Abstract:
:A practical combination of comparative modeling and NMR spectroscopy was used to generate a three-dimensional structure of the response regulator protein, Spo0F. The backbone structure obtained compares to the Spo0F Y13S mutant X-ray structure with an rmsd of 2.0 A. We provide results which suggest that structures obtained by this method are suitable for drug discovery. The results of the GRID and DOCK methods as applied to the model and X-ray structures of Spo0F are remarkably similar and tend to suggest the same design conclusions. This trend is illustrated by these same techniques applied to two experimentally derived structures of the analogous protein, CheY, which exhibit a pairwise rmsdBB on the same order as that found for the two Spo0F structures.
journal_name
J Med Chemjournal_title
Journal of medicinal chemistryauthors
Podlogar BL,Leo GC,McDonnell PA,Loughney DA,Caldwell GW,Barrett JFdoi
10.1021/jm970156isubject
Has Abstractpub_date
1997-10-10 00:00:00pages
3453-5issue
21eissn
0022-2623issn
1520-4804pii
jm970156ijournal_volume
40pub_type
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