Abstract:
:In this study, we describe a simple system in which human keratinocytes can be redirected to an alternative differentiation pathway. We transiently transfected freshly isolated human skin keratinocytes with the single transcription factor OCT4. Within 2 days these cells displayed expression of endogenous embryonic genes and showed reduced genomic methylation. More importantly, these cells could be specifically converted into neuronal and contractile mesenchymal cell types. Redirected differentiation was confirmed by expression of neuronal and mesenchymal cell mRNA and protein, and through a functional assay in which the newly differentiated mesenchymal cells contracted collagen gels as efficiently as authentic myofibroblasts. Thus, to generate patient-specific cells for therapeutic purposes, it may not be necessary to completely reprogram somatic cells into induced pluripotent stem cells before altering their differentiation and grafting them into new tissues.
journal_name
Gene Therjournal_title
Gene therapyauthors
Racila D,Winter M,Said M,Tomanek-Chalkley A,Wiechert S,Eckert RL,Bickenbach JRdoi
10.1038/gt.2010.148subject
Has Abstractpub_date
2011-03-01 00:00:00pages
294-303issue
3eissn
0969-7128issn
1476-5462pii
gt2010148journal_volume
18pub_type
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