Abstract:
:The conventional storage temperature of 16-18 °C provides optimal conditions for the preservation of boar sperm quality, which are extremely cold sensitive cells. On the other hand, however, it requires the addition of antibiotics to inhibit bacterial growth. Rising numbers of antibiotic resistant bacteria call for alternatives to this conventional storing method. As potential alternative, three different bacteriocin candidates with known bacteriolytic activity against E. coli were examined on possible negative effects concerning the sperm quality and on their impact on bacterial growth of E. coli ILSH 02692 in BTS-extended semen w/o antibiotics. Although the lower concentrations (0.01 and 0.25%) of all bacteriocins did not show any impact on the quality of the semen, the higher concentrations (0.5 and 1.0%) of two bacteriocins led to a significant (P < 0.05) reduction in several sperm quality characteristics. The bacteriocin 860/1c after AMS/dialysis did not affect the sperm quality in any of the tested concentrations and in all tested extenders (BTS, MIII, Androstar Premium and Androhep all w/o antibiotics) at 16 °C as well as at 6 °C. This bacteriocin reduced growth of E. coli ILSH 02692 in BTS-extended semen by 50% compared to the control w/o bacteriocin. Furthermore, a preliminary insemination trial indicated no impact of the selected bacteriocin on fertility. These promising results show that the application of bacteriocins in liquid-preserved semen is a feasible possibility in the future.
journal_name
Theriogenologyjournal_title
Theriogenologyauthors
Jakop U,Hensel B,Orquera S,Rößner A,Alter T,Schröter F,Grossfeld R,Jung M,Simmet C,Schulze Mdoi
10.1016/j.theriogenology.2021.08.004keywords:
["Antimicrobial resistance","Bacteriocins","Boar","Boar semen preservation","Boar sperm"]subject
Has Abstractpub_date
2021-10-01 00:00:00pages
163-172eissn
0093-691Xissn
1879-3231pii
S0093-691X(21)00257-0journal_volume
173pub_type
杂志文章相关文献
文献大全abstract::Sertoli cells provide nutrients and support for germ cell differentiation and maintain a stable microenvironment for spermatogenesis. Comprehensive identification of Sertoli cellular proteins is important in understanding spermatogenesis. In this study, we performed an integrative analysis of the proteome and phosphop...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2021.04.013
更新日期:2021-04-27 00:00:00
abstract::The present study was carried out to compare the proteomic profiles of spermatogenic cells of crossbred and zebu cattle in an effort to understand the possible reasons for a higher incidence of sub-fertility in crossbred bulls. The spermatogenic cells collected from the testes of pre-pubertal (6 mo) and adult (24 mo) ...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2021.04.012
更新日期:2021-04-24 00:00:00
abstract::Follicular atresia is primarily caused by granulosa cell (GC) apoptosis, although the mechanisms are largely unknown. Ufmylation is a recently identified ubiquitin-like post-translational modifier that plays an important role in cell proliferation and apoptosis. The purpose of this study was to investigate the effects...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2021.04.009
更新日期:2021-04-22 00:00:00
abstract::Commercially available intravaginal progesterone (P4) devices differ in shape, surface area and P4 load, which may affect the resulting pregnancy per AI (P/AI) following timed-AI (TAI). The objective of this study was to compare two intravaginal P4 devices on estrus rate, follicular dynamics and P/AI in beef cattle su...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2021.03.023
更新日期:2021-07-01 00:00:00
abstract::During capacitation, proteins in boar sperm are released to maintain the stability of their own state and membrane structure. No studies have analyzed the differences between retained proteins and released proteins during sperm capacitation. In the present study, a Transwell chamber and polycarbonate membrane were use...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2021.03.010
更新日期:2021-07-01 00:00:00
abstract::The aim of this study was to evaluate the outcomes of an early resynchronization protocol (Resynch) initiated at different timepoints after timed artificial insemination (TAI) and with unknown pregnancy status. Holstein cows (n = 164) were submitted to the following TAI protocol: D0, insertion of an intravaginal proge...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2021.03.009
更新日期:2021-06-01 00:00:00
abstract::Small ubiquitin-like modifier 2 (SUMO2) is a small protein that modulates the stability and activity of other proteins. Although a variety of activities have been attributed to SUMO2, its function in preimplantation embryos is still obscure. We first explored the expression of SUMO2 protein in early embryos, and showe...
journal_title:Theriogenology
pub_type: 杂志文章,评审
doi:10.1016/j.theriogenology.2021.01.019
更新日期:2021-05-01 00:00:00
abstract::The current study was conducted to evaluate the effect of dietary camphor levels as a medicinal feed additive to improve semen quality, antioxidant capacity, reproductive hormones, and reproduction performance in roosters. For this purpose, thirty-five 29-wk-old Ross 308 broiler breeder roosters randomly were assigned...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2021.02.002
更新日期:2021-05-01 00:00:00
abstract::Interspecific germline chimerism mediated by transplantation of primordial germ cells (PGCs) of wild species to domestic hosts promises the conservation of wild birds. Cryopreservation of avian eggs and embryos is impracticable, and currently only frozen PGCs enable conservation of both the male and female descendants...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2021.02.010
更新日期:2021-04-15 00:00:00
abstract::We hypothesized that feeding a Saccharomyces cerevisiae fermentation product (SCFP) from -4 through +7 wk (calving = Day 0) facilitates early first postpartum ovulation and alters blood and follicular fluid concentrations of glucose, beta-hydroxybutyrate (BHB), free fatty acids (FFA), and steroid hormones favorable to...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2021.01.013
更新日期:2021-04-01 00:00:00
abstract::The in vitro propagation and differentiation of spermatogonial stem cells (SSCs) has many potential applications within reproductive science and medicine. We established a two-dimensional (2D) cell culture system to proliferate and differentiate prepubertal mouse SSCs as a model capable of maximizing on a small number...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.12.024
更新日期:2021-03-01 00:00:00
abstract::Ovary development of Chinese sturgeon (Acipenser sinensis) in controlled breeding has been reported to respond to dietary lipid levels. However, the corresponding molecular regulatory mechanism about ovary development of Chinese sturgeon is still unclear. To elucidate the molecular mechanism of vitellogenic deposition...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.12.023
更新日期:2021-03-01 00:00:00
abstract::The present study aimed to elucidate the developmental kinetics, growth potential, and viability of bovine embryos produced in vitro with sexed semen. Bovine oocytes were fertilized in vitro using unsorted and X-sorted semen from the same Holstein bulls, and the kinetics of in vitro development were continuously monit...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.12.001
更新日期:2021-02-01 00:00:00
abstract::In cattle, in vitro embryo production (IVEP) is an important reproductive biotechnology responsible for the rapid expansion of the Senepol breed in our country. This breed has shown important results when used in crossbreeding and estimate IVEP in Senepol based on seminal analysis would be valuable for the semen cryop...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.11.019
更新日期:2021-02-01 00:00:00
abstract::A duration of parturition beyond 300 min negatively impacts the health of the sow and the survival of piglets during parturition. Hence, oxytocin is widely used to speed up the parturition. However, oxytocin's negative side effects raise the need of finding alternative treatments such as those already implemented in h...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.11.013
更新日期:2021-02-01 00:00:00
abstract::Placenta-specific 8 (PLAC8) is one of the placenta-regulatory genes which is highly conserved among eutherian mammals. However, little is known about its expression in equine placenta (chorioallantois; CA and endometrium; EN) during normal and abnormal pregnancy. Therefore, the current study was designed to 1) elucida...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.10.041
更新日期:2021-01-15 00:00:00
abstract::While intracytoplasmic sperm injection (ICSI) is an asset in human Assisted Reproduction Technologies (ART), its outcomes, in terms of blastocyst, is still unacceptably low in ruminants. The picture typically found in ICSI derived bovine and ovine embryos is an asymmetry between a high activation rate, marked by a pro...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.10.008
更新日期:2021-01-01 00:00:00
abstract::The aim of this study was to investigate the molecular mechanisms of arginine (Arg) on follicular development of acute feed-restricted ewes during the luteal phase. From day 6 of the estrous cycle, 24 multiparous Hu sheep were randomly assigned into three groups: control group (a maintenance diet; n = 6), feed restric...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.09.002
更新日期:2020-12-01 00:00:00
abstract::Breeding a bitch with two different sires during a single estrous cycle has the potential to facilitate rapid genetic gain and improve reproductive performance within a canine breeding colony. There is limited data regarding the factors that contribute to the success of dual-sired litters in domestic dogs, and only an...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.08.030
更新日期:2020-12-01 00:00:00
abstract::Oocytes of better quality and developmental competence are highly demanded, which is affected by many intrinsic and external factors, including environmental pollutants. We have previously demonstrated that 7, 12-dimethylbenz [a]anthracene (DMBA) reduces the developmental competence of porcine oocytes, by desynchroniz...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.08.020
更新日期:2020-11-01 00:00:00
abstract::To more clearly understand the equine gonadotrope response to kisspeptin and gonadotropin releasing hormone (GnRH), peripheral LH and FSH were quantified in diestrous mares after treatment with either equine kisspeptide (eKp-10, 0.5 mg iv), GnRH (25 μg iv), or a combination thereof every 4 h for 3 days. The following ...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.07.031
更新日期:2020-11-01 00:00:00
abstract::Recently, it was reported that hamster sperm hyperactivation is regulated by extracellular Na+. Two types of catalytic Na+/K+-ATPase (NKA) α subunits (α1 and α4) are present in spermatozoa. In this work, the contribution of these NKA subunits to the regulation of hamster sperm hyperactivation was investigated using th...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.06.028
更新日期:2020-11-01 00:00:00
abstract::Once in the female reproductive tract, spermatozoa undergo several modifications to acquire their complete fertilizing ability. Interactions between the oviductal fluid (OF) and gametes contribute to a successful fertilization. Recently, oviductal extracellular vesicles have been identified as an important part of the...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.05.043
更新日期:2020-10-01 00:00:00
abstract::In captive rhinoceros stillbirth and perinatal death are recorded at a rate of 6-17% in the various species. At the same time there is a substantial lack of knowledge on rhinoceros parturition. Yet, predicting parameters for birth and progress of parturition are fundamental for the recognition of dystocia and perinata...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.06.035
更新日期:2020-10-15 00:00:00
abstract::Primary uterine inertia (PUI) is the most common type of dystocia in dogs. We hypothesized that PUI develops because of lower than normal expression of the basic contractile elements in the uterus, i.e., smooth muscle (SM) α- and γ-actin and SM-myosin, and that the expression of these proteins is influenced by the num...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.06.033
更新日期:2020-10-15 00:00:00
abstract::Leonurine (LEO) is pseudoalkaloid that has been isolated from motherwort. It has been found to have various biological activities, including an antioxidant capacity. This study aimed to confirm whether LEO could be used in porcine in vitro culture (IVC) medium for its antioxidant effect and related molecular mechanism...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.06.038
更新日期:2020-10-15 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) has been extensively characterized as an environmental sensor with major roles in xenobiotic-induced toxicity. Evidence is accumulating that these functions serve as adaptive mechanisms overlapping its physiological roles. We previously described a critical role of constitutive AhR ...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.06.037
更新日期:2020-10-15 00:00:00
abstract::The aim was to develop a program for resynchronization of ovulation (ReBreed21) that allowed reinsemination of non-pregnant Bos indicus heifers every 21 d using timed AI (TAI) without the need for detection of estrus. The Rebreed21 program begins 12 d after previous TAI (Day 0) by inserting an intravaginal P4 implant ...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.04.021
更新日期:2020-10-01 00:00:00
abstract::Heat stress causes subfertility in cattle by inducing alterations in steroidogenic capacity, follicular function and ovulation defects, which eventually negatively affect oocyte quality and embryo survival. Here, the effects of short, moderate temperature elevation during IVM, on embryo yield, and on the expression of...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.06.039
更新日期:2020-10-15 00:00:00
abstract::In rats, birth timing is affected by changes in the light schedule until the middle of the pregnancy period. This phenomenon can be used to control birth timing in the animal industry and/or clinical fields. However, changes in the light schedule until the middle of the pregnancy period can damage the fetus by affecti...
journal_title:Theriogenology
pub_type: 杂志文章
doi:10.1016/j.theriogenology.2020.05.032
更新日期:2020-09-15 00:00:00