Abstract：
:Herein, we report a method that combined "aptamer-locker" DNA with CRISPR/Cas12a-based biosensing for sensitive and rapid melamine analysis. Three strategies were harnessed for designing the DNA sensors that were well characterized by circular dichroism (CD) spectroscopy and isothermal titration calorimetry (ITC) in the absence and presence of melamine. The detection parameters were optimized to achieve good analytic performance. As a result, a limit of detection (LOD) as low as 38 nM was achieved, which is below the threshold (1.0 mg/kg) of allowable melamine in infant milk products. In addition, the sensors show high selectivity for melamine against other analogues such as cyanuric acid, ammeline and ammelide. Moreover, our method was effective for rapid melamine analysis in whole milk samples, with or without sample pretreatment, in less than 20 min. Adopting a commercially available portable fluorimeter, on-site analysis of melamine in milk was accomplished. The strategies demonstrated here can expand to detect other non-nucleic-acid targets by simply replacing the aptamers.
journal_name
Biosens Bioelectronjournal_title
Biosensors & bioelectronicsauthors
Qiao B,Xu J,Yin W,Xin W,Ma L,Qiao J,Liu Ydoi
10.1016/j.bios.2021.113233keywords:
["Aptamer","Biosensor","CRISPR\/Cas12a","Melamine","On-site test"]subject
Has Abstractpub_date
2021-07-01 00:00:00pages
113233eissn
0956-5663issn
1873-4235pii
S0956-5663(21)00270-0journal_volume
183pub_type
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