Abstract：
:Membrane traffic is an important regulator of cell migration through the endocytosis and recycling of cell surface receptors such as integrin heterodimers. Intracellular nanovesicles (INVs) are transport vesicles that are involved in multiple membrane trafficking steps, including the recycling pathway. The only known marker for INVs is tumor protein D54 (TPD54/TPD52L2), a member of the TPD52-like protein family. Overexpression of TPD52-like family proteins in cancer has been linked to poor prognosis and an aggressive metastatic phenotype, which suggests cell migration may be altered under these conditions. Here, we show that TPD54 directly binds membrane and associates with INVs via a conserved positively charged motif in its C terminus. We describe how other TPD52-like proteins are also associated with INVs, and we document the Rab GTPase complement of all INVs. Depletion of TPD52-like proteins inhibits cell migration and invasion, while their overexpression boosts motility. We show that inhibition of migration is likely due to altered recycling of α5β1 integrins in INVs.
journal_name
J Cell Bioljournal_title
The Journal of cell biologyauthors
Larocque G,Moore DJ,Sittewelle M,Kuey C,Hetmanski JHR,La-Borde PJ,Wilson BJ,Clarke NI,Caswell PT,Royle SJdoi
10.1083/jcb.202009028subject
Has Abstractpub_date
2021-10-04 00:00:00issue
10eissn
0021-9525issn
1540-8140pii
212493journal_volume
220pub_type
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