Abstract:
AIMS:Although excessive fat and caffeine intake are independent risk factors for bone microstructural and functional disturbances, their association remains overlooked. Thus, we investigated the impact of high-fat diet (HFD) and caffeine alone and combined on serum lipid profile, bone microstructure, micromineral distribution and biomechanical properties. METHODS:Forty female C57BL/6 mice were randomized into 4 groups daily treated for seventeen weeks with standard diet (SD) or HFD (cafeteria diet) alone or combined with 50 mg/kg caffeine. KEY FINDINGS:The association between HFD and caffeine reduced the weight gain compared to animals receiving HFD alone. Caffeine alone or combined with HFD increases total and HDL cholesterol circulating levels. HFD also reduced calcium, phosphorus and magnesium bone levels compared to the groups receiving SD, and this reduction was aggravated by caffeine coadministration. From biomechanical assays, HFD combined with caffeine increased bending strength and stiffness of tibia, a finding aligned with the marked microstructural remodeling of the cortical and cancellous bone in animals receiving this combination. SIGNIFICANCE:Our findings indicated that HFD and caffeine interact to induce metabolic changes and bone microstructural remodeling, which are potentially related to bone biomechanical adaptations in response to HFD and caffeine coadministration.
journal_name
Life Scijournal_title
Life sciencesauthors
de Souza FB,Novaes RD,Santos CFF,de Deus FA,Santos FC,Ladeira LCM,Gonçalves RV,Bastos DSS,Souza ACF,Machado-Neves M,Dos Santos ECdoi
10.1016/j.lfs.2021.119450keywords:
["Bone health","Bone morphology","Cafeteria diet","Caffeine","Tissue remodeling"]subject
Has Abstractpub_date
2021-07-01 00:00:00pages
119450eissn
0024-3205issn
1879-0631pii
S0024-3205(21)00435-5journal_volume
276pub_type
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