Abstract：
OBJECTIVES:The specific goal of this study was to establish a simple histochemical technique by which the glycosaminoglycan (GAG) lining in the rabbit bladder can be routinely identified. METHODS:Rabbit bladder tissues were fixed in zinc formal, 10% neutral buffered formalin (NBF), 20% NBF, 40% NBF, 2% calcium acetate in 10% NBF, 2% sodium acetate in 10% NBF, 1% cetylpyridinium chloride in 10% NBF, 80% alcohol, histochoice, and 2.5% glutaraldehyde in 0.1 M cacodylate buffer. The following histochemical staining was used: mucicarmine/metanil yellow, colloidal iron, deamination followed by colloidal iron, periodic acid-Schiff, saponification followed by colloidal iron, Alcian blue (AB) at variable pH values, combined aldehyde-fuchsin/AB, performic acid/AB, AB/Alcian yellow, high temperature (60 degrees C) methylation/saponification/AB, and AB/nuclear fast red at pH 5.8 with critical electrolyte concentrations with or without deamination. RESULTS:AB with sodium acetate buffer at pH 5.8 containing 0.6 M or 0.8 M magnesium chloride (MgCl2) showed a well-defined thin GAG lining on the surface of the urothelium, whereas histochemical staining used by previous investigators showed only patchy distribution. There was no observable difference due to the gender, fixative, or region of the bladder from which the tissue was obtained. CONCLUSIONS:A very thin lining of GAG exists in the rabbit bladder which can be localized by AB in sodium acetate buffer at pH 5.8 containing 0.6 M or 0.8 M MgCl2 but not by conventional histochemical techniques. This method now can be applied to answer many questions regarding urothelial function.
journal_name
Urologyjournal_title
Urologyauthors
Grist M,Chakraborty Jdoi
10.1016/s0090-4295(94)80005-7subject
Has Abstractpub_date
1994-07-01 00:00:00pages
26-33issue
1eissn
0090-4295issn
1527-9995pii
S0090-4295(94)80005-7journal_volume
44pub_type
杂志文章,评审相关文献
UROLOGY文献大全abstract：OBJECTIVE:To evaluate whether there is a correlation between publicized health ranking systems and surgical outcomes after radical cystectomy (RC) in New York State (NYS). MATERIALS AND METHODS:Using the Statewide Planning and Research Cooperative System, data were collected in an aggregated fashion per hospital for t...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2015.07.049
更新日期：2015-12-01 00:00:00
abstract：:Aztreonam, the first monobactam antibiotic, represents a significant evolutionary advance in antimicrobial therapy. Aztreonam is stable in the presence of the hydrolytic beta-lactamase enzymes; as such, it is effective against most Gram-negative aerobes, including Pseudomonas aeruginosa, Escherichia coli, Klebsiella, ...
journal_title：Urology
pub_type： 杂志文章,评审
doi：
更新日期：1988-06-01 00:00:00
abstract：:Herein is presented what we believe to be the eighth case reported of renal cell carcinoma with caval tumor thrombus treated by radical excision with vena caval resection. A review of the literature reveals that although postoperative morbidity is minimal, only 1 patient survived more than two years. All patients with...
journal_title：Urology
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：OBJECTIVE:To compare the risk of mortality among men treated for benign prostatic hyperplasia (BPH) with 5 alpha-reductase inhibitors (5ARI) to those treated with alpha-blockers (AB) in community practice settings. METHODS:We employed a retrospective matched cohort study in 4 regions of an integrated healthcare system...
journal_title：Urology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.urology.2018.05.033
更新日期：2018-09-01 00:00:00
abstract：:Ureterocystoplasty enables the bladder to be reliably enlarged and the resultant neobladder to be lined by urothelium. Many techniques have been described for the operative management of the lower ureter, and a number of alternatives have been suggested for reconstruction of the upper renal tract and bladder. This rep...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(98)00363-x
更新日期：1999-03-01 00:00:00
abstract：:We report the case of a calcified angiomyolipoma of the kidney, fortuitously discovered on a computed tomography scan of the abdomen. Because of the presence of intratumoral calcification and macroscopic fat, renal cell carcinoma could not be ruled out by imaging. Biopsy of the mass was performed, and pathological stu...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2016.07.011
更新日期：2016-11-01 00:00:00
abstract：:Turner syndrome is a chromosomal disorder that occurs in an estimated 1 in 2500 female live births. It is estimated that 6%-12% of all Turner syndrome patients will be a mosaic with Y-chromosomal elements putting them at risk for gonadoblastoma and subsequent dysgerminoma. While 30%-50% of this population demonstrate ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2020.01.031
更新日期：2020-05-01 00:00:00
abstract：:Placenta percreta is a rare complication of pregnancy in which the chorionic villi penetrate through the myometrium causing uterine rupture and life-threatening hemorrhage. Bladder invasion by the villi is unusual and may be associated with hematuria and low abdominal pain during midterm pregnancy. ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(92)90216-j
更新日期：1992-04-01 00:00:00
abstract：:We reviewed the clinical course and pathologic findings of 17 patients with adenocarcinoma of the urinary bladder at Massachusetts General Hospital between 1962 and 1978. The 12 men and 5 women were between thirty-eight and eighty-six years old (mean, sixty years). Five patients had urachal adenocarcinoma, 8 had pure ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(83)90117-6
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVES:To determine the best preventive strategies for bladder calculi in children with an augmented bladder, the risk factors and prevention strategies for urolithiasis were evaluated. METHODS:The records of 89 patients following augmentation cystoplasty were reviewed to assess the results of augmentation cystopl...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(00)00663-4
更新日期：2000-09-01 00:00:00
abstract：OBJECTIVE:To evaluate sexual dysfunction and enlargement of seminal vesicles in sexually active men who were treated by α1-blockers for benign prostatic hyperplasia and its possible clinical application. MATERIALS AND METHODS:This is a prospective cohort study from January 2015 to December 2016. We enrolled sexually a...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2017.08.025
更新日期：2018-08-01 00:00:00
abstract：OBJECTIVE:To study the relation between uroflow Stop Test and early recovery of potency following robot-assisted radical prostatectomy (RARP). We recently showed that the ability to completely stop urine flow during voiding, measured objectively by uroflowmetry at the time of catheter removal (uroflow Stop Test) can pr...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2015.05.041
更新日期：2015-10-01 00:00:00
abstract：:Transrectal ultrasound may establish the diagnosis of prostatic abscess in an ambiguous clinical setting. Transurethral resection (deroofing) is the treatment preferred by many clinicians, yet intraoperative complete abscess obliteration may be difficult to confirm endoscopically. We report on a patient with a complex...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(91)80117-p
更新日期：1991-05-01 00:00:00
abstract：OBJECTIVES:The combined use of total prostate-specific antigen (PSA), clinical stage, and Gleason score accurately predicts final pathologic stage for men with clinically localized prostate cancer. Recently, the free/ total PSA ratio has been proposed as an adjunct for early detection of prostate cancer. We examined th...
journal_title：Urology
pub_type： 临床试验,杂志文章
doi：10.1016/s0090-4295(96)00610-3
更新日期：1996-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the correlation between Visual Prostate Symptom Score (VPSS) and the International Prostate Symptom Score (IPSS) and uroflowmetry parameters in men with lower urinary tract symptoms (LUTS) with literature review. METHODS:Patients were pooled from 4 different urology clinics in Turkey and divided ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2020.09.001
更新日期：2020-12-01 00:00:00
abstract：:The compartment syndrome is a rare complication of surgery in the lithotomy position, but one that may result in neurovascular damage and permanent disability. Herein, we present a case of bilateral three-compartment syndrome following urethroplasty in the dorsolithotomy position, with review of the literature and dis...
journal_title：Urology
pub_type： 杂志文章,评审
doi：10.1016/0090-4295(94)90204-6
更新日期：1994-05-01 00:00:00
abstract：:An eighty-eight-year-old Puerto Rican presented with painless enlargment of one testicle which proved histologically to be due to Schistosoma mansoni. Intratesticular ovum deposits were not associated with inflammation, granulomas, or infarction, but marked sclerosing periorchitis was present. The case illustrates a v...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(78)90378-3
更新日期：1978-07-01 00:00:00
abstract：OBJECTIVES:To share our experience with nondismembered ureteroplasty (NDU), a novel application of an old technique in congenital midureteral stenosis (CMS). METHODS:A 35-year-old man was diagnosed as a case of CMS, a rare benign condition on the basis of extensive evaluation, including ultrasonography, intravenous ur...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2010.03.093
更新日期：2010-10-01 00:00:00
abstract：:A new modification of transvaginal technique of bladder neck suspension is described. Its main merit is its safety. The ligature-carrying needles are inserted from below rather than above. It virtually eliminates bladder injury. Our experience with 29 cases and a follow-up of six to thirty months shows that this techn...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(90)80258-o
更新日期：1990-09-01 00:00:00
abstract：:The effects of serotonergic drugs on the central nervous system control of the lower urinary tract have revealed possible approaches for the treatment of detrusor overactivity and urinary incontinence. Studies in animals of the distribution of serotonergic nerves and receptors and the changes in voiding function induc...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(01)01636-3
更新日期：2002-05-01 00:00:00
abstract：OBJECTIVES:To determine whether an association exists between C-reactive protein (CRP) levels and lower urinary tract symptoms (LUTS) as assessed by the American Urological Association Symptom Index (AUA-SI) among both men and women, and to determine the association of CRP levels with the individual urologic symptoms c...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2008.12.012
更新日期：2009-05-01 00:00:00
abstract：:Discussion, diagnosis, and management of fibroepithelial polyps in a newborn female. ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2019.06.026
更新日期：2019-10-01 00:00:00
abstract：OBJECTIVES:To investigate the clinical utility of the subforms of free prostate-specific antigen (PSA), namely proPSA and "benign" PSA (BPSA), to improve cancer detection when the percent free PSA level is less than 15%. Percent free PSA, while maintaining sensitivity, has greatly improved the specificity of PSA for th...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2004.06.033
更新日期：2004-12-01 00:00:00
abstract：OBJECTIVES:To better understand the outcomes and management of patients when there is a failure to visualize kidneys on prenatal ultrasound. METHODS:Nine thousand five hundred twelve prenatal ultrasound studies performed on 4900 patients were reviewed retrospectively for the findings of a failure to visualize kidneys....
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(98)00190-3
更新日期：1998-08-01 00:00:00
abstract：OBJECTIVES:To evaluate the use of laparoscopic techniques in patients with a renal transplant. METHODS:Since 1992, 358 patients have undergone urologic laparoscopy at our hospital. Among these, 37 procedures (10.4%) were performed in patients with a renal transplant: 14 bilateral nephrectomies for severe drug-resistan...
journal_title：Urology
pub_type： 临床试验,杂志文章
doi：10.1016/s0090-4295(96)00559-6
更新日期：1997-04-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of low-dose human chorionic gonadotropin (HCG) administration on structural changes in the lower urinary tract in boys with urinary incontinence in the setting of bladder exstrophy-epispadias complex (BEEC). PATIENTS AND METHODS:We prospectively evaluated 30 patients (median age 7.5...
journal_title：Urology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.urology.2016.12.034
更新日期：2017-03-01 00:00:00
abstract：:Urinary calcium excretion was measured in 100 consecutive normocalcemic patients with calcium urolithiasis and 12 Saudi Arabian controls while the patients and the controls were eating their usual diet of unknown calcium content. Only 16 patients were hypercalciuric using the definition of twenty-four-hour urinary cal...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(85)90249-3
更新日期：1985-07-01 00:00:00
abstract：OBJECTIVE:To investigate the potential protective effects of selenium and lycopene, either alone or in combination, for cisplatin-induced oxidative stress and testicular dysfunction in male rats. METHODS:A total of 50 adult male Wistar rats were divided into 5 groups of 10 animals each, as follows: control group (trea...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2011.12.006
更新日期：2012-05-01 00:00:00
abstract：OBJECTIVE:To report the long-term follow-up results of patients with Hinman-Allen syndrome (HAS) at our institution. METHODS:The data from 22 children with HAS were retrospectively analyzed. The patients were followed up every 3-6 months with serial physical examinations, voiding charts, urine culture, postvoid residu...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2011.08.018
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVES:To characterize ultrasound bladder measures, and to determine whether these measures were associated with measures of lower urinary tract dysfunction. METHODS:Three-dimensional ultrasounds were used to assess bladder surface area (SA), bladder wall thickness (BWT), and estimated bladder weight (EBW) in a ra...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2009.04.081
更新日期：2009-10-01 00:00:00
