Abstract：
:The incidence of Salmonella spp. in tropical seafood was studied using standard microbiological techniques and polymerase chain reaction (PCR). Six of 20 finfish (30%), 4 of 20 clams (20%) and 1 of 20 shrimp (5%) were positive by culture techniques and by PCR. In a comparative study of different selective enrichment broths and selective plating media, more than one enrichment broth and selective agar were found to be necessary for efficient detection of Salmonella from seafood. Selenite cystine broth (SCB) was found to be more efficient compared to tetrathionate broth (TTB) while both bismuth sulfite agar (BSA) and hektoen enteric agar (HEA) were equally effective as selective plating media for fish. In the case of clams, HEA was found to be more effective. The presence of Salmonella spp. could be detected by PCR amplification of DNA extracted directly from the enrichment broths. In two cases, enrichment broths that were positive by PCR did not yield Salmonella by conventional methods.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Sanath Kumar H,Sunil R,Venugopal MN,Karunasagar I,Karunasagar Idoi
10.1016/s0168-1605(03)00144-2subject
Has Abstractpub_date
2003-11-15 00:00:00pages
91-5issue
1eissn
0168-1605issn
1879-3460pii
S0168160503001442journal_volume
88pub_type
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journal_title：International journal of food microbiology
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