Abstract：
:This study analysed 330 environmental substrates from three dairy farms for the occurrence, drug resistance and the genetic mutations of MTBC (Mycobacterium tuberculosis complex) in Eastern Cape, South Africa using PCR, while the Genotype MTBDRplus assay was used for drug susceptibility and genetic mutations analyses. About 17% (55/330) of the samples were positive for MTBC at 16.7% (water), 13.3% (soil) and 20% (hayfeed). Isoniazid resistance was detected in 47.3% (26/55) of the samples while 16.4% (9/55) were multidrug-resistant. Genetic mutations were detected on the rpoB gene (resistance to rifampicin) with frequencies ranging from 53.6% (D516V) to 21.4% (H526D), while mutations on the katG and inhA genes (resistance to isoniazid) ranged between 14.3% and 80%. Incidents of diverse genetic mutations in the environmental matrices suggest possible resistance to other anti-TB drugs not assayed in this study and emphasizes the need for continuous monitoring of drug resistance patterns for timely detection and control of new clonal groups of MTBC.
journal_name
Int J Environ Health Resjournal_title
International journal of environmental health researchauthors
Ntloko A,Adefisoye MA,Green Edoi
10.1080/09603123.2019.1642458subject
Has Abstractpub_date
2021-03-01 00:00:00pages
215-224issue
2eissn
0960-3123issn
1369-1619journal_volume
31pub_type
杂志文章abstract：:A national survey of local authorities was carried out to ascertain what information they collected on reported cases of intestinal infectious disease and why. As a result of the survey, and a review of national objectives, two data collection forms designed to satisfy both local and national objectives were piloted. ...
journal_title：International journal of environmental health research
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