In vitro orientation of maleSchistosoma mansoni to extracts derived from female schistosomes.

Abstract:

:Chemical orientation of adult maleSchistosoma mansoni was studied during cultivation in vitro. Directional preference was assessed in culture vessels marked with compass coordinates by the application of circular statistics for determining clustering and orientation to a predicted direction. Organic solvent extracts of fresh female schistosomes and supernatant fluids from 72-hr cultures of female parasites were tested for potential chemotactic activity. Analysis showed significant (P < 0.05) directional preference and clustering of male worms towards test compounds at all time periods (24, 48, and 72 hr) with one mixture of female extracts and at one of three time periods with a second female extract. Male worms did not respond to ecdysone, cholesterol, or solvent controls by orienting in predicted direction, although clustering was observed on two of 12 occasions. These results indicate the presence of a chemoattractant compound(s) in female extracts.

journal_name

J Chem Ecol

authors

Childs JE,Shirazian D,Gloer JB,Schiller EL

doi

10.1007/BF01022378

subject

Has Abstract

pub_date

1986-08-01 00:00:00

pages

1729-38

issue

8

eissn

0098-0331

issn

1573-1561

journal_volume

12

pub_type

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