更新日期:1996-01-01 00:00:00
abstract::Not only is the long temporal vertical flap not centered on the temporosuperficial artery, but it is also countercurrent to this artery. The difficulties of its design are largely compensated for by the natural aspect of the anterior area once achieved. It must be emphasized that all techniques of transplantation can ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-198411000-00007
更新日期:1984-11-01 00:00:00
abstract::The external fixater has proven to be an effective method of providing immobilization in pedicle-flap surgery. In certain instances, when the pedicle will permit some degree of mobility, limited-motion fixation eliminates the common disadvantages of rigid fixation. On this basis, we recommend the use of a ball-joint a...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-198308000-00027
更新日期:1983-08-01 00:00:00
abstract:BACKGROUND:Although multiple acellular dermal matrix sources exist, it is unclear how its processing impacts complication rates. The authors compared complications between two preparations of human cadaveric acellular dermal matrix (freeze dried and ready-to-use) in immediate tissue expander breast reconstruction to an...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0000000000004323
更新日期:2018-05-01 00:00:00