Pathogenic potential of Escherichia coli clinical strains from orthopedic implant infections towards human osteoblastic cells.


:Escherichia coli is one of the first causes of Gram-negative orthopedic implant infections (OII), but little is known about the pathogenicity of this species in such infections that are increasing due to the ageing of the population. We report how this pathogen interacts with human osteoblastic MG-63 cells in vitro, by comparing 20 OII E. coli strains to two Staphylococcus aureus and two Pseudomonas aeruginosa strains. LDH release assay revealed that 6/20 (30%) OII E. coli induced MG-63 cell lysis whereas none of the four control strains was cytotoxic after 4 h of coculture. This high cytotoxicity was associated with hemolytic properties and linked to hlyA gene expression. We further showed by gentamicin protection assay and confocal microscopy that the non-cytotoxic E. coli were not able to invade MG-63 cells unlike S. aureus strains (internalization rate <0.01% for the non-cytotoxic E. coli versus 8.88 ± 2.31% and 4.60 ± 0.42% for both S. aureus). The non-cytotoxic E. coli also demonstrated low adherence rates (<7%), the most adherent E. coli eliciting higher IL-6 and TNF-α mRNA expression in the osteoblastic cells. Either highly cytotoxic or slightly invasive OII E. coli do not show the same infection strategies as S. aureus towards osteoblasts.


Pathog Dis


Pathogens and disease


Crémet L,Broquet A,Brulin B,Jacqueline C,Dauvergne S,Brion R,Asehnoune K,Corvec S,Heymann D,Caroff N




Has Abstract


2015-11-01 00:00:00












  • Non-typhoidal Salmonella Typhimurium ST313 isolates that cause bacteremia in humans stimulate less inflammasome activation than ST19 isolates associated with gastroenteritis.

    abstract::Salmonella is an enteric pathogen that causes a range of diseases in humans. Non-typhoidal Salmonella (NTS) serovars such as Salmonella enterica serovar Typhimurium generally cause a self-limiting gastroenteritis whereas typhoidal serovars cause a systemic disease, typhoid fever. However, S. Typhimurium isolates withi...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Carden S,Okoro C,Dougan G,Monack D

    更新日期:2015-06-01 00:00:00

  • Chlamydia trachomatis plasmid-encoded protein pORF5 protects mitochondrial function by inducing mitophagy and increasing HMGB1 expression.

    abstract::Chlamydia trachomatis, an obligate intracellular pathogen, has various effective strategies to regulate host cell death signalling pathways that ensure completion of their growth cycle. Mitochondrial autophagy (mitophagy) is responsible for elimination of dysfunctional and impaired mitochondria, and this process plays...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Lei W,Li Q,Su S,Bu J,Huang Q,Li Z

    更新日期:2017-12-29 00:00:00

  • Leishmania (Viannia) guyanensis in tegumentary leishmaniasis.

    abstract::Leishmania (Viannia) guyanensis is a causal agent of American tegumentary leishmaniasis (ATL). This protozoan has been poorly investigated; however, it can cause different clinical forms of ATL, ranging from a single cutaneous lesion to severe lesions that can lead to destruction of the nasopharyngeal mucosa. L. (V.) ...

    journal_title:Pathogens and disease

    pub_type: 杂志文章,评审


    authors: Borges AF,Gomes RS,Ribeiro-Dias F

    更新日期:2018-06-01 00:00:00

  • Interaction of Cryptosporidium parvum with mouse dendritic cells leads to their activation and parasite transportation to mesenteric lymph nodes.

    abstract::Dendritic cells (DCs) are the antigen-presenting cells capable of activating naïve T cells. Although CD4+ T cells are crucial for Cryptosporidium parvum clearance, little is known about the role of DCs in the immune response to this parasite. In this study, the interaction between mouse DCs and C. parvum was investiga...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Perez-Cordon G,Yang G,Zhou B,Nie W,Li S,Shi L,Tzipori S,Feng H

    更新日期:2014-02-01 00:00:00

  • Simultaneous formulation of influenza vaccine and chitosan nanoparticles within CpG oligodesoxi nucleotides leads to dose-sparing and protects against lethal challenge in the mouse model.

    abstract::Lack of efficient delivery systems for transporting antigenic molecules to the cytosol of antigen-presenting cells presents a major obstacle for antigen uptake by immune cells. To this end, influenza whole inactivated virus vaccines were formulated with chitosan nanoparticles and CpG oligonucleotide as a biodegradable...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Sadati SF,Jamali A,Abdoli A,Abedi-Valugerdi M,Gholami S,Alipour S,Soleymani S,Kheiri MT,Atyabi F

    更新日期:2018-11-01 00:00:00

  • Transcriptional profiling of Vibrio cholerae O1 following exposure to human anti- lipopolysaccharide monoclonal antibodies.

    abstract::Following an episode of cholera, a rapidly dehydrating, watery diarrhea caused by the Gram-negative bacterium, Vibrio cholerae O1, humans mount a robust anti-lipopolysaccharide (LPS) antibody response that is associated with immunity to subsequent re-infection. In neonatal mouse and rabbit models of cholera, passively...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Baranova DE,Willsey GG,Levinson KJ,Smith C,Wade J,Mantis NJ

    更新日期:2020-06-01 00:00:00

  • Role of protein kinase C in cytokine secretion by lung epithelial cells during infection with Paracoccidioides brasiliensis.

    abstract::In this study, we investigated the role of protein kinases C (PKCs) in interleukin (IL)-6 and IL-8 secretion by human lung epithelial A549 cells during infection with the fungal pathogen Paracoccidioides brasiliensis. Rottlerin and the broad spectrum PKC inhibitor Go 6983 reduced cytokine levels in A549 cell-P. brasil...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Alcantara C,Maza PK,Barros BC,Suzuki E

    更新日期:2015-10-01 00:00:00

  • OmpA family proteins and Pmp-like autotransporter: new adhesins of Waddlia chondrophila.

    abstract::Waddlia chondrophila is a obligate intracellular bacterium belonging to the Chlamydiales order, a clade that also includes the well-known classical Chlamydia responsible for a number of severe human and animal diseases. Waddlia is an emerging pathogen associated with adverse pregnancy outcomes in humans and abortion i...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Kebbi-Beghdadi C,Domröse A,Becker E,Cisse OH,Hegemann JH,Greub G

    更新日期:2015-08-01 00:00:00

  • Lung epithelium is the major source of IL-33 and is regulated by IL-33-dependent and IL-33-independent mechanisms in pulmonary cryptococcosis.

    abstract::Inhalation of the fungus Cryptococcus neoformans (C. neoformans) results in pulmonary cryptococcosis associated with IL-33-dependent type 2 immunity. Lung epithelium represents the initial contact site of infection. The role of IL-33 in type 2 immunity has been analyzed, but the source of this cytokine and its effect ...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Heyen L,Müller U,Siegemund S,Schulze B,Protschka M,Alber G,Piehler D

    更新日期:2016-10-01 00:00:00

  • Involvement of toxin-antitoxin modules in Burkholderia cenocepacia biofilm persistence.

    abstract::Biofilms are involved in the recalcitrance of infections due to the presence of persister cells. Although the molecular basis of persistence is still largely unknown, toxin-antitoxin modules (TA) are thought to play a role in this process. In this study, we investigated whether TA modules contribute to persistence tow...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Van Acker H,Sass A,Dhondt I,Nelis HJ,Coenye T

    更新日期:2014-08-01 00:00:00

  • Molecular characterisation of Chlamydia pneumoniae associated to atherosclerosis.

    abstract::Chlamydia pneumoniae is a respiratory pathogen associated with chronic inflammatory diseases such as asthma and atherosclerosis, and its detection in human carotid and coronary atheroma suggests some support for its involvement in atherogenesis. The main objective of our study was to evaluate the association between C...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: El Yazouli L,Criscuolo A,Hejaji H,Bouazza M,Elmdaghri N,Aroussi Alami A,Amraoui A,Dakka N,Radouani F

    更新日期:2017-06-01 00:00:00

  • Recent mouse models and vaccine candidates for preventing chronic/latent tuberculosis infection and its reactivation.

    abstract::Tuberculosis (TB) remains a major challenge in public health worldwide. Until today, the only widely used and approved vaccine is the Mycobacterium bovis bacille Calmette-Guerin (BCG). This vaccine provides a highly variable level of protection against the active, pulmonary form of tuberculosis, and practically none a...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Pedroza-Roldán C,Flores-Valdez MA

    更新日期:2017-08-31 00:00:00

  • In vitro attenuation of a virulent swine isolate of Brachyspira hampsonii.

    abstract::Brachyspira hampsonii causes dysentery-like disease in infected pigs. Serial passage of a virulent swine isolate (P13) one-hundred times in laboratory culture medium was conducted to produce an attenuated strain, and to identify genomic determinants of virulence through comparison of genome sequences of the original a...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Perez JBDS,Fernando C,Nosach RV,Huang Y,Harding JCS,Hill JE

    更新日期:2018-02-01 00:00:00

  • Mce4F Mycobacterium tuberculosis protein peptides can inhibit invasion of human cell lines.

    abstract::This work was aimed at studying the Mycobacterium tuberculosis H37Rv Rv3494c protein, taking into account that it belongs to the mammalian cell entry family (mce) which is thought to have important functions in the disease's pathogenesis. The protein was characterized in silico; its presence on mycobacterial surface w...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Rodríguez DC,Ocampo M,Varela Y,Curtidor H,Patarroyo MA,Patarroyo ME

    更新日期:2015-04-01 00:00:00

  • Enhanced allergic responsiveness after early childhood infection with respiratory viruses: Are long-lived alternatively activated macrophages the missing link?

    abstract::Early childhood infection with respiratory viruses, including human rhinovirus, respiratory syncytial virus (RSV) and influenza, is associated with an increased risk of allergic asthma and severe exacerbation of ongoing disease. Despite the long recognition of this relationship, the mechanism linking viral infection a...

    journal_title:Pathogens and disease

    pub_type: 信件


    authors: Keegan AD,Shirey KA,Bagdure D,Blanco J,Viscardi RM,Vogel SN

    更新日期:2016-07-01 00:00:00

  • Exacerbation of disease by intranasal liquid administration following influenza virus infection in mice.

    abstract::Although numerous studies have clarified the synergistic pathogenesis in mouse models of influenza A virus (IAV)-associated dual infections, fewer studies have investigated the influence of intranasal liquid administration on the disease. This study explored the effects of intranasal PBS administration in mouse models...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Lyu Y,Li P,Yang Z,Zhong N

    更新日期:2020-03-01 00:00:00

  • Simkania negevensis may produce long-lasting infections in human pneumocytes and endometrial cells.

    abstract::Simkania negevensis is a novel Chlamydia-related bacterium and the founding member of the Simkaniaceae family within the Chlamydiales order. Little is known about the biology and pathogenesis of this bacterium. So far, S. negevensis has been considered as an amoebal symbiont, but its natural host remains unknown. More...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Vouga M,Baud D,Greub G

    更新日期:2017-01-01 00:00:00

  • A single-gene approach for the subspecies classification of Mycobacteroides abscessus.

    abstract::The subspecies classification of Mycobacteroides abscessus complex into M. abscessus, M. massiliense and M. bolletii requires the amplification and sequencing of multiple genes. The objective of this study was to evaluate the possibility of subspecies classification using a single PCR target. An in silico study was pe...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Ng HF,Ngeow YF

    更新日期:2020-11-11 00:00:00

  • Cyclic-di-GMP binding induces structural rearrangements in the PlzA and PlzC proteins of the Lyme disease and relapsing fever spirochetes: a possible switch mechanism for c-di-GMP-mediated effector functions.

    abstract::The c-di-GMP network of Borrelia burgdorferi, a causative agent of Lyme disease, consists of Rrp1, a diguanylate cyclase/response regulator; Hpk1, a histidine kinase; PdeA and PdeB, c-di-GMP phosphodiesterases; and PlzA, a PilZ domain c-di-GMP receptor. Borrelia hermsii, a causative agent of tick-borne relapsing fever...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Mallory KL,Miller DP,Oliver LD Jr,Freedman JC,Kostick-Dunn JL,Carlyon JA,Marion JD,Bell JK,Marconi RT

    更新日期:2016-11-01 00:00:00

  • Chronic Pseudomonas aeruginosa biofilm infection impairs murine S100A8/A9 and neutrophil effector cytokines-implications for delayed wound closure?

    abstract::The impact of Pseudomonas aeruginosa biofilm infections in chronic wounds and clinical implication for healing is receiving increased attention. However, the pathophysiology of host/pathogen interplay is not fully understood. By further revealing the mechanisms, necessary new treatment strategies may be identified. Si...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Trøstrup H,Lerche CJ,Christophersen LJ,Thomsen K,Jensen PØ,Hougen HP,Høiby N,Moser C

    更新日期:2017-09-29 00:00:00

  • Peptide matching between Epstein-Barr virus and human proteins.

    abstract::Epstein-Barr virus proteins were examined for amino acid sequence matching to human proteins at the decapeptide level. We report that numerous EBV peptides of different length (from 10- to 13-mer) are present in 28 human proteins. The viral vs. human peptide overlap mainly involves the glycine-rich region allocated in...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Capone G,Calabrò M,Lucchese G,Fasano C,Girardi B,Polimeno L,Kanduc D

    更新日期:2013-12-01 00:00:00

  • Self-reactivity as the necessary cost of maintaining a diverse memory T-cell repertoire.

    abstract::The adaptive immune system is expected to protect the host from infectious agents and malignancies, while avoiding robust activation against self-peptides. However, T cells are notoriously inept at protection whenever the pathogen or tumor is persistent in the body for longer periods of time. While this has been thoug...

    journal_title:Pathogens and disease

    pub_type: 杂志文章,评审


    authors: Singh NJ

    更新日期:2016-10-01 00:00:00

  • Contribution of fibronectin-binding protein to pathogenesis of Streptococcus equi ssp. zooepidemicus.

    abstract::Streptococcus equi ssp. zooepidemicus (S. zooepidemicus) is responsible for a wide variety of infections in many species. Fibronectin-binding protein is a bacterial cell surface protein, which specifically binds fibronectin (FN). Considering the specific role of FN-binding protein in host-pathogen interactions, we inv...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Yi L,Wang Y,Ma Z,Zhang H,Li Y,Zheng JX,Yang YC,Lu CP,Fan HJ

    更新日期:2013-04-01 00:00:00

  • Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood.

    abstract::Staphylococcus epidermidis is frequently associated with the emergence of medical-device-associated bloodstream infections, due to its ability to form biofilms on the surface of vascular catheters. Although these biofilms may be in continuous contact with human blood, how S. epidermidis biofilm cells interact with blo...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: França A,Cerca N

    更新日期:2016-03-01 00:00:00

  • Oral bacteria affect the gut microbiome and intestinal immunity.

    abstract::Recently, it has been suggested that the oral administration of Porphyromonas gingivalis, a keystone pathogen for periodontal disease, induces dysbiosis of the mouse intestinal microbiota and affects intestinal barrier function. Since oral streptococci are the predominant oral bacterial group, we compared the effect o...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Kobayashi R,Ogawa Y,Hashizume-Takizawa T,Kurita-Ochiai T

    更新日期:2020-04-01 00:00:00

  • Hepatic dysfunction induced by intestinal dysbacteriosis mainly manifests as immunologic abnormity in mice.

    abstract::Currently, the potential role of the alterations occurring in the liver immune system and intestinal flora in liver injury remains unknown. Our study aimed to explore the impacts of intestinal microbial barrier damage induced by ceftriaxone on liver immunity. We developed the BALB/c mice model by administering ceftria...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Luo X,Xu B,Xiong T,Su Y,Liu C,Ye Y,Ou M,Zhou L

    更新日期:2020-08-01 00:00:00

  • Cord-forming mycobacteria induce DNA meshwork formation by human peripheral blood mononuclear cells.

    abstract::Mononuclear phagocytes, that is, monocytes and macrophages, are central in the defense against mycobacteria. Mycobacterium abscessus is an opportunistic mycobacterial species able to cause chronic pulmonary infections in patients with cystic fibrosis but also soft tissue infections in immunocompetent individuals. Path...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Jönsson BE,Bylund J,Johansson BR,Telemo E,Wold AE

    更新日期:2013-02-01 00:00:00

  • Saccharomyces boulardii administration can inhibit the formation of gastric lymphoid follicles induced by Helicobacter suis infection.

    abstract::Helicobacter suis has a greater tendency to induce gastric mucosa-associated lymphoid tissue lymphoma compared with other Helicobacter species in humans and animals. Saccharomyces boulardii has been established as an adjunct to H. pylori eradication treatment, but the effect of S. boulardii administration alone on Hel...

    journal_title:Pathogens and disease

    pub_type: 杂志文章


    authors: Yang L,Tian ZB,Yu YN,Zhang CP,Li XY,Mao T,Jing X,Zhao WJ,Ding XL,Yang RM,Zhang SQ

    更新日期:2017-01-01 00:00:00

  • The art of persistence-the secrets to Burkholderia chronic infections.

    abstract::The Gram-negative proteobacteria genus Burkholderia encompasses multiple bacterial species that are pathogenic to humans and other vertebrates. Two pathogenic species of interest within this genus are Burkholderia pseudomallei (Bpm) and the B. cepacia complex (Bcc); the former is the causative agent of melioidosis in ...

    journal_title:Pathogens and disease

    pub_type: 杂志文章,评审


    authors: Lewis ER,Torres AG

    更新日期:2016-08-01 00:00:00

  • Tuberculosis and the art of macrophage manipulation.

    abstract::Macrophages are first-line responders against microbes. The success of Mycobacterium tuberculosis (Mtb) rests upon its ability to convert these antimicrobial cells into a permissive cellular niche. This is a remarkable accomplishment, as the antimicrobial arsenal of macrophages is extensive. Normally bacteria are deli...

    journal_title:Pathogens and disease

    pub_type: 杂志文章,评审


    authors: Upadhyay S,Mittal E,Philips JA

    更新日期:2018-06-01 00:00:00