Abstract:
BACKGROUND:In obtaining human tenocytes for tendon tissue engineering, a low proliferation rate and phenotype loss during passaging is a problem. It was the authors' aim to evaluate the influence of vascular endothelial growth factor (VEGF) on human tenocyte growth and gene expression. METHODS:Human tenocytes were exposed to human VEGF in various concentrations (5, 10, and 20 ng/ml) for 5 days. Cell proliferation was counted and expression of tendon-related genes was analyzed. RESULTS:Tenocyte count was 1.4 × 10(5)/ml, 2.7 × 10(5)/ml, 2.3 × 10(5)/ml, and 3.7 × 10(5)/ml for 0, 5, 10, and 20 ng/ml VEGF, respectively. Expression of Col1 was up-regulated 6.4 ± 4.2-fold, 60.1 ± 21.6-fold, and 15.8 ± 10.2-fold for 5, 10, and 20 ng/ml VEGF; all differences were significant with p < 0.05. Col3 was down-regulated to 0.2 ± 0.1-fold, 0.3 ± 0.1-fold, and 0.1 ± 0.03-fold for 5, 10, and 20 ng/ml VEGF; all differences were significant. Eln was up-regulated 2.3 ± 1.7-fold, 25.5 ± 10.9-fold, and 16.6 ± 9.0-fold for 5, 10, and 20 ng/ml VEGF; differences were significant for 10 and 20 ng/ml VEGF. TSC was down-regulated to 0.3 ± 0.1-fold and 0.3 ± 0.1-fold for 5 and 20 ng/ml VEGF; differences were significant for 5 and 20 ng/ml. SCX was up-regulated to 31.3 ± 8.5-fold, 49.1 ± 23.4-fold, and 20.9 ± 9.5-fold for 5, 10, and 20 ng/ml VEGF; all changes were significant. CONCLUSIONS:VEGF enhances proliferation and expression of tendon-related genes in human tenocytes. It could therefore be a useful addition for tenocyte cultivation.
journal_name
Plast Reconstr Surgjournal_title
Plastic and reconstructive surgeryauthors
Kraus A,Sattler D,Wehland M,Luetzenberg R,Abuagela N,Infanger Mdoi
10.1097/PRS.0000000000004920subject
Has Abstractpub_date
2018-11-01 00:00:00pages
1240-1247issue
5eissn
0032-1052issn
1529-4242pii
00006534-201811000-00024journal_volume
142pub_type
杂志文章abstract::Implicit to any surgeon's abdominal wall reconstruction (AWR) practice should be the review of results after operations have been performed. This allows surgeons to ensure they are delivering high-quality care to patients. In addition, systematic collection of data centered around AWR greatly facilitates research and ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0000000000004878
更新日期:2018-09-01 00:00:00
abstract::Early surgery for isolated craniosynostosis is designed to improve morphology, to prevent functional disturbances, and equally important, to enhance the psychosocial development of the child. As the first of a two-part series, 104 patients with isolated craniofacial synostosis were retrospectively analyzed. Diagnoses ...
journal_title:Plastic and reconstructive surgery
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doi:10.1097/00006534-199508000-00004
更新日期:1995-08-01 00:00:00
abstract:BACKGROUND:Vascular complications resulting from intravascular filler injection and embolism are major safety concerns for facial filler injection. It is essential to systematically screen full-face arterial variations and help design evidence-based safe filler injection protocols. METHODS:The carotid arteries of 22 c...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0000000000004834
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abstract::The purpose of this article is to present the authors' experience with the use of porous polyethylene ultrathin sheets for orbital floor reconstruction. Thirty-two patients with orbital floor fractures were treated with porous polyethylene ultrathin sheets. Sixteen cases corresponded to orbitozygomatic fractures, 11 c...
journal_title:Plastic and reconstructive surgery
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doi:10.1097/00006534-200203000-00007
更新日期:2002-03-01 00:00:00
abstract::Posttraumatic enophthalmos, though a difficult problem, is correctable by traditional craniofacial techniques. Based on considerable experience with these wide exposure operations, our surgical strategy has evolved and has been distilled into one of limited exposure via lateral upper blepharoplasty, transconjunctival ...
journal_title:Plastic and reconstructive surgery
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更新日期:1998-04-01 00:00:00
abstract:LEARNING OBJECTIVES:After studying this article, the participant should be able to: 1. Identify the subtypes of squamous cell carcinoma of the skin. 2. Identify factors that affect recurrence and/or metastasis. 3. Develop a surgical management plan for treating high-risk squamous cell carcinoma of the skin. In treating...
journal_title:Plastic and reconstructive surgery
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更新日期:2004-11-01 00:00:00
abstract:BACKGROUND:Carpal tunnel syndrome is very common. Although surgery is effective in mild and moderate cases, recovery is often incomplete in severe cases. Therefore, adjuvant therapy to improve nerve regeneration in those patients is much needed. Acetyl-L-carnitine has been shown to be effective in other neuropathies. T...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,随机对照试验
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abstract::Maternal cigarette smoking during pregnancy as a risk factor for having a child with cleft lip/palate has been suggested by several epidemiologic studies. However, most of these studies contained small sample sizes, and a clear association between these two factors could not be established. The U.S. Natality database ...
journal_title:Plastic and reconstructive surgery
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journal_title:Plastic and reconstructive surgery
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journal_title:Plastic and reconstructive surgery
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abstract::Facial nerve injury is one of the most feared complications in surgical rejuvenation of the aging face. Understanding the three-dimensional architecture of the facial soft tissue, the fascial planes that exist within this architectural arrangement, and the danger zones where the facial nerve is situated superficial an...
journal_title:Plastic and reconstructive surgery
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abstract::Seventy-four patients were referred to the senior author because of presumed velopharyngeal incompetence without cleft palate. As a result of an extensive preoperative evaluation that included assessment of articulation patterns, nasal emission, oronasal resonance, and general speech intelligibility, 57 of the 74 pati...
journal_title:Plastic and reconstructive surgery
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更新日期:1984-02-01 00:00:00
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journal_title:Plastic and reconstructive surgery
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doi:10.1097/01.prs.0000436843.15494.ad
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND:Internationally, plastic surgery societies have placed an increasing emphasis on the importance of evidence-based medicine. The authors aimed to categorize levels of evidence of podium presentations at three major North American plastic surgical meetings, and to assess the factors associated with a higher le...
journal_title:Plastic and reconstructive surgery
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doi:10.1097/PRS.0b013e3182818bc3
更新日期:2013-04-01 00:00:00
abstract::Shave excision is a useful technique for the treatment of superficial solar lesions. The most common of these is the solar keratosis, which can be a red or gray scaling lesion. This can at times be difficult to differentiate from the superficial basal cell carcinoma or squamous cell carcinoma or their more infiltratin...
journal_title:Plastic and reconstructive surgery
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doi:10.1097/00006534-198707000-00007
更新日期:1987-07-01 00:00:00
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journal_title:Plastic and reconstructive surgery
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doi:10.1097/PRS.0b013e3181845951
更新日期:2008-10-01 00:00:00
abstract:BACKGROUND:Unplanned surgical readmissions represent a benchmark outcome and pose a considerable cost burden for health care systems. The authors evaluated risk factors associated with readmission following pediatric plastic surgery using a prospective, validated, national database. METHODS:Patients younger than 18 ye...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,评审
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更新日期:2015-02-01 00:00:00
abstract:BACKGROUND:Cranial particulate bone graft heals inlay calvarial defects and can be harvested as early as infancy. The purpose of this study was to test the hypothesis that particulate bone promotes ossification primarily by osteogenesis. METHODS:Freshly harvested particulate bone, devitalized particulate bone, and hig...
journal_title:Plastic and reconstructive surgery
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doi:10.1097/PRS.0b013e31824a2bdd
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abstract:BACKGROUND:Despite the prevalent use of hyaluronic acid-based filling materials for facial soft-tissue augmentation and favorable reports of durability in the infraorbital region, no quantitative data exist on the long-term durability of these products following injection. This study represents the first attempt to use...
journal_title:Plastic and reconstructive surgery
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更新日期:2010-05-01 00:00:00
abstract::Controversy exists in the literature regarding reconstruction of the radial artery after elevation of the radial forearm flap. The literature suggests that reconstructing the radial artery with a vein graft is an important aspect in the use of this flap. In our experience, this has never been performed with no sequela...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::We present our experimental and clinical experiences with the free neurovascular forearm flap. The flap is based on the radial artery, one of the great veins of the forearm (cephalic, basilic, or interconnecting vein), and one or two cutaneous forearm nerves (ulnar, median, or lateral). Because of the standard anatomy...
journal_title:Plastic and reconstructive surgery
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journal_title:Plastic and reconstructive surgery
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更新日期:2011-07-01 00:00:00
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journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
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更新日期:1999-03-01 00:00:00
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journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
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journal_title:Plastic and reconstructive surgery
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更新日期:2015-09-01 00:00:00
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journal_title:Plastic and reconstructive surgery
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更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:One of the goals of lip augmentation is to recreate the natural shape and contour of the lips. The literature describes numerous fillers and multiple techniques for this procedure. However, there is a paucity of information regarding the anatomy of the fat layers of the upper and lower lips. While prosecting...
journal_title:Plastic and reconstructive surgery
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更新日期:2009-07-01 00:00:00
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journal_title:Plastic and reconstructive surgery
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更新日期:2008-10-01 00:00:00
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journal_title:Plastic and reconstructive surgery
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更新日期:1989-10-01 00:00:00
abstract:BACKGROUND:Theories regarding the cause of craniosynostosis that are more than 15 years old cite the role that tensional forces play in the normal and abnormal development of the cranial suture. These theories highlight the effect of stress bands originating from the skull base to the vertex, guiding sutural developmen...
journal_title:Plastic and reconstructive surgery
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