Abstract:
:1. The regulation of adenosine 3':5'-cyclic monophosphate (cyclic AMP) levels by cholinoceptors has been studied in cultured bovine adrenal medullary cells. 2. Acetylcholine (100 microM), nicotine (10 microM) and dimethylphenylpiperazinium (20 microM) each increased cellular cyclic AMP levels 2 to 4 fold over 5 min in the absence of phosphodiesterase inhibitors. The muscarinic agonist acetyl-beta-methylcholine (100 microM) had no effect either on its own or on the response to nicotine. The responses to acetylcholine and nicotine were unaffected by atropine (1 microM) but were abolished by mecamylamine (5 microM). 3. Cellular cyclic AMP increased transiently during continuous exposure to nicotine (1-20 microM), with the largest response seen after 5 min, a smaller response after 20 min, and no change in cyclic AMP levels seen after 90 or 180 min. The maximal response after 5 min stimulation was seen with 5-10 microM nicotine and the EC50 was about 2 microM. In contrast, extracellular cyclic AMP levels did not change after 5 or 20 min stimulation with nicotine, but increased slightly after 90 min and further after 180 min. 4. The cellular cyclic AMP response to nicotine (10 microM) was unchanged or weakly enhanced in the presence of the unselective phosphodiesterase inhibitor, isobutylmethylxanthine, and was unchanged in the presence of rolipram. Nicotine did not interact synergistically with low concentrations of forskolin. The response was however completely abolished in the absence of extracellular Ca2+.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Anderson K,Robinson PJ,Marley PDdoi
10.1111/j.1476-5381.1992.tb14341.xsubject
Has Abstractpub_date
1992-06-01 00:00:00pages
360-6issue
2eissn
0007-1188issn
1476-5381journal_volume
106pub_type
杂志文章abstract::If one drug, B, antagonizes another, A, by producing the opposite physiological effect, the antagonist concentration-effect curves should be affected by the recording system, which limits the range of agonist responses. With pieces of isolated guinea-pig ileum taken from adjacent parts of the same animal, one recorded...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1038/sj.bjp.0704169
更新日期:2001-08-01 00:00:00
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journal_title:British journal of pharmacology
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更新日期:1983-10-01 00:00:00
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journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1970.tb09553.x
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journal_title:British journal of pharmacology
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journal_title:British journal of pharmacology
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journal_title:British journal of pharmacology
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journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1985.tb16144.x
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更新日期:1995-09-01 00:00:00
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journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1978.tb07791.x
更新日期:1978-05-01 00:00:00
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更新日期:1998-07-01 00:00:00
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更新日期:2009-01-01 00:00:00
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更新日期:1987-10-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:1997-12-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/bph.13413
更新日期:2016-03-01 00:00:00
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更新日期:2005-08-01 00:00:00