Abstract:
:This review provides an update on the promises and pitfalls when using in vitro bioassays to evaluate beneficial and adverse health effects of botanicals and botanical preparations. Important issues addressed in the paper are: (i) the type of assays and biological effects available; (ii) false-positives, false-negatives and confounding factors; (iii) matrix and combination effects; (iv) extrapolation of in vitro data to the in vivo situation; (v) when (not) to use bioassays; and (vi) identification of active constituents. It is concluded that in vitro bioassays provide models to detect beneficial as well as adverse activities, but that linking these observations to individual ingredients and extrapolations to the in vivo situation is more complicated than generally anticipated.
journal_name
Drug Discov Todayjournal_title
Drug discovery todayauthors
Prinsloo G,Papadi G,Hiben MG,de Haan L,Louisse J,Beekmann K,Vervoort J,Rietjens IMCMdoi
10.1016/j.drudis.2017.05.002subject
Has Abstractpub_date
2017-08-01 00:00:00pages
1187-1200issue
8eissn
1359-6446issn
1878-5832pii
S1359-6446(17)30024-7journal_volume
22pub_type
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