Abstract:
:The role of GAS6 antisense RNA 1 (GAS6-AS1) in clear cell renal cell carcinoma (ccRCC) remains unclear. The aim of the present study was to investigate the role and molecular mechanisms of GAS6-AS1 in the progression of ccRCC. GAS6-AS1 was found to be upregulated in ccRCC tissues and cell lines, and patients with high GAS6-AS1 expression levels exhibited a poor prognosis. Small interfering (si)RNA GAS6-AS1 inhibited the activity, colony formation, invasiveness and glycolysis of OSRC-2 and SW839 cells, while GAS6-AS1 overexpression promoted these functions. Moreover, si-GAS6-AS1 increased the phosphorylation level of AMP-activated protein kinase (AMPK) and decreased that of mTOR, as well as decreasing proliferating cell nuclear antigen (PCNA), MMP-2 and hexokinase-2 (HK2) expression, which were reversed by inhibiting AMPK or mTOR. In addition, the silencing of GAS6-AS1 suppressed the growth of xenografted tumors and attenuated the expression of PCNA, MMP-2 and HK2 in tumor tissues. These findings conclude that GAS6-AS1 regulated the proliferation, invasiveness and glycolysis of ccRCC cells by regulating the AMPK/mTOR signaling pathway, and suggest that GAS6-AS1 may be a potential therapeutic target for ccRCC.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Guo X,Li H,Zhang M,Li Rdoi
10.3892/ol.2021.12988keywords:
["AMP-activated protein kinase\/mTOR signaling pathway","GAS6 antisense RNA 1","clear cell renal cell carcinoma","glycolysis","proliferation"]subject
Has Abstractpub_date
2021-10-01 00:00:00pages
727issue
4eissn
1792-1074issn
1792-1082pii
OL-0-0-12988journal_volume
22pub_type
杂志文章相关文献
文献大全abstract::The metastatic spread of tumor cells is one of the most common causes of mortality in cancer patients. The elucidation of the molecular mechanisms that underlie the formation of metastatic colonies has been one of the major objectives of cancer research. Organ-specific colonization of cancer cells is a significant and...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.432
更新日期:2012-01-01 00:00:00
abstract::There is currently no standardized therapy available for metastatic breast cancer in patients with aromatase inhibitor (AI)-resistant breast cancer. We conducted a prospective study to examine the efficacy and safety of high-dose toremifene (TOR) treatment for the first-line treatment of metastatic breast cancer follo...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.449
更新日期:2012-01-01 00:00:00
abstract::Small cell carcinoma of the uterine cervix (SCCUC) is rare and its biologic behavior is aggressive. To analyze prognostic factors and determine optimal therapy in patients with International Federation of Gynecology and Obstetrics (FIGO) stage IB1-IIA SCCUC, we retrospectively reviewed 96 patients (14 patients treated...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.439
更新日期:2012-01-01 00:00:00
abstract::Urinary incontinence is a major complication following radical prostatectomy. The aim of the present study was to assess the association between urinary continence following laparoscopic radical prostatectomy (LRP) and various factors measured using real-time intraoperative transrectal ultrasonography (TRUS). Patients...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.446
更新日期:2012-01-01 00:00:00
abstract::The diagnosis of synchronous multiple primary lung cancer (SMPLC) remains a formidable challenge. The aim of the present study was to identify useful clues for the clinical diagnosis of SMPLC, in particular for the early stages. The medical records of 10 patients diagnosed with SMPLC with different histological types ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.452
更新日期:2012-01-01 00:00:00
abstract::Liposarcomas are separated into clinicopathological entities by a characteristic morphological spectrum and distinctive genetic changes. Myxoid liposarcoma (MLS) represents one such entity with specific chromosomal translocations leading to the generation of fusion genes, the human translocation liposarcoma (TLS)-CCAA...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.480
更新日期:2012-02-01 00:00:00
abstract::Humoral hypercalcemia of malignancy is frequently observed in patients with solid tumors. However, few instances have been described involving patients with gynecological malignancies. We report a case of endometrioid carcinoma of the uterine corpus in a patient who initially presented with hypercalcemia. The elevated...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.475
更新日期:2012-02-01 00:00:00
abstract::The oncolytic virus is expected to proliferate in and destroy tumor cells. The virus is also thought to generate antitumor immunity. Virally infected tumor cells express viral antigens on their surfaces. Such tumor cells or their fragments would be taken up by antigen-presenting cells (APCs) together with tumor-associ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.467
更新日期:2012-02-01 00:00:00
abstract::The functional profile of natural killer (NK) cells has been reported to be lower in auto-immune haemolytic anaemia (AIHA). In this study, we report a comparative analysis of peripheral blood mononuclear cells (PBMNCs) and the in vitro expansion of NK cells in a patient with AIHA and cancer, with that of other cancer ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.498
更新日期:2012-02-01 00:00:00
abstract::Multidrug resistance is a serious obstacle encountered in leukemia treatment. Previous studies have found drug resistance in human leukemia is mainly associated with overexpression of the multidrug resistance gene 1 (MDR1). The aim of the present study was to investigate the modulation of P-glycoprotein expression by ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.500
更新日期:2012-02-01 00:00:00
abstract::This study aimed to examine the role of chronic restraint stress (RS) on oral squamous cell carcinomas induced by 4-nitroquinoline-1-oxide (4-NQO) in CF-1 mouse tongues, measured by the expression of argyrophilic staining of nucleolar organizer regions (AgNOR). Thirty one samples of lingual epithelial tissue of CF-1 m...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.514
更新日期:2012-03-01 00:00:00
abstract::Angiomyolipoma (AML) is a benign mesenchymal tumor composed of variable proportions of adipose tissue, spindle and epithelioid smooth muscle cells and abnormal thick-walled blood vessels. Approximately one-third of epithelioid AML (EAML) cases have been reported to have metastasis. Thus, it would be of interest to ide...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.554
更新日期:2012-03-01 00:00:00
abstract::The objective of this study was to evaluate the efficacy and safety of neoadjuvant chemotherapy (NAC) with paclitaxel combined with carboplatin and cisplatin prior to laparoscopic radical hysterectomy and pelvic lymphadenectomy. A total of 19 patients who had bulky cervical cancer of International Federation of Gyneco...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.529
更新日期:2012-03-01 00:00:00
abstract::Cases of colo-ileal carcinoma (CIC), defined as intestinal carcinoma involving the right-sided colon and the ileum, are rarely encountered. The aim of this study was to elucidate the clinicopathological characteristics, which have been poorly understood, in such cases. A total of 16 CICs were examined histologically a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.538
更新日期:2012-03-01 00:00:00
abstract::The Wilm's tumor gene (WT1), encoding a transcription factor that modulates the expression of certain genes that are involved in proliferation and apoptosis, is overexpressed in numerous solid tumors. WT1 is important for cell proliferation and in the diagnosis of melanoma. The objectives of this study were to investi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.578
更新日期:2012-04-01 00:00:00
abstract::Metastases account for 90% of lung cancer mortalities, frequently target the skeleton and lead to rapid deterioration in quality of life. The molecular mechanism underlying bone metastases is largely unknown. Development of xenograft mouse models, such as the severe combined immunodeficient (SCID) CB-17 mouse and the ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.586
更新日期:2012-04-01 00:00:00
abstract::We retrospectively reviewed the medical records of 32 chemonaïve patients with either breast, lung or prostate cancer, who were treated with docetaxel (DOC) monotherapy, and evaluated whether the proportion of peripheral blood monocytes was capable of predicting the occurrence of neutropenia following chemotherapy. In...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.556
更新日期:2012-04-01 00:00:00
abstract::Apoptosis inhibitor 5 (API-5) is a 55 kDa nuclear protein with potent anti-apoptotic signaling in tumor cells in vitro. In this study, we analyzed the expression of the API-5 protein in vivo in a broad spectrum of human carcinomas, including those of the colon, lung, liver, kidney, pancreas, stomach and esophagus usin...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.593
更新日期:2012-04-01 00:00:00
abstract::Chemotherapy with FOLFOX, which is a combination of 5-fluorouracil (5-FU)/leucovorin (LV) and oxaliplatin, has been used worldwide for the treatment of metastatic colorectal cancer patients. The aim of this study was to examine the candidates for predictors of the efficacy of the FOLFOX treatment regimen in colorectal...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.598
更新日期:2012-05-01 00:00:00
abstract::The aim of this study was to determine the level of expression of Mina53 in human cholangiocarcinoma (CCA) and to explore the role of Mina53 in carcinogenesis and tumor progression and its clinical significance in CCA. The level of expression of Mina53, p53 and Ki67 was investigated by immunohistochemistry in 69 surgi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.620
更新日期:2012-05-01 00:00:00
abstract::Piceatannol (3,3',4,5'-tetrahydroxy-trans-stilbene; Pice), found in a variety of plant sources including grapes, red wine, peanuts and rhubarb, is known as a metabolite and analog of Resveratrol (3,5,4'-trihydroxy-trans-stilbene; Res) and has higher bioactivity than Res. To explore the mechanism of DNA damage induced ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.597
更新日期:2012-05-01 00:00:00
abstract::Evidence suggests that different types of mutation in gastrointestinal stromal tumours (GISTs) correlate with different response rates to imatinib (Glivec, STI571). The purpose of this study was to explore the sensitivity of the PDGFRA(L839P) mutant, a novel gain-of-function mutation isoform related to GISTs, to imati...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.599
更新日期:2012-05-01 00:00:00
abstract::Sorafenib and sunitinib are multi-kinase inhibitors with antitumor activity in patients with advanced renal cell carcinoma (RCC). Several studies have evaluated the effect of sorafenib/sunitinib in combination with chemotherapeutic agents in different types of tumor. However, few studies have addressed the activity of...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.662
更新日期:2012-06-01 00:00:00
abstract::Acquisition of anoikis resistance is a prerequisite for the metastasis of hepatocellular carcinoma (HCC) cells. Activation of growth factor signaling pathways and rearrangement of the cytoskeleton have been reported as vital steps in this process. However, key molecules involved in anoikis resistance remain to be dete...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.658
更新日期:2012-06-01 00:00:00
abstract::We report the case of a woman who developed acute thrombocytopenia with hemorrhagic diathesis during adjuvant treatment of colorectal adenocarcinoma with oxaliplatin, 5-fluorouracil and leucovorin. A 55-year-old woman started adjuvant chemotherapy with oxaliplatin, 5-fluorouracil and leucovorin (mFOLFOX6). Prior to st...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.653
更新日期:2012-06-01 00:00:00
abstract::Cyclin-dependent kinase 6 (Cdk6) controls the cell cycle and aberrant expression of Cdk6 is involved in cancer progression. The aim of this study was to investigate the role of Cdk6 in bladder cancer development. Cdk6 expression was examined in 31 cases of bladder cancer and 29 tissues adjacent to bladder transitional...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.695
更新日期:2012-07-01 00:00:00
abstract::Ethylenediaminetetraacetic acid-dependent pseudothrombocytopenia (EDTA-PTCP) is an in vitro phenomenon of EDTA-induced platelet aggregation at room temperature. This phenomenon consists of platelet clumping due to anti-platelet antibodies in blood anticoagulated with EDTA. It has been reported in patients with various...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.675
更新日期:2012-07-01 00:00:00
abstract::BRCA1 overexpression and phosphoinositide 3-kinase (PIK3CA) pathway activation are involved in the resistance to DNA damaging agents. Thus, we hypothesized that BRCA1 protein expression and activating PIK3CA mutations are potential tumor biomarkers for the chemotherapeutic response to doxorubicin/cyclophosphamide plus...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.697
更新日期:2012-07-01 00:00:00
abstract::The aim of this study was to investigate the changes in the uptake function of the liver in rats with obstructive jaundice prior to and following relief from obstructive jaundice, and to investigate whether superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance imaging (MRI) could be used to assess liver upta...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.729
更新日期:2012-08-01 00:00:00
abstract::Mantle cell lymphoma (MCL) of the prostate, either primary or secondary, is a rare entity. This case report examines an 83-year-old male who complained of not only nocturia (2-3 times), but also frequency and urgency of urination. The maximal urinary flow rate was 4.1 ml/sec. A transrectal ultrasound-guided prostate b...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.732
更新日期:2012-08-01 00:00:00