Development of a novel methodology for in vivo quantification of N/O/S-containing polycyclic aromatic hydrocarbons located on the epidermis of mangrove roots using graphene quantum dots as a fluorescence quencher.

Abstract:

:A novel approach for in vivo determination of typical N/O/S-containing PAHs located on the epidermis of mangrove roots was developed using graphene quantum dots (GQDs) as a fluorescence quencher. The decreasing fluorescence intensity from GQDs was attributed to the amount of N/O/S-containing PAHs introduced onto the epidermis of mangrove roots. The linear ranges of the proposed method were 10.3-980ngg-1, 9.5-1350ngg-1 and 7.8-1200ngg-1 for DBF, DBT and CAR located on the epidermis of K. obovata roots, respectively. This method was also shown to be valid for quantifying the N/O/S-containing PAHs on the root epidermis in the presence of heavy metal (10mmolL-1) and dissolved organic matter (1mgL-1 C). Moreover, the death rates of epidermal cells were almost unchanged (p>0.05) after acquiring the fluorescence spectra, which is superior to the previously reported LITRF method with which the cell death rates increased to 42.6%.

journal_name

Mar Pollut Bull

authors

Li R,Wang S,Wang Y,Yu K

doi

10.1016/j.marpolbul.2017.12.040

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

424-428

eissn

0025-326X

issn

1879-3363

pii

S0025-326X(17)31073-1

journal_volume

127

pub_type

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