Isolation, pure culture and characterization of Serratia symbiotica sp. nov., the R-type of secondary endosymbiont of the black bean aphid Aphis fabae.

Abstract:

:An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic conditions on a rich medium containing 1 % of each of the following substrates: glucose, yeast extract and casein peptone. Pure culture was achieved through the use of solid-phase culture on the same medium and the strain was designated CWBI-2.3(T). 16S rRNA gene sequence analysis revealed that strain CWBI-2.3(T) was a member of the class Gammaproteobacteria, having high sequence similarity (>99 %) with 'Candidatus Serratia symbiotica', the R-type of secondary endosymbiont that is found in several aphid species. As strain CWBI-2.3(T) ( = LMG 25624(T) = DSM 23270(T)) was the first R-type symbiont to be isolated and characterized, it was designated as the type strain of Serratia symbiotica sp. nov.

authors

Sabri A,Leroy P,Haubruge E,Hance T,Frère I,Destain J,Thonart P

doi

10.1099/ijs.0.024133-0

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

2081-2088

issue

Pt 9

eissn

1466-5026

issn

1466-5034

journal_volume

61

pub_type

杂志文章
  • The status of the species Enterobacter siamensisKhunthongpan et al. 2014. Request for an Opinion.

    abstract::In the course of a taxonomic study describing novel species of the genus Enterobacter it was found that the 16S rRNA gene sequence of the type strain of Enterobacter siamensis, obtained both directly from the authors of the publication on Enterobacter siamensis and from the Korean Collection for Type Cultures (C2361T ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000777

    authors: Kämpfer P,Doijad S,Chakraborty T,Glaeser SP

    更新日期:2016-01-01 00:00:00

  • Cryptococcus nemorosus sp. nov. and Cryptococcus perniciosus sp. nov., related to Papiliotrema Sampaio et al. (Tremellales).

    abstract::Three mycocinogenic strains representing the genus Cryptococcus were isolated on glucuronate agar from plants and turf collected in the Prioksko-terrasny biosphere reserve (Russia). These isolates fit the standard description of Cryptococcus laurentii, but differ from its type strain in both their mycocin-sensitivity ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02374-0

    authors: Golubev WI,Gadanho M,Sampaio JP,Golubev NW

    更新日期:2003-05-01 00:00:00

  • Mobilicoccus caccae sp. nov., isolated from the faeces of the primate Rhinopithecus roxellanae.

    abstract::A novel actinobacterium, designated YIM 101593T, was isolated from the faeces of a primate (Rhinopithecus roxellanae) living in Yunnan Wild Animal Park in Yunnan province, south-west China. The isolate was Gram-stain-positive, facultatively anaerobic, coccus-shaped, oxidase-negative and motile. The cell wall contained...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001939

    authors: Chen X,Li QY,Li GD,Xu FJ,Jiang CL,Han L,Huang XS,Jiang Y

    更新日期:2017-07-01 00:00:00

  • Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand.

    abstract::Two strains (S-34T and S-35) of a novel ascomycetous yeast species belonging to the genus Kazachstania were isolated from soil from a mixed deciduous forest in Amphoe Wang Nam Khiao, Nakhon Ratchasima Province, Thailand. The D1/D2 domains of the large-subunit rDNA sequences of the two strains were identical and also i...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64539-0

    authors: Limtong S,Yongmanitchai W,Tun MM,Kawasaki H,Seki T

    更新日期:2007-02-01 00:00:00

  • Paracoccus caeni sp. nov., isolated from sludge.

    abstract::A Gram-negative, non-motile bacterium, designated MJ17(T), was isolated from sludge at the Daejeon sewage disposal plant in South Korea. Comparative 16S rRNA gene sequence analysis showed that strain MJ17(T) belonged to the genus Paracoccus in the family Rhodobacteraceae of the class Alphaproteobacteria. 16S rRNA gene...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.017897-0

    authors: Lee M,Woo SG,Park G,Kim MK

    更新日期:2011-08-01 00:00:00

  • Methanocalculus pumilus sp. nov., a heavy-metal-tolerant methanogen isolated from a waste-disposal site.

    abstract::A mesophilic hydrogenotrophic methanogen, strain MHT-1T, was isolated from the leachate of a sea-based site for solid waste disposal (the port of Osaka, Japan). Strain MHT-1T was found to be an irregular coccus and was able to use H2/CO2 and formate as energy sources. Acetate was required for growth. The optimum tempe...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-50-5-1723

    authors: Mori K,Yamamoto H,Kamagata Y,Hatsu M,Takamizawa K

    更新日期:2000-09-01 00:00:00

  • Noviherbaspirillum agri sp. nov., isolated from reclaimed grassland soil, and reclassification of Herbaspirillum massiliense (Lagier et al., 2014) as Noviherbaspirillum massiliense comb. nov.

    abstract::A straw-coloured, Gram-staining-negative, aerobic, motile and rod-shaped bacterium, designated strain K-1-15T, was isolated from reclaimed grassland soil from Biratnagar, Morang, Nepal. This strain was non-spore-forming, catalase-negative and oxidase-positive. It was able to grow at 10-45 °C, pH 6.5-9.5 and 0-1.5 % (w...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001747

    authors: Chaudhary DK,Kim J

    更新日期:2017-05-01 00:00:00

  • Streptomyces xinghaiensis sp. nov., isolated from marine sediment.

    abstract::A novel actinomycete, strain S187(T), was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0-9 % NaCl and at pH 6.0-9.0 and 10-45 degrees C. The cell wall of strain S187(T) contained the isomer ll-diaminopimelic acid as the diagnostic diamino ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.009878-0

    authors: Zhao XQ,Li WJ,Jiao WC,Li Y,Yuan WJ,Zhang YQ,Klenk HP,Suh JW,Bai FW

    更新日期:2009-11-01 00:00:00

  • Rhodococcus pedocola sp. nov. and Rhodococcus humicola sp. nov., two antibiotic-producing actinomycetes isolated from soil.

    abstract::Two novel actinobacterial strains, UC12T and UC33T, were isolated from forest topsoil in Suwon, Gyeonggi-Do, South Korea. Comparative analysis of nearly full-length 16S rRNA gene sequences of UC12T and UC33T revealed close pairwise similarity with species of the genus Rhodococcus, and the UC12T and UC33T sequences wer...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001039

    authors: Nguyen TM,Kim J

    更新日期:2016-06-01 00:00:00

  • Kuraishia mediterranea sp. nov., a methanol-assimilating yeast species from olive oil and its sediment.

    abstract::Six yeast strains isolated from olive oil sediments and spoiled olive oils originating from Slovenia and Portugal, respectively, proved to represent an undescribed yeast species based on DNA sequence comparisons. The analysis of gene sequences for internal transcribed spacer regions and the large subunit rRNA gene D1/...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002392

    authors: Čadež N,Dlauchy D,Tóbiás A,Péter G

    更新日期:2017-11-01 00:00:00

  • Bacteroides intestinalis sp. nov., isolated from human faeces.

    abstract::During studies of the microbiota of human faeces, five strains of Gram-negative anaerobic rods were isolated following growth in a polyamine-deficient medium. These strains belonged to the genus Bacteroides on the basis of 16S rRNA gene sequence data. 16S rRNA gene sequence similarity between one of the strains, 341T,...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63914-0

    authors: Bakir MA,Kitahara M,Sakamoto M,Matsumoto M,Benno Y

    更新日期:2006-01-01 00:00:00

  • Flavobacterium alkalisoli sp. nov., isolated from rhizosphere soil of Suaeda salsa.

    abstract::A Gram-negative, strictly aerobic, gliding motility, none-spore forming, yellow, rods bacterial strain, designated XS-5T, was isolated from rhizosphere soil of Suaeda salsa, in Tumd Right Banner, Inner Mongolia, PR China. A phylogenetic tree based on the 16S rRNA gene sequences and the phylogenomic tree both showed th...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004255

    authors: Xu L,Wang HT,Zhang JX,Zhang H,Wang S,Sun JQ

    更新日期:2020-06-01 00:00:00

  • Cohnella xylanilytica sp. nov. and Cohnella terrae sp. nov., xylanolytic bacteria from soil.

    abstract::Two xylan-degrading bacteria, strains MX15-2(T) and MX21-2(T), were isolated from soils collected in Nan province, Thailand. Cells were Gram-reaction-positive, facultatively anaerobic, spore-forming and rod-shaped. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-7. ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.017855-0

    authors: Khianngam S,Tanasupawat S,Akaracharanya A,Kim KK,Lee KC,Lee JS

    更新日期:2010-12-01 00:00:00

  • Jiulongibacter sediminis gen. nov., sp. nov., a new member of the family Cytophagaceae, isolated from the surface sediment of the Jiulong River in China.

    abstract::A bacterial strain, designated JN14-9T, was isolated from surface sediment of the Jiulong River and characterized in a taxonomic study using a polyphasic approach. Strain JN14-9T was Gram-stain-negative, non-motile, rod-shaped and orange-pigmented. It can grow at 15-37 °C (optimum 25-30 °C), at pH 7-8 (optimum pH 7) a...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001038

    authors: Liu Y,Du J,Lai Q,Dong C,Xie Y,Shao Z

    更新日期:2016-06-01 00:00:00

  • Novosphingobium taihuense sp. nov., a novel aromatic-compound-degrading bacterium isolated from Taihu Lake, China.

    abstract::A novel aromatic-compound-degrading bacterium, strain T3-B9(T), was isolated from sediment of Taihu Lake, Jiangsu Province, south-east China. This bacterial isolate assimilated several aromatic compounds such as phenol, aniline, nitrobenzene, 4-chloronitrobenzene and phenanthrene. The taxonomy of strain T3-B9(T) was s...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63468-0

    authors: Liu ZP,Wang BJ,Liu YH,Liu SJ

    更新日期:2005-05-01 00:00:00

  • Anaerobranca zavarzinii sp. nov., an anaerobic, alkalithermophilic bacterium isolated from Kamchatka thermal fields.

    abstract::A novel obligately anaerobic, alkalithermophilic, chemo-organotrophic bacterium was isolated from a small and very shallow geothermally heated pool at Pushino (Kamchatka, Far East Russia). The bacterium, designated strain JW/VK-KS5Y(T), was a Gram staining negative, Gram type positive rod. The cells were sometimes bra...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65364-0

    authors: Kevbrin V,Boltyanskaya Y,Garnova E,Wiegel J

    更新日期:2008-06-01 00:00:00

  • Dissection of the genus Methylibium: reclassification of Methylibium fulvum as Rhizobacter fulvus comb. nov., Methylibium aquaticum as Piscinibacter aquaticus gen. nov., comb. nov. and Methylibium subsaxonicum as Rivibacter subsaxonicus gen. nov., comb. n

    abstract::16S rRNA gene sequenced-based phylogeny indicates that Rhizobacter dauci ATCC 43778(T) branches within the radiation of Methylibium type strains. A comparative chemotaxonomic study including fatty acid methyl esters, polar lipids and polyamines reveals significant differences that, in combination with the topology of ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.008383-0

    authors: Stackebrandt E,Verbarg S,Frühling A,Busse HJ,Tindall BJ

    更新日期:2009-10-01 00:00:00

  • Kitasatospora sampliensis sp. nov., a novel actinobacterium isolated from soil of a sugar-cane field in India.

    abstract::Polyphasic characterization of an actinomycete strain VT-36(T) isolated from a sugar-cane field soil sample collected in Punjab State, India, revealed that the strain belongs to the genus Kitasatospora. The strain's chemotaxonomic characters and G+C content of DNA (76.5 mol%) were typical of members of the genus. Anal...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63836-0

    authors: Mayilraj S,Krishnamurthi S,Saha P,Saini HS

    更新日期:2006-03-01 00:00:00

  • Psychrobacter pygoscelis sp. nov. isolated from the penguin Pygoscelis papua.

    abstract::One slightly beige-white pigmented, Gram-stain-negative, rod-shaped bacterium, strain I-STPP5bT, was isolated from the trachea of a Gentoo penguin chick individual (Pygoscelin papua) investigated in Fildes Bay, Chilean Antarctic (62° 12' S, 58° 57' W). I-STPP5bT consists of a 3.4 Mb chromosome with a DNA G+C content o...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003739

    authors: Kämpfer P,Glaeser SP,Irgang R,Fernández-Negrete G,Poblete-Morales M,Fuentes-Messina D,Cortez-San Martín M,Avendaño-Herrera R

    更新日期:2020-01-01 00:00:00

  • Photobacterium histaminum Okuzumi et al. 1994 is a later subjective synonym of Photobacterium damselae subsp. damselae (Love et al. 1981) Smith et al. 1991.

    abstract::The type strain of Photobacterium histaminum, JCM 8968T (= ATCC 51805T), and that of Photobacterium damselae subsp. damselae, ATCC 33539T, exhibit 100% identity in their 16S rRNA sequence, more than 80% DNA-DNA homology and only one phenotypic difference. Also, like P. histaminum, P. damselae subsp. damselae was shown...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-50-3-1339

    authors: Kimura B,Hokimoto S,Takahashi H,Fujii T

    更新日期:2000-05-01 00:00:00

  • Corallincola holothuriorum sp. nov., a facultative anaerobe isolated from sea cucumber intestine.

    abstract::Strain C4T, isolated from sea cucumber intestine in Weihai, Shandong, PR China, is a novel Gram-stain-negative, facultatively anaerobic, amphitrichously flagellated, short rod that grows as creamy white bacterial colonies on plates. Optimal growth of the strain was observed at 28-30 °C, pH 6.5-7.0 and at a concentrati...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003456

    authors: Xia HF,Chen GJ,Du ZJ

    更新日期:2019-08-01 00:00:00

  • Pedobacter humicola sp. nov., a member of the genus Pedobacter isolated from soil.

    abstract::An aerobic, Gram-stain-negative, oxidase-negative, catalase-positive, non-motile, non-spore-forming, rod-shaped, light pink-pigmented bacterium, designated strain R135T, was isolated from soil in Hwaseong, South Korea. Flexirubin-type pigments were absent. Phylogenetic analysis based on the 16S rRNA gene sequence reve...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001010

    authors: Dahal RH,Kim J

    更新日期:2016-06-01 00:00:00

  • Sulfurivermis fontis gen. nov., sp. nov., a sulfur-oxidizing autotroph, and proposal of Thioprofundaceae fam. nov.

    abstract::A novel Gram-stain-negative, chemolithoautotrophic sulfur oxidizer, strain JG42T, was isolated from a hot spring microbial mat. As an electron donor for autotrophic growth, strain JG42T utilized sulfide, thiosulfate, tetrathionate and elemental sulfur. Cells of strain JG42T were oxidase-positive and catalase-negative....

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002137

    authors: Kojima H,Watanabe M,Fukui M

    更新日期:2017-09-01 00:00:00

  • Polynucleobacter hirudinilacicola sp. nov. and Polynucleobacter campilacus sp. nov., both isolated from freshwater systems.

    abstract::Strains MWH-EgelM1-30-B4T and MWH-Feld-100T were isolated from the water columns of two freshwater systems. Both strains represent delicate bacteria not easy to work with in laboratory experiments. Phylogenetic analyses of the 16S rRNA genes suggested that both strains were affiliated with the genus Polynucleobacter. ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002880

    authors: Hahn MW,Koll U,Schmidt J,Huymann LR,Karbon G,Lang E

    更新日期:2018-08-01 00:00:00

  • Herbidospora sakaeratensis sp. nov., isolated from soil, and reclassification of Streptosporangium claviforme as a later synonym of Herbidospora cretacea.

    abstract::An actinomycete strain, DMKUA 205(T), was isolated from a soil sample collected from the Sakaerat Biosphere Reserve in Nakhonratchasima Province, Thailand. The novel strain produced short chains of non-motile spores on the tips of long sporophores branching from the vegetative hyphae. The morphological and chemotaxono...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.024315-0

    authors: Boondaeng A,Suriyachadkun C,Ishida Y,Tamura T,Tokuyama S,Kitpreechavanich V

    更新日期:2011-04-01 00:00:00

  • Acetobacter ghanensis sp. nov., a novel acetic acid bacterium isolated from traditional heap fermentations of Ghanaian cocoa beans.

    abstract::Twenty-three acetic acid bacteria, isolated from traditional heap fermentations of Ghanaian cocoa beans, were subjected to a polyphasic taxonomic study. The isolates were catalase-positive, oxidase-negative, Gram-negative rods. They oxidized ethanol to acetic acid and were unable to produce 2-ketogluconic acid, 5-keto...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64840-0

    authors: Cleenwerck I,Camu N,Engelbeen K,De Winter T,Vandemeulebroecke K,De Vos P,De Vuyst L

    更新日期:2007-07-01 00:00:00

  • Rhizobium alamii sp. nov., an exopolysaccharide-producing species isolated from legume and non-legume rhizospheres.

    abstract::A group of exopolysaccharide-producing bacteria was isolated from the root environment of Arabidopsis thaliana. The genetic diversity revealed by REP-PCR fingerprinting indicated that the isolates correspond to different strains. 16S rRNA gene sequence analysis showed that the isolates are closely related to the strai...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000521-0

    authors: Berge O,Lodhi A,Brandelet G,Santaella C,Roncato MA,Christen R,Heulin T,Achouak W

    更新日期:2009-02-01 00:00:00

  • Winogradskyella rapida sp. nov., isolated from protein-enriched seawater.

    abstract::Flavobacteria are emerging as an important group of organisms associated with the degradation of complex organic matter in aquatic environments. A novel Gram-reaction-negative, heterotrophic, rod-shaped, aerobic, yellow-pigmented and gliding bacterium, strain SCB36T, was isolated from a protein-enriched seawater sampl...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.008334-0

    authors: Pinhassi J,Nedashkovskaya OI,Hagström A,Vancanneyt M

    更新日期:2009-09-01 00:00:00

  • Skermanella aerolata sp. nov., isolated from air, and emended description of the genus Skermanella.

    abstract::A light-pink-coloured bacterium, designated strain 5416T-32(T), was isolated from an air sample in Korea. Cells of the strain were strictly aerobic, Gram-negative, motile (single polar or subpolar flagellum) and rod-shaped. Optimal growth occurred at 25-30 degrees C and at pH 6.0-7.0. The major quinones were Q-10 and ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64676-0

    authors: Weon HY,Kim BY,Hong SB,Joa JH,Nam SS,Lee KH,Kwon SW

    更新日期:2007-07-01 00:00:00

  • Larkinella ripae sp. nov., isolated from seashore soil.

    abstract::Strain 15J11-11T was isolated from soil collected at the seashore and was Gram-staining-negative, short-rod-shaped, gliding and pale-pink pigmented. Flexirubin-type pigments were absent. The isolate grew at a temperature range of 15 to 30 °C and a pH range of 7 to 8. Comparative 16S rRNA gene sequence studies showed t...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002188

    authors: Lee YH,Lee JJ,Lee SY,Lee DS,Kim MK,Ten LN,Jung HY

    更新日期:2017-10-01 00:00:00