当前位置： SCI文献检索 > ACTA NEUROCHIRURGICA期刊下所有文献 > Bacterial colonisation of suture material after routine neurosurgical procedures: relevance for wound infection.

Bacterial colonisation of suture material after routine neurosurgical procedures: relevance for wound infection.

Abstract：

BACKGROUND:Wound healing impairment is a serious problem in surgical disciplines which may be associated with chronic morbidity, increased cost and patient discomfort. Here we aimed to investigate the relevance of bacterial colonisation on suture material using polymerase chain reaction (PCR) to detect and taxonomically classify bacterial DNA in patients with and without wound healing problems after routine neurosurgical procedures. METHODS:Repeat surgery was performed in 25 patients with wound healing impairment and in 38 patients with well-healed wounds. To determine the presence of bacteria, a 16S rDNA-based PCR detection method was applied. Fragments of 500 bp were amplified using universal primers which target hypervariable regions within the bacterial 16S rRNA gene. Amplicons were separated from each other by single-strand conformation polymorphism (SSCP) analysis, and finally classified using Sanger sequencing. RESULTS:PCR/SSCP detected DNA of various bacteria species on suture material in 10/38 patients with well-healed wounds and in 12/25 patients with wound healing impairment including Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes and Escherichia coli. Microbiological cultures showed bacterial growth in almost all patients with wound healing impairment and positive results in PCR/SSCP (10/12), while this was the case in only one patient with a well-healed wound (1/10). CONCLUSIONS:Colonisation of suture material with bacteria occurs in a relevant portion of patients with and without wound healing impairment after routine neurosurgical procedures. Suture material may provide a nidus for bacteria and subsequent biofilm formation. Most likely, however, such colonisation of sutures is not a general primer for subsequent wound infection.

journal_name

Acta Neurochir (Wien)

journal_title

Acta neurochirurgica

authors

Hong B,Winkel A,Ertl P,Stumpp SN,Schwabe K,Stiesch M,Krauss JK

doi

10.1007/s00701-017-3404-9

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

497-503

issue

eissn

0001-6268

issn

0942-0940

pii

10.1007/s00701-017-3404-9

journal_volume

160

pub_type

杂志文章

在线工具

Bacterial colonisation of suture material after routine neurosurgical procedures: relevance for wound infection.