Abstract:
:A method is described that permits the generation of four isolated blastomeres after embryo splitting of murine four-cell eggs and the subsequent chromosomal analysis of one of the obtained 1/4-blastomeres. According to the karyograms obtained, embryos can be selected for reimplantation and furthermore triplicated via the embryo splitting procedure. By employing the described experimental setup, it is possible specifically to produce trisomy 16----2n aggregation chimeras as a postnatal model system of human Down's syndrome. The design can also be used for embryo sexing in stock farming and the selective reproduction of sexed farm animals via embryo transfer. Furthermore the application of blastomere karyotyping in human genetic counseling is discussed for the descendants of carriers of Robertsonian translocations. In addition the reported method could be employed for the genotypic identification of early homozygous embryonic stages from persons carrying frequent recessive mutations. The proposed design could, therefore, widen the spectrum of prenatal diagnosis.
journal_name
Hum Genetjournal_title
Human geneticsauthors
Bacchus C,Buselmaier Wdoi
10.1007/BF00273646subject
Has Abstractpub_date
1988-12-01 00:00:00pages
333-6issue
4eissn
0340-6717issn
1432-1203journal_volume
80pub_type
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