Abstract:
:A technique is described to preserve the pigment found in the bodies and the intestine of some brightly coloured and darkly pigmented benedeniine capsalid monogeneans. Previous studies of these pigmented capsalids have proven difficult because the pigmentation usually disappears when the worms are fixed using preservatives containing concentrations of formalin over 5% and/or ethanol, acetic acid, chromic acid, picric acid and mercuric chloride. The technique developed here uses a fixative comprising glycerol, acetone and formalin (GAF). After fixation under light coverslip compression for three minutes, specimens are transferred to absolute acetone for three minutes and cleared in a mixture of nine parts cedar wood oil and one part absolute acetone before mounting in Canada balsam. Processing must be carried out quickly, as these chemicals will cause the pigments to fade if the specimens are exposed to them for too long. Pigmented benedeniines processed using this technique retain the distribution, intensity and colour observed in live worms. The colour and distribution of pigmentation in monogeneans may be of taxonomic importance and this technique aids preparation of whole-mounts suitable for registration as type-material.
journal_name
Syst Parasitoljournal_title
Systematic parasitologyauthors
Deveney MR,Whittington IDdoi
10.1023/a:1026558405617subject
Has Abstractpub_date
2001-01-01 00:00:00pages
31-5issue
1eissn
0165-5752issn
1573-5192journal_volume
48pub_type
杂志文章abstract::A synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa, Myxosporea, Myxobolidae) described between 2005 and 2013 is presented, including 112 nominal species. For each species the most important morphological and morphometric features are indicated in tabulated format. Included are also accession numbers for mo...
journal_title:Systematic parasitology
pub_type: 杂志文章,评审
doi:10.1007/s11230-014-9484-5
更新日期:2014-05-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00
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更新日期:2016-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2018-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-04-01 00:00:00
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更新日期:2013-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-02-01 00:00:00
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journal_title:Systematic parasitology
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
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journal_title:Systematic parasitology
pub_type: 杂志文章
doi:10.1023/a:1020414023652
更新日期:2002-10-01 00:00:00