Abstract:
:The aim of this study was to investigate the function of the ING1 gene in lung carcinoma. To detect the inhibitory effect of ING1 in human lung cancer, recombinant ING1b plasmids were transfected into two lung cancer cell lines with different p53 status, A549 with wild-type p53 (wtp53) and SK-MES-1 with mutant p53. Apoptosis, cell cycle, growth rate and the expression of downstream gene p21waf1 were analyzed. In addition, the complex of p33ING1b and p53 was analyzed with coimmunoprecipitation. To detect the gene alteration and the expression of ING1, 70 cases of fresh-frozen lung carcinomas and 217 cases of formalin-fixed, paraffin-embedded specimens were examined for loss of heterozygosity (LOH) and p33ING1b protein expression by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and immunohistochemistry using tissue microarrays, respectively. Overexpression of ING1b inhibited the cell growth of A549 and SK-MES-1, induced cell cycle arrest and apoptosis. p21waf1 was up-regulated and a complex of p33ING1b and wtp53 was found after transfection of ING1b in the wtp53-positive lung cancer cell. High LOH frequency was found in lung carcinomas (55.7%) and p33ING1b expression was lost in 115 of 217 carcinomas (53.0%). Furthermore, there was a highly significant inverse correlation between expression and LOH frequency (P<0.05). ING1 can inhibit the growth of lung cancer cell lines through the induction of cell cycle arrest and apoptosis by forming a complex with wtp53 and up-regulating p21waf1. In human lung cancer, expression of the ING1 gene was reduced or lost and high LOH frequency of ING1 microsatellites was found. The LOH of microsatellites may down-regulate p33ING1b and/or affect its function, thereby, contributing to lung cell carcinogenesis.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Luo ZG,Tang H,Li B,Zhu Z,Ni CR,Zhu MHdoi
10.3892/or.2011.1172subject
Has Abstractpub_date
2011-04-01 00:00:00pages
1073-81issue
4eissn
1021-335Xissn
1791-2431journal_volume
25pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::The long-term effort in investigating chemical methods to eliminate only cancer cells has improved our knowledge and has led to the development of new drugs. The targets for cancer treatment may be large polymeric molecules such as DNA or microtubules as well as regulatory pathways for tumor development and cell survi...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.9.6.1257
更新日期:2002-11-01 00:00:00
abstract::The blood‑brain barrier (BBB) constitutes an efficient organization of tight junctions that limits the delivery of tumor to the brain. The principal tight junction protein in BBB is claudin‑5 (CLDN5), but its mechanism of action remains largely unknown. Long non‑coding RNAs (lncRNAs) are aberrantly expressed in many c...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6208
更新日期:2018-03-01 00:00:00
abstract::Gentian violet (GV) is a cationic triphenylmethane dye, with potent antifungal and antibacterial activity. We recently reported that in vitro GV suppresses the differentiation of bone resorbing osteoclasts while stimulating the differentiation and activity of bone forming osteoblasts. Breast cancer is highly metastati...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4190
更新日期:2015-10-01 00:00:00
abstract::Investigations into the cellular processing of radiolabeled monoclonal antibodies (mAbs) for their further use in radioimmunodiagnosis and cancer therapy are needed in order to understand the fate of internalized and catabolized mAbs. The anti-colorectal cancer mAb, A33, was labelled with 76Br and 125I using the direc...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Rad52 encodes a protein which is required for recombinational repair of double-strand breaks. It is also associated with breast cancer predisposition genes BRCA1 and BRCA2. Mutations in the genes Rad51 or Rad52 result in severe defects in genetic recombination and the repair of double-strand DNA breaks. In order to ex...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::We investigated whether or not the mitochondrial genotypes affect radiation-induced micronucleus (MN) formation. For that purpose, the rho+, KT1 and rho0 human osteosarcoma cell lines were used, which carry the wild-type mitochondrial DNA (mtDNA), the tRNALys mutant mtDNA and no mtDNA, respectively. Despite no signifi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.3.615
更新日期:2001-05-01 00:00:00
abstract::The current study characterized peripheral blood mononuclear cells (PBMC) obtained from leukapheresis products of patients with non-small cell lung cancer (NSCLC) for cytokine release, the ability to incorporate tritiated thymidine following stimulation using PHA as well as the levels of both CD4 and CD8 regulatory T ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000588
更新日期:2009-12-01 00:00:00
abstract::Epigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5008
更新日期:2016-10-01 00:00:00
abstract::Lack of effective therapy is a major problem in the treatment of pancreatic cancer. In the present study, we investigated a natural product, the extract of Pao Pereira (Pao), for its anti-pancreatic cancer effect in vitro and in vivo, either alone or in combination with the first-line chemotherapeutic drug gemcitabine...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2461
更新日期:2013-07-01 00:00:00
abstract::The changes of the microtubular network induced by microtubule destabilizing (Vinblastine, VBL) and stabilizing (Taxol, TAX) agents were studied in NIH/3T3 fibroblastic cells in conventional culture conditions (NIHb) and in a subpopulation (NIHs), obtained after serum deprivation and expressing different morphofunctio...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-12-01 00:00:00
abstract::Glioma is the most common primary brain malignant tumor. Receptor for activated C-kinase 1 (RACK1) is widely expressed in the central nervous system, and regulates multiple cellular processes including cell survival, proliferation, migration and metastasis. However, the role of RACK1 in glioma has never been revealed....
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2723
更新日期:2013-11-01 00:00:00
abstract::The Chinese medicinal herb Sorbaria sorbifolia, native to Changbai Mountain, can induce apoptosis in HepG-2 cells. We studied the mechanism by which 5, 2', 4'-trihydroxy-6,7, 5'-trimethoxyflavone (TTF1) isolated from acetic ether extracts of Sorbaria sorbifolia induces apoptosis in HepG-2 cells. The results showed tha...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1330
更新日期:2011-09-01 00:00:00
abstract::Modulation of the activity of tumor suppressor p53 is a key event in the replication of many viruses. They could manipulate p53 function through modification of phosphorylation for their own purpose. However, there are scarce data on the relationship between high risk human papillomavirus (HPV) E6 protein and p53 phos...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2009-02-01 00:00:00
abstract::MicroRNAs (miRNAs) play crucial roles in the initiation and progression of various cancers, including melanoma. Recently, the genetic variants and deregulation of miR-605 have been reported to participate in carcinogenesis. However, the expression status of the miR-605 in melanoma tissues and its role in melanoma prog...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5740
更新日期:2017-08-01 00:00:00
abstract::SRY-box containing gene 17 (Sox17), a transcription factor, is considered as an antagonist to canonical Wnt/β‑catenin signaling in several types of malignant tumors. As the influence of Sox17 in the pathogenesis of human melanoma is still unknown, the investigation of Sox17 expression in melanoma is warranted and its ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3534
更新日期:2014-12-01 00:00:00
abstract::Miconazole (MIC), an antifungal agent, diplays anti‑tumorigenic activity in various types of human cancers, including bladder cancer, yet its mechanism of antitumor action is not well understood. In the present study, we demonstrated that, in a cell viability assay, MIC had a cytotoxic effect on human T24, J82 and TSG...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5608
更新日期:2017-06-01 00:00:00
abstract::c‑mesenchymal‑epithelial transition (Met) is a transmembrane tyrosine kinase receptor of hepatocyte growth factor (HGF). HGF/Met signaling stimulates numerous pathways, including the Ras/mitogenactivated protein kinase (MAPK), phosphatidylinositol 3‑kinase/protein kinase B and Wnt/β‑catenin pathways, which serve impor...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7799
更新日期:2020-12-01 00:00:00
abstract::Mice are particularly susceptible to carcinogens when exposure starts early in life. We evaluated the expression of stem cell antigen-1 (Sca-1) gene in the lung of variously aged CD-1 mice, either untreated or exposed to environmental cigarette smoke (ECS) and/or to a light source. Sca-1 expression progressively decre...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000458
更新日期:2009-09-01 00:00:00
abstract::Previous studies have shown that S100 calcium-binding protein A8 (S100A8) contributes to the survival and migration of colorectal cancer (CRC) cells. However, whether S100A8 participates in the progression and metastasis of CRC via the regulation of macrophages in the tumor inflammatory microenvironment remains unknow...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4790
更新日期:2016-07-01 00:00:00
abstract::Tetraspanins are transmembrane adaptor proteins involved in the regulation of various fundamental cellular processes. For a number of malignant diseases, the level of expression of members of the tetraspanin family was found to correlate with tumor cell invasiveness, ability to form metastases, and poor clinical outco...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::It has been reported that granulocyte-colony stimulating factor (G-CSF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) can mobilize endothelial progenitor cells (EPCs) in bone marrow cells (BMCs) into peripheral blood (PB) in vivo. Previously, we also reported that macrophage-colony stimulating factor (...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNAs that are involved in tumor initiation and development by suppressing target gene expression. miRNA-154 has been shown to be important in tumorigenesis in many types of cancers. However, its role in osteosarcoma (OS) remains unknown. In the present study, we focused on the r...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4495
更新日期:2016-03-01 00:00:00
abstract::We hypothesized that expression of multidrug resistance-associated protein 2 (MRP2), a major cisplatin transporter, may determine the efficacy of cisplatin as a treatment for patients with hepatocellular carcinoma (HCC). A prospective analysis was conducted of 49 consecutive patients who underwent resection for HCC (1...
journal_title:Oncology reports
pub_type: 临床试验,杂志文章
doi:10.3892/or_00000721
更新日期:2010-04-01 00:00:00
abstract::Combined chemotherapy with 5-fluorouracil and leucovorin (LV) has been widely used for the treatment of patients with colorectal cancer. Given that LV effects are attributable to increased levels of reduced folate in cancer cells, we attempted here to show the in vivo role of folylpolyglutamate synthetase (FPGS), whic...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1206
更新日期:2011-05-01 00:00:00
abstract::Distant metastases represent the major cause of death after curative surgery of colorectal cancer. The aim of this study was to evaluate the role of Smad4 and KRAS genetic alterations in colorectal metastases taking into account both the site (hepatic versus extrahepatic) and the time (synchronous versus metachronous)...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract::High activity 125I brachytherapy has efficacy in selected recurrent malignant brain tumors, but limited efficacy and risk of radiation necrosis have prompted investigation of additional adjunctive therapies. This study aims to assess the toxicity of interstitial conductive hyperthermia used concurrently with 125I brac...
journal_title:Oncology reports
pub_type: 临床试验,杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::We present a case of resected hepatocellular carcinoma (HCC) which invaded the gallbladder with a metastasis to a lymph node. It was extremely difficult to make a differential diagnosis between HCC and gallbladder cancer preoperatively. A 68-year old man was admitted to hospital with complaint of a fever. Ultrasonogra...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.8.2.331
更新日期:2001-03-01 00:00:00
abstract::Colorectal cancer (CRC) is one of the most common cancers worldwide. Long non-coding RNAs (lncRNAs) have been confirmed to play a critical regulatory role in various biological processes including carcinogenesis, which indicates that lncRNAs are valuable biomarkers and therapeutic targets. The novel lncRNA prostate ca...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4364
更新日期:2016-01-01 00:00:00
abstract::The growth of malignant tumors is associated with mechanisms of immune escape and inhibition of apoptosis. Livin is a novel member of the inhibitors of apoptosis (IAP) protein family that inhibits cell apoptosis. Livin is specifically expressed by the majority of tumor cells, but it is not expressed in normal adult ti...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2133
更新日期:2013-02-01 00:00:00
abstract::Recent research indicates that the Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) pathway may play an important role in chronic inflammation which promotes cancer progression, yet the mechanism is not clear. The present study aimed to investigate the role of the JAK/STAT3 pathway in the gr...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3609
更新日期:2015-01-01 00:00:00