Abstract:
:A novel, Gram-stain-negative, non-motile and rod-shaped bacterium, designated YH5T, was isolated from the YonghuCo wetland on the Tibetan Plateau. The strain was able to grow optimally with 1 % (w/v) NaCl and tolerated up to 3 % NaCl. Growth occurred at pH 6-9 (optimum pH 7) and 10-37 °C (optimum 30 °C). Vitamins were not required for growth. The major polar lipid of strain YH5T was phosphatidylethanolamine. The predominant respiratory quinone was menaquinone 6 (MK-6). The major fatty acids were iso-C15 : 0, C16 : 0 10-methyl and/or iso-C17 : 1ω9c, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH. Genome sequencing revealed a genome size of 2.74 Mbp and a G+C content of 33.3 mol%. Analysis of 16S rRNA gene sequences showed that strain YH5T belonged to the genus Flavobacterium, with the closest neighbours Flavobacterium luticocti xz20T (96.7 % similarity), Flavobacterium jejuense EC11T (96.4 %), Flavobacterium jumunjinense HME7102T (95.9 %) and Flavobacterium dongtanense LW30T (95.6 %). DNA-DNA relatedness between strain YH5T and the closest phylogenetically related strain F. luticocti xz20T was 27.0 %. Strain YH5T was clearly distinguished from the reference type strains based on phylogenetic analysis, DNA-DNA hybridization, fatty acid composition and a range of physiological and biochemical characteristics. Based on its phenotypic and chemotaxonomic characteristics, strain YH5T is classified as a representative of a novel species of the genus Flavobacterium, for which the name Flavobacterium tibetense sp. nov. is proposed. The type strain is YH5T (=CICC 24247T=KCTC 62174T).
journal_name
Int J Syst Evol Microbiolauthors
Phurbu D,Lu H,Xue X,Tian Y,Pema Y,Ma C,Xing Pdoi
10.1099/ijsem.0.003124subject
Has Abstractpub_date
2019-01-01 00:00:00pages
165-170issue
1eissn
1466-5026issn
1466-5034journal_volume
69pub_type
杂志文章abstract::To investigate the genetic diversity of the genus Arthrospira and to compare it with other cyanobacteria, sequences of 670 nt from the phycocyanin operon were determined for 23 natural, cultivated or commercial strains of Arthrospira and compared with sequences from 20 other non-Arthrospira cyanobacterial strains. The...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-52-3-861
更新日期:2002-05-01 00:00:00
abstract::Two yellow-pigmented, Gram-stain-positive, aerobic, motile, short rod-shaped bacteria were isolated from natural teak tree rhizosphere soil and their taxonomic positions were determined by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strains TG-S248(T) and TG-S240 formed a disti...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.014373-0
更新日期:2010-06-01 00:00:00
abstract::The results of 16S rRNA, gyrB and catA gene sequence comparisons and reasserted DNA-DNA hybridization unambiguously proved that Rhodococcus jialingiae Wang et al. 2010 and Rhodococcus qingshengii Xu et al. 2007 represent a single species. On the basis of priority R. jialingiae must be considered a later synonym of R. ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.059097-0
更新日期:2014-01-01 00:00:00
abstract::A Gram-positive, slightly acid-alcohol-fast, carbon monoxide-oxidizing bacterium, strain Y2(T), was isolated from a soil sample collected from a roadside in Seoul, Korea. On the basis of 16S rRNA gene sequence comparative analyses, strain Y2(T) was shown to belong to the genus Tsukamurella and was most closely related...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.005959-0
更新日期:2009-06-01 00:00:00
abstract::Several strains of sulfate-reducing bacteria were isolated from marine sediments recovered from Hann Bay (Senegal). All were related to members of the genus Desulfovibrio. A strictly anaerobic, mesophilic and moderately halophilic strain designated BLaC1T was further characterized. Cells of strain BLaC1T stained Gram-...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001997
更新日期:2017-09-01 00:00:00
abstract::Previously, analysis of 16S rDNA sequences placed a newly discovered lineage of bacterial symbionts of arthropods in the 'Bacteroidetes'. This symbiont lineage is associated with a number of diverse host reproductive manipulations, including induction of parthenogenesis in several Encarsia parasitoid wasps (Hymenopter...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.02957-0
更新日期:2004-05-01 00:00:00
abstract::The taxonomic position of a lemon-yellow-pigmented actinobacterium, strain JF-6T, isolated from Aurelia aurita, the moon jellyfish, collected from the Bay of Bengal coast, Kanyakumari, India, was determined using a polyphasic approach. The strain had phenotypic and chemotaxonomic properties that were consistent with i...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001407
更新日期:2016-11-01 00:00:00
abstract::A Gram-stain-negative, orange-pigmented, non-spore-forming, non-motile, aerobic, rod-shaped bacterial strain, designated CAU 1131T, was isolated from reclaimed land. Strain CAU 1131T grew optimally at 30 °C and at pH 6.5 in the presence of 4 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed t...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001590
更新日期:2017-01-01 00:00:00
abstract::A yellow-pigmented, rod-shaped, Gram-staining-negative, aerobic bacterial strain, designated CJ7(T), was isolated from a freshwater lake at Chung-Ang University in Anseong, South Korea. Results from 16S rRNA gene sequence analysis revealed that the isolate was phylogenetically affiliated with members of the genus Flav...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.007955-0
更新日期:2009-07-01 00:00:00
abstract::A novel strain, designated W-15(T), was isolated from the gut of a long-horned beetle, Massicus raddei, collected in South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the suborder Micrococcineae. Strain W-15(T) was most closely related to Luteimicrobium album RI1...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.048991-0
更新日期:2014-04-01 00:00:00
abstract::A polyphasic taxonomic study was carried out on strain EBR-4-2T isolated from a biofilm reactor in Korea. Cells of the strain were Gram-stain-positive, non-spore-forming, non-motile and rod-shaped. Comparative 16S rRNA gene sequence studies showed the clear affiliation of this strain to the Actinobacteria, and it had ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001769
更新日期:2017-05-01 00:00:00
abstract::The species Desulfotomaculum auripigmentum is reclassified as Desulfosporosinus auripigmenti corrig., comb. nov. on the basis of morphological and physiological traits, phylogenetic position and chemotaxonomic properties. Characteristics supplementary to those provided in the original description reveal that the type ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.02526-0
更新日期:2003-09-01 00:00:00
abstract::Three novel micro-organisms, designated strains OMB115(T), OMB118 and OMB120, were isolated from dental plaque from golden hamsters fed with a high-carbohydrate diet. The three strains were Gram-positive, facultatively anaerobic rods that lacked catalase activity. Analysis of their partial 16S rRNA gene sequences indi...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65296-0
更新日期:2008-01-01 00:00:00
abstract::A novel, moderately halophilic, rod-shaped bacterium, designated strain YIM D17(T), was isolated from a sample of sediment from a salt mine in Yunnan, south-western China. The taxonomy of strain YIM D17(T) was investigated using a polyphasic approach. Strain YIM D17(T) was Gram-stain-negative, strictly aerobic and non...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.025502-0
更新日期:2012-02-01 00:00:00
abstract::An orange-pigmented bacterium, designated strain 13-9-B8T, was isolated from a seawater sample collected at Marado, Jeju Island, South Korea. The novel strain was Gram-staining-negative, non-motile, non-gliding, rod-shaped and aerobic. A phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain c...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.000435
更新日期:2015-10-01 00:00:00
abstract::A novel actinomycete, strain S187(T), was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0-9 % NaCl and at pH 6.0-9.0 and 10-45 degrees C. The cell wall of strain S187(T) contained the isomer ll-diaminopimelic acid as the diagnostic diamino ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.009878-0
更新日期:2009-11-01 00:00:00
abstract::Strain KIT 00200-70066-1(T) was isolated from the sputum of a patient with pulmonary disease. Cells of the strain were Gram-variable, facultatively anaerobic, motile, spore-forming rods and formed colourless to white colonies on tryptic soy agar at 30 °C and pH 7. The pathogenicity of the strain is not k...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.017137-0
更新日期:2010-10-01 00:00:00
abstract::Four strains of a hitherto unrecognized gram-positive, catalase-negative, facultatively anaerobic, rod-shaped bacterium isolated from human sources were characterized using phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing demonstrated that the bacterium represents a new subline within t...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-51-6-2081
更新日期:2001-11-01 00:00:00
abstract::A Gram-stain-positive, rod-shaped, yellow, non-motile, non-spore-forming, strictly aerobic bacterial strain, designated MW 10(T), was isolated from seawater of the Bay of Bengal, India, and was subjected to a polyphasic taxonomic study. Analysis of the 16S rRNA gene sequence revealed that strain MW 10(T) showed highes...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.023184-0
更新日期:2011-08-01 00:00:00
abstract::A non-motile, rod-shaped, red-pigmented bacterium, designated strain BZ33r(T), was isolated from soil of an industrial site. Cells stained Gram-negative and were aerobic and psychrophilic, showing good growth at 1-20 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ33r(T) was related to ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.023465-0
更新日期:2011-04-01 00:00:00
abstract::A novel aerobic, Gram-negative bacterial strain, designated 5IX/A01/131(T), was isolated from waters in the coastal north-western Mediterranean Sea. The cells were motile, straight rods, 1.6 microm long and 0.5 microm wide, and formed cream colonies on marine 2216 agar. The G+C content of the genomic DNA was 57 mol %....
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65685-0
更新日期:2008-11-01 00:00:00
abstract::A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated GYSW-23(T), was isolated from seawater off Geoje island in the South Sea, South Korea. Strain GYSW-23(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of approximately 2.0-3.0% (w/v) NaCl. Phylogenetic...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.000352
更新日期:2015-09-01 00:00:00
abstract::A collection of eight clinical strains from Belgian hospitals and three clinical strains of the CCUG collection were characterized biochemically as being similar to CDC groups II-h and II-c; the latter differs from group II-h only by positivity for sucrose acidification. These 11 strains were found to cluster accordin...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65158-0
更新日期:2007-11-01 00:00:00
abstract::Among basidiomycetous yeast strains isolated from wilting leaves of various plants in China, two groups of Pseudozyma strains were distinguished from the others by morphological and physiological characterization. Molecular taxonomic analysis based on sequencing of the large subunit (26S) rRNA gene D1/D2 domain and in...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.63827-0
更新日期:2006-01-01 00:00:00
abstract::Five bacterial strains isolated from bats of the family Vespertilionidae were characterized by phenotypic tests and multilocus sequence analysis (MLSA) using the 16S rRNA gene and four housekeeping genes (rpoA, rpoB, infB, recN). Phylogenetic analyses of individual and combined datasets indicated that the five strains...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.062786-0
更新日期:2014-07-01 00:00:00
abstract::Phylogenetic analyses of 16S rRNA gene sequences by distance matrix and parsimony methods indicated that the currently known species of the genus Sphingomonas can be divided into four clusters. Some chemotaxonomic and phenotypic differences were noted among these clusters. Three new genera, Sphingobium, Novosphingobiu...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-51-4-1405
更新日期:2001-07-01 00:00:00
abstract::Phenotypic and phylogenetic studies were performed on six unidentified, Gram-positive, catalase-negative, chain-forming Streptococcus-like organisms recovered from grey seals. Biochemically the six strains were highly related to each other, but they did not appear to correspond to any recognized species of the genus S...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.63082-0
更新日期:2004-09-01 00:00:00
abstract::Strain GI5T was isolated from a surface seawater sample collected from Garorim Bay (West Sea, Republic of Korea). The isolated strain was aerobic, Gram-stain-negative, rod-shaped, motile by means of a polar flagellum, negative for catalase and weakly positive for oxidase. The optimum growth pH, salinity and temperatur...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.002823
更新日期:2018-07-01 00:00:00
abstract::A new, extremely thermophilic bacterium, designated strain MB4T, was isolated from a Chinese hot spring. The new isolate was an obligately anaerobic, rod-shaped, gram-negative, saccharolytic bacterium. Spore formation was not observed. Growth occurred at temperatures between 50 and 80 degrees C, with an optimum of aro...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-51-4-1335
更新日期:2001-07-01 00:00:00
abstract::During a study on the prevalence and diversity of campylobacteria in wild birds faecal samples from the city of Valdivia (southern Chile) 17 Gram-stain-negative, curved-rod-shaped isolates, were initially identified as Campylobacter lari by PCR-RFLP. Further identification by 16S rRNA sequence analysis revealed that t...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001822
更新日期:2017-06-01 00:00:00