Abstract：
:Peripheral blood mononuclear cells (PBMNC) from 23 healthy subjects and 39 patients with B-cell chronic leukaemia (B-CLL) were assayed for ribonuclease H activity using as substrate the filter-immobilized synthetic homopolymer hybrid 3H-poly(rA):poly(dT). In 69% of the leukaemia patients examined enzyme activities were above those estimated in cells from the healthy controls. The mean enzyme levels for the normal and the leukaemic samples group were (per cent substrate hydrolysis): 8.1 +/- 8.9 and 58.7 +/- 40.8, respectively, their difference being statistically highly significant (P less than 0.0001). This result does not represent homogeneity within the cells but is due to a subclass of cells within the leukaemic clone containing the enzyme, thus contributing through pool size fluctuation to the wide variations of enzyme activity observed among the patients. These cells containing high activity could not be identified with either the prolymphocytes or the large lymphocytes. The activity of ribonuclease H in the examined CLL patients correlated with disease stage (Binet) (P = 0.011) and appeared to serve as a sensitive indicator of disease progression when compared with a number of other known prognostic parameters.
journal_name
Br J Haematoljournal_title
British journal of haematologyauthors
Papaphilis AD,Kamper EF,Kattamis C,Pangalis GAdoi
10.1111/j.1365-2141.1988.tb02486.xsubject
Has Abstractpub_date
1988-11-01 00:00:00pages
301-6issue
3eissn
0007-1048issn
1365-2141journal_volume
70pub_type
杂志文章abstract：:MPLW515L/K and JAK2V617F can co-exist in myelofibrosis with myeloid metaplasia (MMM). The chronology of clonal emergence was studied in three such cases using serially stored bone marrow. At diagnosis, a major MPL515 mutant clone was accompanied by a minor JAK2V617F clone in all three instances. At 25 time points over...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.2006.06348.x
更新日期：2006-12-01 00:00:00
abstract：:Mantle cell lymphoma (MCL) is an incurable mature B cell proliferation, combining the unfavourable clinical features of aggressive and indolent lymphomas. The blastic variant of MCL has an even worse prognosis and new treatment options are clearly needed. We analysed the effects of BL22, an immunotoxin composed of the...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.2009.07939.x
更新日期：2010-01-01 00:00:00
abstract：:In the present study, we aimed to identify distinct structural and numerical chromosomal aberrations in peripheral blood B cells of patients with myeloma and monoclonal gammopathy of undetermined significance (MGUS), which reflect changes thought to occur at different stages of the disease process. Peripheral blood fr...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.2002.03529.x
更新日期：2002-06-01 00:00:00
abstract：:This study reports the characterization of a spontaneous lymphoblastoid cell line (LCL) raised from the peripheral blood of a patient with Kostmann's congenital neutropenia. The LCL was composed of EBV-infected polyclonal B cells and displayed surface markers and pattern of growth in vitro typical of normal LCLs. The ...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.1993.tb03209.x
更新日期：1993-12-01 00:00:00
abstract：:Peripheral blood progenitor cell (PBPC) mobilization was evaluated in 53 patients receiving the high-dose sequential (HDS) regimen: 27 had non-Hodgkin's lymphoma or Hodgkin's disease, primary refractory or at first relapse, 26 had non-Hodgkin's lymphoma at diagnosis. Mobilization was assessed following either 7 g/m2 c...
journal_title：British journal of haematology
pub_type： 临床试验,杂志文章
doi：10.1046/j.1365-2141.1997.3433157.x
更新日期：1997-10-01 00:00:00
abstract：:Lymphoproliferative diseases of large granular lymphocytes (LDGL) may arise from either CD3+ T cells or CD3- natural killer (NK) cells. LDGL with clonal proliferation of large granular lymphocytes (LGL) is defined as LGL leukaemia. The number of patients with NK-LGL leukaemia reported is limited and the pathogenesis o...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.1998.01109.x
更新日期：1998-12-01 00:00:00
abstract：:We report a series of 20 patients with the myelodysplastic syndrome (MDS) each with a coexistent lymphoid or plasmacytic neoplasm. In none of the patients did chemotherapy play a part in the pathogenesis. The possible reasons for the coincidence of these conditions are discussed. The frequency and variety of associate...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.1986.tb07505.x
更新日期：1986-05-01 00:00:00
abstract：:Idiopathic thrombotic thrombocytopenic purpura (TTP) is characterized by frequent recurrences. Effective screening for relapses will enable intervention prior to overt episodes of TTP. The present study used a modified assay to detect ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type 1 motif, 13) ac...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.2008.07107.x
更新日期：2008-05-01 00:00:00
abstract：:A prospective study was undertaken to determine the effectiveness of an empirical antibiotic therapy regimen in 34 neutropenic patients undergoing bone marrow transplantation or remission-induction chemotherapy for leukaemia. Throughout the study period a total of 90 pyrexial episodes were monitored in which organisms...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:Clonal heterogeneity in multisited or recurrent lymphoid neoplasms is a phenomenon that has been increasingly studied in recent years. However, in mucosa-associated lymphoid tissue (MALT) lymphomas it remains largely unexplored. Patients diagnosed at our institution with multisited MALT lymphoma, from January 2009 to ...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/bjh.16717
更新日期：2020-05-23 00:00:00
abstract：:The Brown Norwegian rat transplanted with promyelocytic leukaemic cells (BNML) has been used as a model for human acute myeloid leukaemia. We have previously shown that both the blood supply to the bone marrow and the metabolic rate decrease in relation to the leukaemic development in these rats. Here we have investig...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.1998.00801.x
更新日期：1998-07-01 00:00:00
abstract：:The success of genetically engineered T cells that express chimeric antigen receptors (CARTs) has been a momentous step forward in harnessing the potent cancer fighting abilities of the immune system. The efficacy seen in relapsed/refractory (r/r) acute lymphoblastic leukaemia (ALL), not only by inducing remission, bu...
journal_title：British journal of haematology
pub_type： 杂志文章,评审
doi：10.1111/bjh.14475
更新日期：2017-04-01 00:00:00
abstract：:Heterozygous factor XI (FXI) deficiency is sometimes associated with a significant bleeding tendency. Fresh frozen plasma of FXI concentrates are the mainstay of treatment in patients with a clear bleeding history, especially prior to surgery. However, these treatments are not completely free of risk. Furthermore, thr...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.1996.d01-1758.x
更新日期：1996-07-01 00:00:00
abstract：:It is the capacity to generate thrombin, and the enzymatic work that thrombin does, that determines blood coagulability. Therefore, measurement of the enzymatic work potential of thrombin provides a method for quantifying the composite effect of the multiple factors that determine coagulation capacity. The application...
journal_title：British journal of haematology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2141.2005.05612.x
更新日期：2005-09-01 00:00:00
abstract：:Immunoglobulin kappa (Igkappa) gene recombinations can be used - similarly to IgH rearrangements - as clonal markers in B-lineage leukaemias. Based on the extensive junctional diversity, these rearrangements represent valuable targets for the analysis of minimal residual disease (MRD). In order to provide a simple met...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.1999.01557.x
更新日期：1999-08-01 00:00:00
abstract：:Recent findings have indicated the importance of factor V (FV) in causing resistance to activated protein C (APC) in a high proportion of patients with venous thrombosis. This prompted us to investigate whether resistance could be due to defective inactivation of FVa by APC. Consequently, we amplified a 3.2 kb fragmen...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.1994.tb05005.x
更新日期：1994-09-01 00:00:00
abstract：:The phenotypic expression and functional capacity of natural killer (NK) T-lymphocytes (E+, OKT3+) were analysed in a series of untreated patients with B-cell chronic lymphocytic leukaemia (B-CLL). The mean value of NK activity of B-CLL T-lymphocytes, tested against the K562 cell line, was significantly depressed (P l...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.1984.tb03998.x
更新日期：1984-11-01 00:00:00
abstract：:Twenty adult patients with high-risk leukaemia underwent allogeneic haemopoietic stem cell transplantation after melphalan, busulphan and total body irradiation followed by short-term methotrexate and low-dose cyclosporine or tacrolimus. Three patients developed veno-occlusive disease and no patient developed renal dy...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.1999.01424.x
更新日期：1999-06-01 00:00:00
abstract：:We performed a retrospective analysis of outcome in 45 patients with multiple myeloma receiving unrelated donor stem cell transplants (UD-SCT) in the UK between 1993 and 2002; 17 received myeloablative conditioning regimens and 28 received reduced intensity conditioning (RIC) protocols. Forty patients received pretran...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.2003.04714.x
更新日期：2003-12-01 00:00:00
abstract：:Twenty-six members of four generations of one family in which a man was diagnosed in 1961 as having von Willebrand's disease (vWD) have been studied. Subtype IIB vWD and autosomal dominant inheritance was identified in 19 individuals with bleeding signs varying in severity and frequency. The absence of high molecular ...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.1988.tb02406.x
更新日期：1988-08-01 00:00:00
abstract：:Haematological characteristics have been compared in 29 subjects with heterozygous beta0 thalassaemia and in 33 subjects with heterozygous beta+ thalassaemia, identified by the type of sickle cell-beta thalassaemia among close relatives, in a Jamaican Negro population. Total haemoglobin, MCV and MCH were significantly...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.1977.tb00636.x
更新日期：1977-06-01 00:00:00
abstract：:Podocalyxin-like protein (PCLP) is a sialomucin-type membrane protein structurally related to CD34 and endoglycan. It was first described in glomerular podocytes and endothelial cells. In mice, PCLP is present in haemangioblasts, and in both chicken and mice it is a marker of early haematopoietic stem cells and lineag...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.2004.04840.x
更新日期：2004-03-01 00:00:00
abstract：:Primary ocular lymphoma (POL), a lymphoma of the globe, is a restricted form of primary central nervous system lymphoma (PCNSL) that often progresses to the brain and meninges; frequently it is misdiagnosed until central nervous system (CNS) lymphoma develops. The optimal treatment has not yet been identified. We retr...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1111/j.1365-2141.2004.05028.x
更新日期：2004-07-01 00:00:00
abstract：:Accumulating evidence indicates that a graft-vs.-myeloma effect (GVM) and its associated clinical remission of the disease can be induced by donor lymphocyte infusion in myeloma patients who have relapsed after allogeneic bone marrow transplantation. Although it is believed that GVM is induced by allospecific T cells,...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.2001.02531.x
更新日期：2001-02-01 00:00:00
abstract：:Sickle cell disease (SCD) is increasingly appreciated as an inflammatory condition associated with alterations in immune phenotype and function. In this cross-sectional study we performed a multiparameter analysis of 18 immune markers in 114 paediatric SCD patients divided by treatment group [those receiving hydroxycr...
journal_title：British journal of haematology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1111/bjh.13326
更新日期：2015-05-01 00:00:00
abstract：:The PFA-100(R) (PFA) diagnostic system for the detection of platelet dysfunction was evaluated to determine reference ranges in a normal population. The PFA determines the primary haemostasis capacity (PHC) of anticoagulated whole blood, expressed by the system's closure time (CT). In this study the CT reference range...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.1999.01660.x
更新日期：1999-09-01 00:00:00
abstract：:The 'high and low responder phenomenon' of monocyte tissue factor (MTF) activity has been attributed to effects on monocytes by granulocytes, platelets and lipopolysaccharide (LPS). To study the possible contribution of plasma to the high and low responder phenomenon, we measured the MTF activity in isolated cryoprese...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.2001.02545.x
更新日期：2001-01-01 00:00:00
abstract：:The highly conserved 350-bp major regulatory element HS-40 (or alphaMRE) upstream of the human alpha-globin gene cluster is involved in the regulation of alpha-globin gene expression. The study of alphaMRE differences between human populations and the evolution of alphaMRE sequences in mammals may lead to a better und...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.2002.03917.x
更新日期：2002-12-01 00:00:00
abstract：:The main objective of the present study was to determine the role of CD34+ cell subsets in the haemopoietic recovery of children undergoing peripheral blood stem cell transplantation. For this purpose, 38 leukaphereses from 33 children with malignancies mobilized with G-CSF were analysed. Using dual-colour flow cytome...
journal_title：British journal of haematology
pub_type： 杂志文章
doi：10.1046/j.1365-2141.1998.00662.x
更新日期：1998-04-01 00:00:00
abstract：:Non-endemic Burkitt lymphoma (BL) is a rare germinal centre B-cell-derived malignancy with the genetic hallmark of MYC gene translocation and with rapid tumour growth as a distinct clinical feature. To investigate treatment outcomes, loss of lifetime and relapse risk in adult BL patients treated with intensive immunoc...
journal_title：British journal of haematology
pub_type： 杂志文章,多中心研究
doi：10.1111/bjh.16425
更新日期：2020-05-01 00:00:00
