Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets.

abstract：:The 60S ribosomal subunits from Saccharomyces cerevisiae contain a set of four acidic proteins named YP1alpha, YP1beta, YP2alpha, and YP2beta. The genes for each were PCR amplified from a yeast cDNA library, sequenced, and expressed in Escherichia coli cells using two expression systems. The first system, pLM1, was us...

journal_title：Protein expression and purification

authors： Tchórzewski M,Boguszewska A,Abramczyk D,Grankowski N

更新日期：1999-02-01 00:00:00

abstract：:A T7 promoter-based His6-tagging vector has been constructed that directs the synthesis in Escherichia coli of fusion proteins containing a stretch of six histidine residues at the N terminus. The vector allows overproduction and single-step purification of His6-fusion protein by immobilized metal (Ni2+) chelate affin...

journal_title：Protein expression and purification

authors： Chen J,Li Y,Wang E,Wang Y

更新日期：1999-02-01 00:00:00

abstract：:We have constructed and optimized a high yielding Escherichia coli expression system to produce glycosylation-free human procathepsin K and have developed conditions for refolding this enzyme. Recombinant human procathepsin K (EC 3.4.22.38) was expressed in E. coli, refolded from inclusion bodies, and further purified...

journal_title：Protein expression and purification

authors： D'alessio KJ,McQueney MS,Brun KA,Orsini MJ,Debouck CM

更新日期：1999-03-01 00:00:00

abstract：:The heat-stable protease from Chryseobacterium indologenes Ix9a was purified to homogeneity using immobilized metal affinity chromatography. The enzyme was characterized as a metalloprotease with an approximate relative molecular mass of 24,000, a pH optimum of 6.5, and a high temperature optimum (50 degrees C). The m...

journal_title：Protein expression and purification

authors： Venter H,Osthoff G,Litthauer D

更新日期：1999-04-01 00:00:00

abstract：:ATP sulfurylase cDNA from MET3 on chromosome X of Saccharomyces cerevisiae was amplified and cloned, and recombinant ATP sulfurylase was expressed in Escherichia coli. The synthesis of ATP sulfurylase was directed by an expression system that employs the regulatory genes of the luminous bacterium Vibrio fischeri. A so...

journal_title：Protein expression and purification

authors： Karamohamed S,Nilsson J,Nourizad K,Ronaghi M,Pettersson B,Nyrén P

更新日期：1999-04-01 00:00:00

abstract：:We have developed a baculovirus expression system for the rapid and efficient production of large quantities (>5 mg/10(8) cells) of ICP8. The recombinant ICP8 is fully functional and binds to single-stranded DNA. Secondary structure calculations from circular dichroism measurements indicate a content of 34.5% alpha-he...

journal_title：Protein expression and purification

authors： Spatz M,Ali SA,Auer M,Graf C,Eibl MM,Steinkasserer A

更新日期：1999-06-01 00:00:00

abstract：:An increasing demand for isotopically labeled samples for spectroscopic and crystallographic studies has led to a corresponding need for effective and efficient methods for producing these samples. The present work is based on the strategy of using an isotopically labeled compound as the growth-limiting nutrient durin...

journal_title：Protein expression and purification

authors： Studts JM,Fox BG

更新日期：1999-06-01 00:00:00

abstract：:Insulin-like growth factors (IGFs) in the circulation are predominantly sequestered into ternary complexes comprising IGF, IGF-binding protein-3 (IGFBP-3), and the acid-labile subunit (ALS). Besides its role in regulating IGF bioavailability in the circulation, IGFBP-3 has both IGF-dependent and IGF-independent action...

journal_title：Protein expression and purification

authors： Firth SM,Ganeshprasad U,Poronnik P,Cook DI,Baxter RC

更新日期：1999-06-01 00:00:00

abstract：:Apolipoprotein (apo) E plays an important role in lipid metabolism, and the major isoforms of apoE (apoE2, apoE3, and apoE4) have significantly different metabolic effects. Apolipoprotein E4 is associated with a higher risk of both heart disease and Alzheimer's disease (AD). Patients homozygous for apolipoprotein E2 a...

journal_title：Protein expression and purification

authors： Morrow JA,Arnold KS,Weisgraber KH

更新日期：1999-07-01 00:00:00

abstract：:Full-length human gelatinase B (FLGelB) and its C-terminal truncated form (dGelB) were expressed in Pichia pastoris strain GS115, using the Saccharomyces cerevisiae Mat alpha signal peptide. In both cases, a high level of the secreted protein could be detected by SDS-PAGE. The truncated gene was also expressed using t...

journal_title：Protein expression and purification

authors： Roy N,Padmanabhan S,Smith M,Shi L,Navre M,Das G

更新日期：1999-07-01 00:00:00

abstract：:AX2 is a 46-amino-acid cysteine-rich peptide isolated from sugar beet leaves infected with the fungus Cercospora beticola (Sacc.). AX2 strongly inhibits the growth of C. beticola and other filamentous fungi, but has little or no effect against bacteria. AX2 is produced in very low amounts in sugar beet leaves, and to ...

journal_title：Protein expression and purification

authors： Kristensen AK,Brunstedt J,Nielsen JE,Mikkelsen JD,Roepstorff P,Nielsen KK

更新日期：1999-08-01 00:00:00

abstract：:Transition protein 2 (TP2; 137 amino acid residues) from boar late spermatid nuclei has three potential zinc finger motifs in the N-terminal 34 region. Gel shift assays revealed that boar TP2 recognized a CpG island sequence in a zinc-dependent manner. However, there was some nonspecific recognition of the oligonucleo...

journal_title：Protein expression and purification

authors： Sato H,Akama K,Kojima S,Miura K,Sekine A,Nakano M

更新日期：1999-08-01 00:00:00

abstract：:Fibrillarin is a 34-kDa nucleolar protein associated with many of the small nucleolar ribonucleoprotein (snoRNP) particles and plays a role in ribosomal RNA processing. A subset of patients with the systemic autoimmune disease Scleroderma produce autoantibodies against fibrillarin and it is a genetically restricted ta...

journal_title：Protein expression and purification

authors： Pearson DL,Reimonenq RD,Pollard KM

更新日期：1999-10-01 00:00:00

abstract：:The first amino acid of "authentic" poliovirus RNA-dependent RNA polymerase, 3D(pol), is a glycine. As a result, production of 3D(pol) in Escherichia coli requires addition of an initiation codon; thus, a formylmethionine is added to the amino terminus. The formylmethionine should be removed by the combined action of ...

journal_title：Protein expression and purification

authors： Gohara DW,Ha CS,Kumar S,Ghosh B,Arnold JJ,Wisniewski TJ,Cameron CE

更新日期：1999-10-01 00:00:00

abstract：:Labeled proteins are used in a variety of applications. This review focuses on methods that utilize genetic engineering to introduce protein kinase recognition sites into proteins. Many protein kinase recognition sites can be introduced into proteins and serve as useful tags for a variety of purposes. The introduction...

journal_title：Protein expression and purification

authors： Pestka S,Lin L,Wu W,Izotova L

更新日期：1999-11-01 00:00:00

abstract：:Antibodies to DNA define an important autospecificity that arises in systemic lupus erythematosus (SLE). To elucidate the molecular features that may explain the pathogenesis of SLE, a heterologous system for expression of cloned V genes is often desirable. Here, a single-chain Fv coding domain was constructed by usin...

journal_title：Protein expression and purification

authors： Cocca BA,Seal SN,Radic MZ

更新日期：1999-11-01 00:00:00

abstract：:Haemophilus influenzae is a common inhabitant of the upper respiratory tract and can cause serious infections of mucosal surfaces. Results from recent studies indicate that this pathogen possesses copious amounts of surface-localized phosphomonoesterase activity mediated by the bacterial lipoprotein e (P4). While the ...

journal_title：Protein expression and purification

authors： Reilly TJ,Smith AL

更新日期：1999-12-01 00:00:00

abstract：:Recombinant major capsid protein, L1 (M(r) = 55,000), of human papillomavirus type 11 was expressed intracellularly at high levels in a galactose-inducible Saccharomyces cerevisiae expression system by an HPV6/11 hybrid gene. The capsid protein self-assembled into virus-like particles (VLPs) and accounted for 15% of t...

journal_title：Protein expression and purification

authors： Cook JC,Joyce JG,George HA,Schultz LD,Hurni WM,Jansen KU,Hepler RW,Ip C,Lowe RS,Keller PM,Lehman ED

更新日期：1999-12-01 00:00:00

abstract：:Micrococcus luteus isolated from human skin secretes an alkaline protease which degrades elastin. M. luteus protease (MLP) was produced in the late logarithmic and stationary phases of growth. MLP, purified to homogeneity by a three-step process, had a molecular mass of 32,812 Da and an isoelectric point of 9.3. MLP w...

journal_title：Protein expression and purification

authors： Clark DJ,Hawrylik SJ,Kavanagh E,Opheim DJ

更新日期：2000-02-01 00:00:00

abstract：:HPLC6 is the major component of liver-type antifreeze polypeptides (AFPs) from the winter flounder, Pleuronectes americanus. To facilitate mutagenesis studies of this protein, a gene encoding the 37-amino acid mature polypeptide was chemically synthesized and cloned into the Tac cassette immediately after the bacteria...

journal_title：Protein expression and purification

authors： Tong L,Lin Q,Wong WK,Ali A,Lim D,Sung WL,Hew CL,Yang DS

更新日期：2000-03-01 00:00:00

abstract：:This paper describes the overexpression of the Rgp-1 (arginine) protease domain from Porphyromonas gingivalis. This protease and the related Kgp (lysine) protease, both of which display trypsin-like specificity, have been implicated as major virulence factors and may play a significant role in the etiology of periodon...

journal_title：Protein expression and purification

authors： Margetts MB,Barr IG,Webb EA

更新日期：2000-04-01 00:00:00

abstract：:Recombinant varicella zoster virus (VZV) thymidine kinase (TK) was isolated in a fast and gentle two-step procedure from Escherichia coli. The TK was expressed as a PreScission-cleavable fusion protein and purified by glutathione and ATP affinity chromatography, yielding homogeneous, highly pure VZV TK. The purified e...

journal_title：Protein expression and purification

authors： Amrhein I,Wurth C,Bohner T,Hofbauer R,Folkers G,Scapozza L

更新日期：2000-04-01 00:00:00

abstract：:Snake venoms are rich sources of proteases that strongly affect the vascular system, by promoting blood coagulation, hemorrhage, and fibrinolysis. Hemorrhagic activity is mostly due to the enzymatic action of metalloproteases on capillary basement membrane components, such as collagen IV, laminin, and fibronectin. A f...

journal_title：Protein expression and purification

authors： Selistre-de-Araujo HS,de Souza EL,Beltramini LM,Ownby CL,Souza DH

更新日期：2000-06-01 00:00:00

abstract：:SPC1 (furin/PACE), an enzyme belonging to the S8 group of serine endoproteases, is a type I integral membrane protein that catalyzes the processing of a multitude of precursor proteins. We report here the use of transfected Drosophila melanogaster Schneider 2 cells to produce milligram amounts of two forms of recombin...

journal_title：Protein expression and purification

authors： Denault JB,Lazure C,Day R,Leduc R

更新日期：2000-06-01 00:00:00

abstract：:alpha-N-Acetylglucosaminidase (EC 3.2.1.50) is a lysosomal enzyme that is deficient in the genetic disorder Sanfilippo syndrome type B. To study the human enzyme, we expressed its cDNA in Lec1 mutant Chinese hamster ovary (CHO) cells, which do not synthesize complex oligosaccharides. The enzyme was purified to apparen...

journal_title：Protein expression and purification

authors： Zhao KW,Neufeld EF

更新日期：2000-06-01 00:00:00

abstract：:A vector was constructed for intracellular expression of the Arabidopsis thaliana DnaJ homologue AtJ2 in the methylotrophic yeast Pichia pastoris. The vector includes DNA encoding an amino-terminal histidine-tag, to simplify protein purification. Shake-flask cultures could be induced to produce approximately 250 mg/ L...

journal_title：Protein expression and purification

authors： Zhou R,Kroczyńska B,Miernyk JA

更新日期：2000-07-01 00:00:00

abstract：:A 5'-truncated PDE4A-cDNA corresponding to the amino acid positions 200-886 of the "full-length" sequence (Accession No. L20965) was generated from human leukocyte mRNA by RT-PCR. Several PDE4A constructs containing the catalytic region and differing in their degree of N- and/or C-terminal truncation (amino acid posit...

journal_title：Protein expression and purification

authors： Richter W,Hermsdorf T,Lilie H,Egerland U,Rudolph R,Kronbach T,Dettmer D

更新日期：2000-08-01 00:00:00

abstract：:Individual, soluble human alpha-globin chains were expressed in bacteria with exogenous heme and methionine aminopeptidase. The yields of soluble alpha chains in bacteria were comparable to those of recombinant non-alpha chains expressed under the same conditions. Molecular mass and gel-filtration properties of purifi...

journal_title：Protein expression and purification

authors： Adachi K,Yamaguchi T,Yang Y,Konitzer PT,Pang J,Reddy KS,Ivanova M,Ferrone F,Surrey S

更新日期：2000-10-01 00:00:00

abstract：:Galactose oxidase catalyzes the oxidation of a variety of primary alcohols, producing hydrogen peroxide as a product. Among hexose sugars, the enzyme exhibits a high degree of specificity for the C6-hydroxyl of galactose and its derivatives, underlying a number of important bioanalytical applications. Galactose oxidas...

journal_title：Protein expression and purification

authors： Whittaker MM,Whittaker JW

更新日期：2000-10-01 00:00:00

abstract：:Three thermostable proteases, designated S, N, and B, are extracellular enzymes produced by Bacillus stearothermophilus strain TLS33. They were purified by lysine affinity chromatography, strong anion exchange Q HyperD chromatography, and Ultrogel AcA44 gel filtration. The molecular masses of the enzymes determined by...

journal_title：Protein expression and purification

authors： Sookkheo B,Sinchaikul S,Phutrakul S,Chen ST

更新日期：2000-11-01 00:00:00