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Aflatoxigenic fungi and aflatoxin in cocoa.

Abstract：

:This paper reports the occurrence of aflatoxigenic fungi and the presence of aflatoxins in 226 cocoa samples collected on Brazilian farms. The samples were taken at various stages of fermentation, drying and storage. A total of 819 potentially aflatoxigenic fungi were isolated using Dichloran 18% Glycerol agar after surface disinfection, and identified by standard techniques. The ability of the fungi to produce aflatoxins was determined using the agar plug technique and TLC. The presence of aflatoxins in cocoa samples was determined by HPLC using post-column derivatization with bromide after immunoaffinity column clean up. The aflatoxigenic fungi isolated were Aspergillus flavus, A. parasiticus and A. nomius. A considerable increase in numbers of these species was observed during drying and storage. In spite of the high prevalence of aflatoxigenic fungi, only low levels of aflatoxin were found in the cocoa samples, suggesting the existence of limiting factors to the accumulation of aflatoxins in the beans.

journal_name

Int J Food Microbiol

journal_title

International journal of food microbiology

authors

Copetti MV,Iamanaka BT,Pereira JL,Fungaro MH,Taniwaki MH

doi

10.1016/j.ijfoodmicro.2011.05.020

subject

Has Abstract

pub_date

2011-08-02 00:00:00

pages

141-4

issue

2

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(11)00303-5

journal_volume

148

pub_type

杂志文章

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