Abstract:
:Determination of thyroid-stimulating hormone (TSH) level in serum or plasma is defined as a sensitive method for the diagnosis of hyperthyroidism and hypothyroidism and also in many diseases thought to be related to TSH levels. In this study, a novel simple impedimetric immunosensor based on polyamidoamine dendrimer was developed. Anti TSH antibody was immobilized on the gold electrode by using cysteamine self-assembled monolayer strategy. In constructing the immunosensor, a polyamidoamine dendrimer was used to increase the surface area in which Antı-TSH was immobilized and glutaraldehyde was used as a cross-linker. After each immobilization step, the electrode surface was monitored by electrochemical impedance spectroscopy, cyclic voltammetry, scanning electron microscopy and energy-dispersive X-ray spectroscopy techniques and optimization studies were performed. The reproducibility, repeatability, linearity and sensitivity of the immunosensor were examined. Also, the interference experiments for glucose, salts and proteins in serum were performed. The limit of detection and limit of quantification values of the proposed immunosensor were 0.026 mIUL-1 and 0.086 mIUL-1, respectively and it was able to detect the amount of TSH within a linear range of 0.1-0.6 mIUL-1.
journal_name
Artif Cells Nanomed Biotechnoljournal_title
Artificial cells, nanomedicine, and biotechnologyauthors
Ozcan HM,Aydin UDdoi
10.1080/21691401.2020.1867153keywords:
["Thyroid-stimulating hormone","cyclic voltammetry","electrochemical impedance spectroscopy","immunosensor","self-assembled monolayer"]subject
Has Abstractpub_date
2021-12-01 00:00:00pages
61-70issue
1eissn
2169-1401issn
2169-141Xjournal_volume
49pub_type
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