Modification of intracellular free calcium in cultured A10 vascular smooth muscle cells by exogenous phosphatidic acid.


:Exogenous phosphatidic acid (PA) was observed to produce a concentration-dependent increase in [Ca(2+)](i) in cultured A10 vascular smooth muscle cells. Preincubation of cells with sarcoplasmic reticulum Ca(2+)-ATPase inhibitors (cyclopiazonic acid and thapsigargin), a phospholipase C inhibitor (2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate), inositol 1,4,5-trisphosphate receptor antagonists (2-aminoethoxydiphenyl borate and xestospongin), and an activator of protein kinase C (PKC) (phorbol 12-myristate 13-acetate) depressed the PA-evoked increase in [Ca(2+)](i). Although EGTA, an extracellular Ca(2+) chelator, decreased the PA-induced increase in [Ca(2+)](i), sarcolemmal Ca(2+)-channel blockers (verapamil or diltiazem) did not alter the action of PA. On the other hand, inhibitors of PKC (bisindolylmaleimide I) and G(i)-protein (pertussis toxin) potentiated the increase in [Ca(2+)](i) evoked by PA significantly. These results suggest that the PA-induced increase in [Ca(2+)](i) in vascular smooth muscle cells may occur upon the activation of phospholipase C and the subsequent release of Ca(2+) from the inositol 1,4,5-trisphosphate-sensitive Ca(2+) pool in the sarcoplasmic reticulum. This action of PA may be mediated through the involvement of PKC.


Biochem Pharmacol


Biochemical pharmacology


Bhugra P,Xu YJ,Rathi S,Dhalla NS




Has Abstract


2003-06-15 00:00:00














  • Role of cyclic GMP in the mediation of circadian rhythmicity of the adenylate cyclase-cyclic AMP-phosphodiesterase system in Euglena.

    abstract::Cyclic AMP (cAMP) and cyclic GMP (cGMP) are two second messengers that have been proposed to act as a dualistic system in biological regulation. To determine if cGMP plays a role in the mediation of circadian rhythmicity of the adenylate cyclase (AC)-cAMP-phosphodiesterase (PDE) system in the achlorophyllous ZC mutant...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Tong J,Edmunds LN Jr

    更新日期:1993-05-25 00:00:00

  • "Supersulfated" heparin fragments, a new type of low-molecular weight heparin. Physico-chemical and pharmacological properties.

    abstract::A new type of low-molecular-weight heparin (ss-LMW-H) was prepared (by controlled depolymerization and concurrent sulfation of heparin with a mixture of sulfuric and chlorosulfonic acid), to test the influence of extra-sulfate groups on biological properties of heparin fragments. The fragments had an average molecular...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Naggi A,Torri G,Casu B,Pangrazzi J,Abbadini M,Zametta M,Donati MB,Lansen J,Maffrand JP

    更新日期:1987-06-15 00:00:00

  • Substituted flavones as aryl hydrocarbon (Ah) receptor agonists and antagonists.

    abstract::The structure-dependent aryl hydrocarbon (Ah) receptor agonist and antagonist activities of the following substituted flavones were investigated: flavone, 4'-methoxy-, 4'-amino-, 4'-chloro-, 4'-bromo-, 4'-nitro-, 4'-chloro-3'-nitro-, 3'-amino-4'-hydroxy-, 3',4'-dichloro-, and 4'-iodoflavone. The halogenated flavones e...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Lu YF,Santostefano M,Cunningham BD,Threadgill MD,Safe S

    更新日期:1996-04-26 00:00:00

  • New insights into generalized hepatoprotective effects of oleanolic acid: key roles of metallothionein and Nrf2 induction.

    abstract::Oleanolic acid (OA) is a natural triperpenoid that protects against a variety of hepatotoxicants such as carbon tetrachloride, cadmium, acetaminophen, and bromobenzene. To gain insight into the molecular mechanisms of this generalized hepatoprotection, genomic analysis was performed on mouse and rat livers after OA tr...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Liu J,Wu Q,Lu YF,Pi J

    更新日期:2008-10-01 00:00:00

  • A novel action of the antianginal drug bepredil: induction of internal Ca(2+) release and external Ca(2+) influx in Madin-Darby canine kidney (MDCK) epithelial cells.

    abstract::The effect of the antianginal drug bepridil on Ca(2+) signaling in Madin-Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca(2+) probe. Bepridil at 10-50 microM evoked a significant rise in cytosolic free Ca(2+) concentration ([Ca(2+)](i)) in a dose-dependent manner. The [Ca(2+)](i) rise consiste...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Jan CR,Tseng CJ

    更新日期:2000-03-15 00:00:00

  • Effect of human serum albumin on transplacental transfer of glyburide.

    abstract::Glyburide is a second-generation sulfonylurea hypoglycemic drug used for the treatment of select women with pregestational and gestational diabetes mellitus (GDM). In vitro and in vivo investigations demonstrated its very low transplacental transfer to the fetal circulation. However, the factors influencing its low tr...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Nanovskaya TN,Nekhayeva I,Hankins GD,Ahmed MS

    更新日期:2006-08-28 00:00:00

  • Potentiation by febrifugine of host defense in mice against Plasmodium berghei NK65.

    abstract::The effect of febrifugine, the main alkaloidal constituent of an antimalarial crude drug, Dichroa febrifuga Lour., on protective immunity in mice infected with erythrocytic stage Plasmodium berghei NK65 was investigated. Febrifugine was administered orally, at a dose of 1 mg/kg/day, to mice before and/or after they we...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Murata K,Takano F,Fushiya S,Oshima Y

    更新日期:1999-11-15 00:00:00

  • Venom-derived modulators of epilepsy-related ion channels.

    abstract::Epilepsy is characterised by spontaneous recurrent seizures that are caused by an imbalance between neuronal excitability and inhibition. Since ion channels play fundamental roles in the generation and propagation of action potentials as well as neurotransmitter release at a subset of excitatory and inhibitory synapse...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Chow CY,Absalom N,Biggs K,King GF,Ma L

    更新日期:2020-11-01 00:00:00

  • Inhibition of rat brain pyruvate dehydrogenase by thiamine analogs.

    abstract::The effects of thiamine thiazolone (TT) and thiamine thiazolone pyrophosphate (TTPP) on the in vitro and in vivo inhibition of pyruvate dehydrogenase complex (PDHC) from rat cortex and hippocampus were characterized. TTPP decreased PDHC activity in vitro but had no effect in vivo following its direct chronic administr...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Kandiko CT,Smith D,Yamamoto BK

    更新日期:1988-11-15 00:00:00

  • Production of adenosine and nucleoside analogs by the exchange reaction catalyzed by rat liver adenosine kinase.

    abstract::We have previously shown [8] that rat liver adenosine kinase can produce [14C]AMP from [14C]adenosine (Ado) and unlabelled adenosine monophosphate (AMP), in the absence of ATP, by an exchange reaction. In this study, we investigated whether Ado or AMP could be replaced in this exchange reaction by other nucleosides or...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Mimouni M,Bontemps F,Van den Berghe G

    更新日期:1995-11-09 00:00:00

  • Prevention of UVB-induced photoinflammation and photoaging by a polymethoxy flavonoid, nobiletin, in human keratinocytes in vivo and in vitro.

    abstract::Exposure to ultraviolet B (UVB) irradiation induces acute skin inflammation such as erythema (sunburn) and edema, and prostaglandin (PG)E2 in the epidermis plays an important role as its prominent mediator. In the present study, we investigated the effect of nobiletin (5,6,7,8,3',4'-hexamethoxy flavone) from Citrus de...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Tanaka S,Sato T,Akimoto N,Yano M,Ito A

    更新日期:2004-08-01 00:00:00

  • Effects of lead acetate on DNA and RNA synthesis by intact HeLa cells, isolated nuclei and purified polymerases.

    abstract::The effects of lead acetate on DNA and RNA synthesis have been investigated with intact HeLa cells, isolated nuclei, and purified DNA and RNA polymerases. No inhibition of DNA or RNA synthesis in intact cells was found even after exposure to 0.5 mM lead acetate for 18 hr. In contrast, both DNA and RNA synthesis in iso...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Frenkel GD,Middleton C

    更新日期:1987-01-15 00:00:00

  • Poly (ADP-ribose) polymerases inhibitor, Zj6413, as a potential therapeutic agent against breast cancer.

    abstract::Poly (ADP-ribose) polymerases (PARPs) facilitate repairing of cancer cell DNA damage as a mean to promote cancer proliferation and metastasis. Inhibitors of PARPs which interfering DNA repair, in context of defects in other DNA repair mechanisms, can thus be potentially exploited to inhibit or even kill cancer cells. ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Zhou Q,Ji M,Zhou J,Jin J,Xue N,Chen J,Xu B,Chen X

    更新日期:2016-05-01 00:00:00

  • The modulation effect of vitamin E on prostaglandin E2 level and ornithine decarboxylase activity at the promotion phase of lung tumorigenesis in mice.

    abstract::The present study was undertaken to investigate a mechanism of the inhibitory effect of vitamin E in urethane-induced lung tumorigenesis in mice. We assayed ornithine decarboxylase (ODC) activity and the prostaglandin E2 (PGE2) level in lung at 8 weeks after urethane injection (promotion phase). Excessive vitamin E fe...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Yano T,Yano Y,Uchida M,Murakami A,Hagiwara K,Otani S,Ichikawa T

    更新日期:1997-06-01 00:00:00

  • Toxic effects of cobalt in primary cultures of mouse astrocytes. Similarities with hypoxia and role of HIF-1alpha.

    abstract::Cobalt is suspected to cause memory deficit in humans and was reported to induce neurotoxicity in animal models. We have studied the effects of cobalt in primary cultures of mouse astrocytes. CoCl(2) (0.2-0.8mM) caused dose-dependent ATP depletion, apoptosis (cell shrinkage, phosphatidylserine externalization and chro...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Karovic O,Tonazzini I,Rebola N,Edström E,Lövdahl C,Fredholm BB,Daré E

    更新日期:2007-03-01 00:00:00

  • Effect of an aromatase inhibitor, 1,4,6-androstatriene-3,17-dione, on 7,12-dimethylbenz[a]anthracene-induced mammary tumors in the rat and its mechanism of action in vivo.

    abstract::In this study, 1,4,6-androstatriene-3,17-dione (ATD) was demonstrated to cause time-dependent loss of aromatase activity in rat ovarian microsomes in vitro. In vivo, an injection of ATD caused inhibition of ovarian aromatase and reduced estrogen secretion in pregnant mare's serum gonadotropin-primed rats for at least ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Brodie AM,Brodie HJ,Garrett WM,Hendrickson JR,Marsh DA,Tsai-Morris CH

    更新日期:1982-06-01 00:00:00

  • Carboplatin as opposed to cisplatin does not stimulate the expression of the human immunodeficiency virus long terminal repeat sequences.

    abstract::The recombinant plasmid pBHIV1 carrying the long terminal repeat (LTR) of the human immunodeficiency virus 1 (HIV-1), linked to the chloramphenicol acetyl transferase (CAT) gene, was introduced into human and rat fibroblasts. Stable transfectants resistant to geneticin expressed CAT activity from the HIV-1 LTR. It was...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Zoumpourlis V,Kerr DJ,Spandidos DA

    更新日期:1992-02-04 00:00:00

  • Morphine is a substrate of the organic cation transporter OCT1 and polymorphisms in OCT1 gene affect morphine pharmacokinetics after codeine administration.

    abstract::We investigated whether morphine and its pro-drug codeine are substrates of the highly genetically polymorphic organic cation transporter OCT1 and whether OCT1 polymorphisms may affect morphine and codeine pharmacokinetics in humans. Morphine showed low transporter-independent membrane permeability (0.5 × 10⁻⁶ cm/s). ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Tzvetkov MV,dos Santos Pereira JN,Meineke I,Saadatmand AR,Stingl JC,Brockmöller J

    更新日期:2013-09-01 00:00:00

  • Association of galactosamine-induced hepatitis in the rat with hyperhyaluronanaemia and decreased hyaluronan uptake by the isolated, perfused liver.

    abstract::Plasma hyaluronan (HA) concentration and the rate of HA uptake by the isolated, perfused liver were measured in rats treated with saline, D-galactosamine (GaI-NH2, 50 mg/100 g body wt), gadolinium chloride (GdCl3) (0.5 mg/100 g body wt), and GdCl3 + GaI-NH2. GdCl3 was given 24 hr before GaI-NH2 or saline. Plasma L-ala...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Deaciuc IV,Bagby GJ,Spitzer JJ

    更新日期:1993-08-17 00:00:00

  • Positive allosteric modulators as an approach to nicotinic acetylcholine receptor-targeted therapeutics: advantages and limitations.

    abstract::Neuronal nicotinic acetylcholine receptors (nAChR), recognized targets for drug development in cognitive and neuro-degenerative disorders, are allosteric proteins with dynamic interconversions between multiple functional states. Activation of the nAChR ion channel is primarily controlled by the binding of ligands (ago...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Williams DK,Wang J,Papke RL

    更新日期:2011-10-15 00:00:00

  • Homo- and hetero-dimerization of human UDP-glucuronosyltransferase 2B7 (UGT2B7) wild type and its allelic variants affect zidovudine glucuronidation activity.

    abstract::Most human UDP-glucuronosyltransferase (UGT; EC genes contain non-synonymous single nucleotide polymorphisms (nsSNPs) which cause amino acid substitutions. Allelic variants caused by nsSNPs may exhibit absent or reduced enzyme activity. UGT2B7 is one of the most important UGTs that glucuronidates abundant en...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Yuan L,Qian S,Xiao Y,Sun H,Zeng S

    更新日期:2015-05-01 00:00:00

  • Inhibition by suramin of mitochondrial ATP synthesis.

    abstract::Suramin, a drug intensively used in the chemotherapy of African trypanosomiasis and onchocerciasis, is currently being tested in clinical trials for AIDS treatment. Its effects on mitochondrial energy metabolism in mammals were studied. At low concentrations it inhibited ATP synthesis and ATPase activity in submitocho...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Calcaterra NB,Vicario LR,Roveri OA

    更新日期:1988-07-01 00:00:00

  • Cholesteryl ester hydroperoxide formation in myoglobin-catalyzed low density lipoprotein oxidation: concerted antioxidant activity of caffeic and p-coumaric acids with ascorbate.

    abstract::Two diet-derived phenolic acids, caffeic and p-coumaric acids, interplayed with ascorbate in the protection of low density lipoproteins (LDL) from oxidation promoted by ferrylmyoglobin. Ferrylmyoglobin, a two-electron oxidation product from the reaction of metmyoglobin and H2O2, was able to oxidize LDL, degrading free...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Vieira O,Laranjinha J,Madeira V,Almeida L

    更新日期:1998-02-01 00:00:00

  • In vivo conversion of gamma-aminobutyric acid and 1,4-butanediol to gamma-hydroxybutyric acid in rat brain. Studies using stable isotopes.

    abstract::The formation of 4-[1,4-13C]hydroxybutyric acid ([13C]gamma-hydroxybutyric acid; [13C]GHB) in rat brain was studied following intracerebroventricular (i.c.v.) administration of either 4-[1,4-13C]aminobutyric acid ([13C]GABA or 1,4-[1,4-13C]butanediol ([13C]1,4-BD) to awake, freely moving animals. GHB and [13C]GHB were...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Snead OC 3rd,Furner R,Liu CC

    更新日期:1989-12-15 00:00:00

  • Effect of stilbene derivatives on gastric H+, K(+)-ATPase.

    abstract::The effect of naturally occurring hydroxystilbene, 3,3',4,5-tetrahydroxystilbene (piceatanol), and its derivatives on gastric H+, K(+)-ATPase was studied. Piceatanol inhibited H+, K(+)-ATPase in a dose-dependent manner. The 50% inhibition value was 4.3 x 10(-6) M. It was found from the kinetic study that the inhibitio...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Murakami S,Arai I,Muramatsu M,Otomo S,Baba K,Kido T,Kozawa M

    更新日期:1992-11-17 00:00:00

  • Inhibitory effect of quinolone antimicrobial and nonsteroidal anti-inflammatory drugs on a medium chain acyl-CoA synthetase.

    abstract::The inhibitory effects of quinolone antimicrobial agents and nonsteroidal anti-inflammatory drugs on purified mouse liver mitochondrial medium chain acyl-CoA synthetase catalyzing the first reaction of glycine conjugation were examined, using hexanoic acid as a substrate. Enoxacin, ofloxacin, nalidixic acid, diflunisa...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Kasuya F,Hiasa M,Kawai Y,Igarashi K,Fukui M

    更新日期:2001-08-01 00:00:00

  • Structural elements pertinent to the interaction of cyclosporin A with its specific receptor protein, cyclophilin.

    abstract::Cyclophilin (163 amino acids; 17,737 daltons) is a ubiquitous cytosolic protein that specifically binds the potent immunosuppressive drug cyclosporin A (CsA). To characterize the structural details of this interaction, extensive use has been made of two-dimensional (2D) NMR methods. For studies on CsA, these methods a...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Hsu VL,Heald SL,Harding MW,Handschumacher RE,Armitage IM

    更新日期:1990-07-01 00:00:00

  • Autophagic cell death, polyploidy and senescence induced in breast tumor cells by the substituted pyrrole JG-03-14, a novel microtubule poison.

    abstract::JG-03-14, a substituted pyrrole that inhibits microtubule polymerization, was screened against MCF-7 (p53 wild type), MDA-MB231 (p53 mutant), MCF-7/caspase 3 and MCF-7/ADR (multidrug resistant) breast tumor cell lines. Cell viability and growth inhibition were assessed by the crystal violet dye assay. Apoptosis was ev...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Arthur CR,Gupton JT,Kellogg GE,Yeudall WA,Cabot MC,Newsham IF,Gewirtz DA

    更新日期:2007-10-01 00:00:00

  • Formation and retention and biological activity of N10-propargyl-5,8-dideazafolic acid (CB3717) polyglutamates in L1210 cells in vitro.

    abstract::The formation, retention and biological activity of the polyglutamate metabolites of the thymidylate synthase (TS) inhibitor N10-propargyl-5,8-dideazafolic acid (CB3717) has been investigated in L1210 murine leukaemia cells grown in vitro. CB3717 polyglutamates were measured by HPLC using high specific activity 3H-CB3...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Sikora E,Jackman AL,Newell DR,Calvert AH

    更新日期:1988-11-01 00:00:00

  • A review of experimental techniques used for the heterologous expression of nicotinic acetylcholine receptors.

    abstract::Nicotinic acetylcholine receptors (nAChRs) are members of the Cys-loop family of neurotransmitter-gated ion channels, a family that also includes receptors for gamma-aminobutyric acid, glycine and 5-hydroxytryptamine. In humans, nAChRs have been implicated in several neurological and psychiatric disorders and are majo...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Millar NS

    更新日期:2009-10-01 00:00:00