Abstract:
:Identifying the causes of high fever syndromes such as Kawasaki disease (KD) remains challenging. To investigate pathogen exposure signatures in suspected pathogen-mediated diseases such as KD, we performed immunoglobulin (Ig) profiling using a next-generation sequencing method. After intravenous Ig (IVIG) treatment, we observed disappearance of clonally expanded IgM clonotypes, which were dominantly observed in acute-phase patients. The complementary-determining region 3 (CDR3) sequences of dominant IgM clonotypes in acute-phase patients were commonly observed in other Ig isotypes. In acute-phase KD patients, we identified 32 unique IgM CDR3 clonotypes shared in three or more cases. Furthermore, before the IVIG treatment, the sums of dominant IgM clonotypes in IVIG-resistant KD patients were significantly higher than those of IVIG-sensitive KD patients. Collectively, we demonstrate a novel approach for identifying certain Ig clonotypes for potentially interacting with pathogens involved in KD; this approach could be applied for a wide variety of fever-causing diseases of unknown origin.
journal_name
Hum Mol Genetjournal_title
Human molecular geneticsauthors
Ko TM,Kiyotani K,Chang JS,Park JH,Yin Yew P,Chen YT,Wu JY,Nakamura Ydoi
10.1093/hmg/ddy176subject
Has Abstractpub_date
2018-08-01 00:00:00pages
2671-2677issue
15eissn
0964-6906issn
1460-2083pii
4996736journal_volume
27pub_type
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