Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally-infected does.

Abstract:

BACKGROUND:Brucellosis of goats is caused by Brucella melitensis. It is a re-emerging zoonotic disease in many countries due to transmission from domestic animals and wildlife such as ibex, deer and wild buffaloes. OBJECTIVE:To describe the pathological changes, identification and distribution of B. melitensis in foetuses of experimentally infected does. METHODS:Twelve female goats of approximately 90 days pregnant were divided into 4 groups. Group 1 was exposed intra-conjunctival to 100 µL of sterile PBS while goats of Groups 2, 3 and 4 were similarly exposed to 100 µL of an inoculum containing 109 CFU/mL of live B. melitensis. Goats of these groups were killed at 15, 30 and 60 days post-inoculation, respectively. Foetal fluid and tissues were collected for bacterial identification (using direct bacterial culture, PCR and immuno-peroxidase staining) and histopathological examination. RESULTS:Bilateral intra-conjunctival exposure of pregnant does resulted in in-utero infection of the foetuses. All full-term foetuses of group 4 were either aborted or stillborn, showing petechiations of the skin or absence of hair coat with subcutaneous oedema. The internal organs showed most severe lesions. Immune-peroxidase staining revealed antigen distribution in all organs that became most extensive in group 4. Brucella melitensis was successfully isolated from the stomach content, foetal fluid and various other organs. CONCLUSION:Vertical transmission of caprine brucellosis was evident causing mild to moderate lesions in different organs. The samples of choice for isolation and identification of B. melitensis are stomach content as well as liver and spleen tissue.

journal_name

Vet Q

journal_title

The veterinary quarterly

authors

Mazlan M,Khairani-Bejo S,Hamzah H,Nasruddin NS,Salleh A,Zamri-Saad M

doi

10.1080/01652176.2020.1867328

keywords:

[" Brucella melitensis ","Goat","caprine brucellosis","experimental infection","foetus","pathology","vertical transmission"]

subject

Has Abstract

pub_date

2021-12-01 00:00:00

pages

36-49

issue

1

eissn

0165-2176

issn

1875-5941

journal_volume

41

pub_type

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